Abstract:By exploiting versatile P450 enzymes, whole-cell biocatalysis can be performed to synthesize valuable compounds in Escherichia coli. However, the insufficient supply of heme limits the whole-cell P450 biocatalytic activity. Here we report a strategy of improving intracellular heme supply for enhancing the catalytic efficiencies of P450s. After comparing the effects of enhancing heme transport and biosynthesis on P450 activities, intracellular heme supply was optimized through the integrated expression of neces… Show more
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