Whole Cells Imaged by Hard X-ray Transmission Microscopy

Liang, Zhiting; Guan, Yong; Chen, Shan; Tian, Yangchao

doi:10.1007/978-3-319-22437-4_5

Cited by 1 publication

(1 citation statement)

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“…For imaging of cellular morphology, hard X-ray microscopy thus relies on staining or phase contrast methods. As with electron microscopy, chemical staining for X-ray microscopy could be used to visualize the structure of cells [63]. But for imaging of morphological changes in cell-cell or cell-environment studies, chemical staining is a potential source of artifacts, making interpretation of the data unreliable.…”

Section: External Morphologymentioning

confidence: 99%

Imaging cell morphology and physiology using X-rays

Weinhardt

Chen

Ekman

et al. 2019

Biochemical Society Transactions

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Morphometric measurements, such as quantifying cell shape, characterizing sub-cellular organization, and probing cell-cell interactions, are fundamental in cell biology and clinical medicine. Until quite recently, the main source of morphometric data on cells has been light- and electron-based microscope images. However, a number of technological advances have propelled X-ray microscopy into becoming another source of high-quality morphometric information. Here we review the status of X-ray microscopy as a quantitative biological imaging modality. We also describe the combination of X-ray microscopy data with information from other modalities to generate polychromatic views of biological systems. For example, the amalgamation of molecular localization data, from fluorescence microscopy or spectromicroscopy, with structural information from X-ray tomography. This combination of data from the same specimen generates a more complete picture of the system than can be obtained by a single microscopy method. Such multimodal combinations greatly enhance our understanding of biology by combining physiological and morphological data to create models that more accurately reflect the complexities of life.

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Section: External Morphologymentioning

confidence: 99%