Whole cell biotransformation for reductive amination reactions

Klatte, Stephanie; Lorenz, Elisabeth; Wendisch, Volker F.

doi:10.4161/bioe.27151

Cited by 15 publications

(7 citation statements)

References 31 publications

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“…An ideal whole-cell biocatalyst should be genetically amenable, grow fast in simple media, produce large amounts of the enzyme, and be compatible with subsequent downstream processing regimens (17). Thus, optimization of biochemical properties of the whole-cell biocatalyst for superior production is vital in whole-cell biotransformation processes.…”

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confidence: 99%

Metabolic Engineering of Raoultella ornithinolytica BF60 for Production of 2,5-Furandicarboxylic Acid from 5-Hydroxymethylfurfural

Hossain

Yuan

et al. 2017

Appl Environ Microbiol

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2,5-Furandicarboxylic acid (FDCA) is an important renewable biotechnological building block because it serves as an environmentally friendly substitute for terephthalic acid in the production of polyesters. Currently, FDCA is produced mainly via chemical oxidation, which can cause severe environmental pollution. In this study, we developed an environmentally friendly process for the production of FDCA from 5-hydroxymethyl furfural (5-HMF) using a newly isolated strain, Raoultella ornithinolytica BF60. First, R. ornithinolytica BF60 was identified by screening and was isolated. Its maximal FDCA titer was 7.9 g/liter, and the maximal molar conversion ratio of 5-HMF to FDCA was 51.0% (mol/mol) under optimal conditions (100 mM 5-HMF, 45 g/liter whole-cell biocatalyst, 30°C, and 50 mM phosphate buffer [pH 8.0]). Next, dcaD, encoding dicarboxylic acid decarboxylase, was mutated to block FDCA degradation to furoic acid, thus increasing FDCA production to 9.2 g/liter. Subsequently, aldR, encoding aldehyde reductase, was mutated to prevent the catabolism of 5-HMF to HMF alcohol, further increasing the FDCA titer, to 11.3 g/liter. Finally, the gene encoding aldehyde dehydrogenase 1 was overexpressed. The FDCA titer increased to 13.9 g/liter, 1.7 times that of the wild-type strain, and the molar conversion ratio increased to 89.0%.IMPORTANCE In this work, we developed an ecofriendly bioprocess for green production of FDCA in engineered R. ornithinolytica. This report provides a starting point for further metabolic engineering aimed at a process for industrial production of FDCA using R. ornithinolytica.

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confidence: 99%

Metabolic Engineering of Raoultella ornithinolytica BF60 for Production of 2,5-Furandicarboxylic Acid from 5-Hydroxymethylfurfural

Hossain

Yuan

et al. 2017

Appl Environ Microbiol

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“…Mucus is relatively rich in N, P, and K. Mucus N is mainly derived from substances such as NH 4 + , urea, small molecule metabolites, amino acids, and proteins 26,28 . In contrast, mucus P is derived from bacterial microorganisms and organophosphorus compounds 7 , while mucus K is mainly in cytoplasm and electrolytes 29,30 . Under 5 V voltage stimulation, the earthworm body surface normally released mucus, which contains various nutrients required by plants.…”

Section: Resultsmentioning

confidence: 99%

Compositional changes and ecological characteristics of earthworm mucus under different electrical stimuli

Huan

Wang

Chu

et al. 2023

Sci Rep

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Earthworm mucus is rich in nutrients that can initiate the mineralization and humification of organic matter and is of great importance for contaminated soil remediation and sludge reutilization. In this study, six voltage and current combinations were utilized to promote earthworm mucus production (5 V and 6 V at 10, 20 and 30 mA, respectively), to explore the compositional changes of the mucus produced under different electrical stimuli, and to propose the best electrical stimulation group and mucus fraction applicable to soil heavy metal pollution remediation and sludge reutilization. The results showed that the mucus produced by the six electrical stimuli was mainly composed of proteins, amino acids, carbohydrates, fatty acids, and polysaccharides, with small amounts of alcohol, phenol, and ester organic substances. Under different electrical stimuli, each component changed significantly (P < 0.05). pH and conductivity were higher at 6 V 20 mA, total nitrogen and phosphorus contents reached their maximum at 5 V 30 mA, and total potassium at 6 V 10 mA. Protein, amino acids, and carbohydrates were most abundant in the mucus produced at 5 V 10 mA, while trace metal elements reached their lowest values at 5 V 10 mA. Finally, based on principal component analysis and combined with previous studies, it was concluded that the mucus produced at 5 V 10 mA was weakly alkaline, high in amino acids and nutrients and low in trace metal elements, and most suitable for sludge and straw composting experiments, soil remediation and amendment experiments.

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“…In microbial fermentation, also transport and toxicity effects of both substrates and products must be considered. [42][43][44] The formation of γ-glutamylated compounds occurs through GS-like enzymes, like GMAS or IpuC. [18,22,29,29] The GS-like protein AtdA1 from Acinetobacter sp.…”

Section: Upscaling Of Production Using Fed-batch Fermentation With P ...mentioning

confidence: 99%

γ‐Glutamylation of Isopropylamine by Fermentation

Benninghaus,

Zagami,

Tassini

et al. 2023

ChemBioChem

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Glutamylation yields N‐functionalized amino acids in several natural pathways. γ‐Glutamylated amino acids may exhibit improved properties for their industrial application, e. g., as taste enhancers or in peptide drugs. γ‐Glutamyl−isopropylamide (GIPA) can be synthesized from isopropylamine (IPA) and l‐glutamate. In Pseudomonas sp. strain KIE171, GIPA is an intermediate in the biosynthesis of l‐alaninol (2‐amino‐1‐propanol), a precursor of the fluorochinolone antibiotic levofloxacin and of the chloroacetanilide herbicide metolachlor. In this study, fermentative production of GIPA with metabolically engineered Pseudomonas putida KT2440 using γ‐glutamylmethylamide synthetase (GMAS) from Methylorubrum extorquens was established. Upon addition of IPA during growth with glycerol as carbon source in shake flasks, the recombinant strain produced up to 21.8 mM GIPA. In fed‐batch bioreactor cultivations, GIPA accumulated to a titer of 11 g L−1 with a product yield of 0.11 g g−1 glycerol and a volumetric productivity of 0.24 g L−1 h−1. To the best of our knowledge, this is the first fermentative production of GIPA.

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Whole cell biotransformation for reductive amination reactions

Cited by 15 publications

References 31 publications

Metabolic Engineering of Raoultella ornithinolytica BF60 for Production of 2,5-Furandicarboxylic Acid from 5-Hydroxymethylfurfural

Metabolic Engineering of Raoultella ornithinolytica BF60 for Production of 2,5-Furandicarboxylic Acid from 5-Hydroxymethylfurfural

Compositional changes and ecological characteristics of earthworm mucus under different electrical stimuli

γ‐Glutamylation of Isopropylamine by Fermentation

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