Whole-cell biosensors for environmental monitoring

Rawson, David M.; Willmer, Allison J.; Turner, Anthony

doi:10.1016/0265-928x(89)80011-2

Cited by 158 publications

(71 citation statements)

References 10 publications

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“…Mounted onto electrodes, the cells functioned as the sensor component of the larger detector. In one example, photosynthetic cyanobacteria were used as biocatalysts that reduced an electrochemical mediator, [Fe(CN) 6 ] 3-/4-, which then donated an electron to the electrode, producing a steady current flow that correlated to photosynthetic activity (5). This activity and the ensuing current were disrupted by herbicides and, therefore, could report their presence in tested water.…”

Section: Whole-cell Biosensingmentioning

confidence: 99%

Synthetic biology devices for in vitro and in vivo diagnostics

Slomovic

Pardee

Collins

2015

Proc. Natl. Acad. Sci. U.S.A.

296

228

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There is a growing need to enhance our capabilities in medical and environmental diagnostics. Synthetic biologists have begun to focus their biomolecular engineering approaches toward this goal, offering promising results that could lead to the development of new classes of inexpensive, rapidly deployable diagnostics. Many conventional diagnostics rely on antibody-based platforms that, although exquisitely sensitive, are slow and costly to generate and cannot readily confront rapidly emerging pathogens or be applied to orphan diseases. Synthetic biology, with its rational and short design-to-production cycles, has the potential to overcome many of these limitations. Synthetic biology devices, such as engineered gene circuits, bring new capabilities to molecular diagnostics, expanding the molecular detection palette, creating dynamic sensors, and untethering reactions from laboratory equipment. The field is also beginning to move toward in vivo diagnostics, which could provide near real-time surveillance of multiple pathological conditions. Here, we describe current efforts in synthetic biology, focusing on the translation of promising technologies into pragmatic diagnostic tools and platforms.synthetic biology | diagnostics | biosensing | synthetic gene networks | nanobiotechnology

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Section: Whole-cell Biosensingmentioning

confidence: 99%

Synthetic biology devices for in vitro and in vivo diagnostics

Slomovic

Pardee

Collins

2015

Proc. Natl. Acad. Sci. U.S.A.

296

228

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“…[1][2][3][4][5] The rapid detection methodologies applicable to these fields using CBAs have had enormous impact on the emergence of cellbased biosensors (CBBs). [5][6][7][8] In a CBB, the normal physiological response of a living cell or cellular component resulted by one or more external factors (stimuli) is recorded. 9,10 This 'response' can be qualitatively or quantitatively analyzed to deduce the nature and character of the external 'stimuli' .…”

mentioning

confidence: 99%

“…9,10 This 'response' can be qualitatively or quantitatively analyzed to deduce the nature and character of the external 'stimuli' . 5,6,[8][9][10][11] In some of these CBBs the sensing elements could be the vegetative cells of bacteria, 5,12 or eukaryotic 13 or mammalian cells. 8,14,15 Different types of platforms or materials have been used to 'pattern' or lay different types of higher eukaryotic cells to design cell-based sensors.…”

mentioning

confidence: 99%

“…8,14,15 Different types of platforms or materials have been used to 'pattern' or lay different types of higher eukaryotic cells to design cell-based sensors. 5,10,[16][17][18][19][20] Interfacial engineering to couple biological cell (or cellular components) to signal transducers or detection platforms is critical for successful cell-based biosensing. [21][22][23][24] The types of materials used for immobilization and entrapment of living cells vary widely, encompassing biocompatible polymers (such as polyethylene glycol, alginate, synthetic peptides or silica gel) [25][26][27][28] to biologically derived substances such as collagen or fibronectin, to name a few.…”

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confidence: 99%

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A novel and simple cell-based detection system with a collagen-encapsulated B-lymphocyte cell line as a biosensor for rapid detection of pathogens and toxins

Banerjee

Lenz

Robinson

et al. 2008

Laboratory Investigation

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Cell-based biosensors (CBBs) are becoming important tools for biosecurity applications and rapid diagnostics in food microbiology for their unique capability of detecting physiologically hazardous materials. A multi-well plate-based biosensor containing B-cell hybridoma, Ped-2E9, encapsulated in type I collagen matrix, was developed for rapid detection of viable cells of pathogenic Listeria, the toxin listeriolysin O, and the enterotoxin from Bacillus species. This sensor measures the alkaline phosphatase release from infected Ped-2E9 cells colorimetrically. Pathogenic L. monocytogenes cells and toxin preparations from L. monocytogenes or B. cereus showed cytotoxicity ranging from 24 to 98% at 3-6 h postinfection. In contrast, nonpathogenic L. innocua (F4247) and B. subtilis induced minimal cytotoxicity, ranging only 0.4-7.6%. Laser scanning cytometry and cryo-nano scanning electron microscopy confirmed the live or dead status of the infected Ped-2E9 cells in gel matrix. This paper presents the first example of a cell-based sensing system using collagen-encapsulated mammalian cells for rapid detection of pathogenic bacteria or toxin, and demonstrates a potential for onsite use as a portable detection system.

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“…Biosensors operating in the workplace atmosphere can be used as a warning device so that immediate action can be taken to evacuate the workplace and to locate the source of release. However most biosensors using enzymes, immunoagents or microorganisms are effective in aqueous solutions [3], [4] and [5]. Our aim is to construct a biosensor capable to respond to a toxic volatile organic compound (VOC) such as perchloroethylene present in the form of aerosol.…”

Section: Introductionmentioning

confidence: 99%

Biosensor using immobilized Chlorella microalgae for determination of volatile organic compounds

Naessens

Tran‐Minh

1999

Sensors and Actuators B: Chemical

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A biosensor using Chlorella microalgae immobilized on the membrane of an oxygen electrode has been designed to determine volatile organic compounds (VOC) in the form of aerosols. A homemade controlled atmosphere chamber is constructed for perchloroethylene detection. Monitoring of perchloroethylene is obtained by the measurement of the oxygen production during the algae photosynthetic process. K Ke ey yw wo or rd ds s: :

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Whole-cell biosensors for environmental monitoring

Cited by 158 publications

References 10 publications

Synthetic biology devices for in vitro and in vivo diagnostics

Synthetic biology devices for in vitro and in vivo diagnostics

A novel and simple cell-based detection system with a collagen-encapsulated B-lymphocyte cell line as a biosensor for rapid detection of pathogens and toxins

Biosensor using immobilized Chlorella microalgae for determination of volatile organic compounds

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