Whole-cell-reporter-gene-based biosensing systems on a compact disk microfluidics platform

Rothert, Anna; Deo, Sapna K.; Millner, Lori M.; Puckett, Libby G.; Madou, Marc; Daunert, Sylvia

doi:10.1016/j.ab.2004.10.048

Cited by 63 publications

(32 citation statements)

References 21 publications

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“…Several examples of detection of different classes of analytes, ranging from heavy metals to endocrine disruptors have been reported. 5,6 Nonetheless, the majority of previously reported devices lack adequate analytical performance for real-life applications, and have thus failed to reach the market. 7,8 To increase the robustness of such devices, several cell immobilization strategies have been investigated, aiming to keep the cells alive and responsive to the target analyte.…”

Section: Introductionmentioning

confidence: 99%

Bioengineered bioluminescent magnetotactic bacteria as a powerful tool for chip-based whole-cell biosensors

et al. 2013

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This paper describes the generation of genetically engineered bioluminescent magnetotactic bacteria (BL-MTB) and their integration into a microfluidic analytical device to create a portable toxicity detection system. Magnetospirillum gryphiswaldense strain MSR-1 was bioengineered to constitutively express a red-emitting click beetle luciferase whose bioluminescent signal is directly proportional to bacterial viability. The magnetic properties of these bacteria have been exploited as "natural actuators" to transfer the cells in the chip from the reaction to the detection area, optimizing the chip's analytical performance. A robust and cost-effective biosensor for the evaluation of sample toxicity, named MAGNETOX, based on lens-free contact imaging detection, has been developed. A microfluidic chip has been fabricated using multilayered black and transparent polydimethyl siloxane (PDMS) in which BL-MTB are incubated for 30 min with the sample, then moved by microfluidics, trapped, and concentrated in detection chambers by an array of neodymium-iron-boron magnets. The chip is placed in contact with a cooled CCD via a fiber optic taper to perform quantitative bioluminescence imaging after addition of luciferin substrate. A model toxic compound (dimethyl sulfoxide, DMSO) and a bile acid (taurochenodeoxycholic acid, TCDCA) were used to investigate the analytical performance of the MAGNETOX. Incubation with DMSO and TCDCA drastically reduces the bioluminescent signal in a dose-related manner. The generation of bacteria that are both magnetic and bioluminescent combines the advantages of easy 2D cell handling with ultra sensitive detection, offering undoubted potential to develop cell-based biosensors integrated into microfluidic chips.

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Section: Introductionmentioning

confidence: 99%

Bioengineered bioluminescent magnetotactic bacteria as a powerful tool for chip-based whole-cell biosensors

et al. 2013

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“…One other kit using the inducible bioluminescent strains for arsenic or mercury (Biologically Heavy metal Assay kit) is distributed by Aboatox company following the works of Tauriainen et al (1997Tauriainen et al ( , 1998. The team of S.Daunert (Lexinton, Irvine, the USA) developed an original microfluidic and analysis system integrated in a compact disc allowing arsenic detection with the arsenicsensitive E.coli pSD10 (parsR:: arsR:: gfpuv) (Rothert et al, 2005 (Riether, Dollard et al, 2001) DL E.coli pJAMA-arsR (Stocker et al, 2003) Cd …”

Section: 3mentioning

confidence: 99%

Bacterial Bioluminescent Biosensor Characterisation for On-line Monitoring of Heavy Metals Pollutions in Waste Water Treatment Plant Effluents

Charrier¹,

Durand²,

Affi³

et al. 2010

Biosensors

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“…The broad generality of the centrifugal disks makes them applicable to many conventional detection modes, including matrixassisted laser desorption/ionization, 60,61 fluorescent assays using green fluorescent protein, 62 enzyme-linked immunosorbent assays ͑ELISA͒, 63 whole-cell reporter gene assays, 64 fluorescent antibody assays, 65 fluorescent detection of beads and quantum dots, 66 and blood absorbance measurements using in-disk optical paths. 67 The biodisks can be used as ionic biosensors.…”

Section: Detection Modes and Applications Of Biodisksmentioning

confidence: 99%

Invited Review Article: Review of centrifugal microfluidic and bio-optical disks

Nolte

2009

Review of Scientific Instruments

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Spinning biodisks have advantages that make them attractive for specialized biochip applications. The two main classes of spinning biodisks are microfluidic disks and bio-optical compact disks ͑BioCD͒. Microfluidic biodisks take advantage of noninertial pumping for lab-on-a-chip devices using noninertial valves and switches under centrifugal and Coriolis forces to distribute fluids about the disks. BioCDs use spinning-disk interferometry, under the condition of common-path phase quadrature, to perform interferometric label-free detection of molecular recognition and binding. The optical detection of bound molecules on a disk is facilitated by rapid spinning that enables high-speed repetitive sampling to eliminate 1 / f noise through common-mode rejection of intensity fluctuations and extensive signal averaging. Multiple quadrature classes have been developed, such as microdiffraction, in-line, phase contrast, and holographic adaptive optics. Thin molecular films are detected through the surface dipole density with a surface height sensitivity for the detection of protein spots that is approximately 1 pm. This sensitivity easily resolves a submonolayer of solid-support immobilized antibodies and their antigen targets. Fluorescence and light scattering provide additional optical detection techniques on spinning disks. Immunoassays have been applied to haptoglobin using protein A/G immobilization of antibodies and to prostate specific antigen. Small protein spots enable scalability to many spots per disk for high-throughput and highly multiplexed immonoassays.

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Whole-cell-reporter-gene-based biosensing systems on a compact disk microfluidics platform

Cited by 63 publications

References 21 publications

Bioengineered bioluminescent magnetotactic bacteria as a powerful tool for chip-based whole-cell biosensors

Bioengineered bioluminescent magnetotactic bacteria as a powerful tool for chip-based whole-cell biosensors

Bacterial Bioluminescent Biosensor Characterisation for On-line Monitoring of Heavy Metals Pollutions in Waste Water Treatment Plant Effluents

Invited Review Article: Review of centrifugal microfluidic and bio-optical disks

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