Whole-cell evaluation of urease activity of Pararhodobacter sp. isolated from peripheral beachrock

Fujita, Masahiro; Nakashima, Kazunori; Achal, Varenyam; Kawasaki, Satoru

doi:10.1016/j.bej.2017.04.004

Cited by 44 publications

(52 citation statements)

References 30 publications

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“…The similar tendency is reported for Pararhodobacter sp. [27], Deleya venusta [42] and Strep-tococcus salivarius [43]. It is worth to note that the Lysinibacillus xylanilyticus reported herein exhibited a distinct behavior regarding urease activity, in which the urease enzyme is highly heat inactivated; urease is not stable even at 30 °C.…”

Section: Discussionmentioning

confidence: 79%

“…Numerous investigators have studied the effect of temperature on the rate of urea hydrolysis of many soil bacteria [23,27,30,40]. In general, their results have indicated that the rate of urea hydrolysis increases with increasing temperature, suggesting that the urease of most of the soil bacteria is not heat inactivated (up to the temperature ranging 45-60 °C).…”

Section: Discussionmentioning

confidence: 99%

“…Similar phenomena could be seen on certain microbial urease like Sporosarcina pasteurii [23,40], Pararhodobacter sp. [27] and Klebsiella aerogenes [44], those have the optimum activity under weak alkaline conditions.…”

Section: Discussionmentioning

confidence: 99%

“…This is similar to that reported to most of the urease of the species including Sporosarcina and Pararhodobacter sp. [27,41].…”

Section: Enzyme Locationmentioning

confidence: 99%

“…Only a few MICP studies focused on introducing some other ureolytic strains including Bacillus sphaercus (MCP-11) [26], Pararhodobactor sp. [9,27], Bacillus sp. VS1 [28,29] and B. megaterium (ATCC 14581) [14].…”

Section: Introductionmentioning

confidence: 99%

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Feasibility study for slope soil stabilization by microbial induced carbonate precipitation (MICP) using indigenous bacteria isolated from cold subarctic region

et al. 2019

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Microbial induced carbonate precipitation (MICP) is relatively an innovative soil improvement technique, learnt from the bio-mediated geochemical reactions that naturally occur in the earth surface. During the MICP, CaCO 3 is metabolically precipitated in soil pores, cement the particle contacts and improves the strength and stiffness of soil. Environment temperature is one of the most key factors that determines the efficiency MICP. The purpose of this study is to investigate the feasibility of stabilizing the slope soil of cold subarctic region (Hokkaido, Japan). The implication of MICP in cold subarctic zones remains as a major challenge, as the enzymatic performance of the bacteria typically declines during lower temperatures hence insufficient formation of CaCO 3 in soil matrix. Therefore, as a potential approach, this study attempted to investigate the feasibility of using the bacteria which have been adapted to native cold climatic conditions. The objectives of this paper are evaluating (1) the effect of temperature in bacterial response, and (2) the effect of grain size distribution in cementation mechanism. The observations suggest that the enzyme activity of the bacteria is negligible at and above 30 °C, whereas it is significant at relatively lower temperatures. The comparison of treated soils suggests that the fine content in slope soil increased number of particle contacts, facilitated effective packing, and promoted the effectiveness of MICP compared to that of uniformly graded sands. Finally, the technical feasibility in slope soil stabilization was well demonstrated using model solidification test. The limitations in stabilizing the slope are also discussed in detail.

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Section: Discussionmentioning

confidence: 79%