Whole-Cell Biosensors as Tools for the Detection of Quorum-Sensing Molecules: Uses in Diagnostics and the Investigation of the Quorum-Sensing Mechanism

O’Connor, Gregory; Knecht, Leslie D.; Strobel, Sebastian; Pasini, Patrizia; Daunert, Sylvia

doi:10.1007/10_2015_337

Cited by 19 publications

(19 citation statements)

References 60 publications

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“…The reporter protein may be detected optically or electrochemically; for example, the measurement of an amount of the protein luciferase encoded by lux gene can be quantified via bioluminescence. QS biosensors serve as fast and reliable tools for detecting specific or various types of autoinductors (Wynendaele et al 2013;O'Connor et al 2015;Rai et al 2015). Examples of methods based on biosensors are listed below (Table 1).To detect AHLs, mutants of bacteria strains that naturally produce bioluminescence in response to AHL molecules may also be used.…”

Section: Measurement Of Ai Productionmentioning

confidence: 99%

“…This strain is also impaired in AHL detection; thus, bioluminescence may only be observed when exogenous AI-2 is present in bacterial environment. Bioluminescence intensity is correlated with an amount of tested autoinductors (O'Connor et al 2015). Genetic modifications could also be used to construct bacterial strains with specific QQ activity.…”

Section: Measurement Of Ai Productionmentioning

confidence: 99%

See 1 more Smart Citation

Prevention of biofilm formation by quorum quenching

Paluch

Rewak‐Soroczyńska

Jędrusik

et al. 2020

Appl Microbiol Biotechnol

286

151

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Quorum sensing (QS) is a mechanism that enables microbial communication. It is based on the constant secretion of signaling molecules to the environment. The main role of QS is the regulation of vital processes in the cell such as virulence factor production or biofilm formation. Due to still growing bacterial resistance to antibiotics that have been overused, it is necessary to search for alternative antimicrobial therapies. One of them is quorum quenching (QQ) that disrupts microbial communication. QQ-driving molecules can decrease or even completely inhibit the production of virulence factors (including biofilm formation). There are few QQ strategies that comprise the use of the structural analogues of QS receptor autoinductors (AI). They may be found in nature or be designed and synthesized via chemical engineering. Many of the characterized QQ molecules are enzymes with the ability to degrade signaling molecules. They can also impede cellular signaling cascades. There are different techniques used for testing QS/QQ, including chromatography-mass spectroscopy, bioluminescence, chemiluminescence, fluorescence, electrochemistry, and colorimetry. They all enable qualitative and quantitative measurements of QS/QQ molecules. This article gathers the information about the mechanisms of QS and QQ, and their effect on microbial biofilm formation. Basic methods used to study QS/QQ, as well as the medical and biotechnological applications of QQ, are also described. Basis research methods are also described as well as medical and biotechnological application.

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Section: Measurement Of Ai Productionmentioning

confidence: 99%

Section: Measurement Of Ai Productionmentioning

confidence: 99%

Prevention of biofilm formation by quorum quenching

Paluch

Rewak‐Soroczyńska

Jędrusik

et al. 2020

Appl Microbiol Biotechnol

286

151

View full text Add to dashboard Cite

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“…Selecting these correctly, genetically modified bacteria can be performed by for example antibiotic resistance genes (O'Connor et al, 2016). …”

Section: Analytical Techniquesmentioning

confidence: 99%

“…Genetic modifications of the BB170 strain ensure it does not produce endogenous autoinducer-1 (AHL's) or autoinducer-2 and is insensitive for autoinducer-1. Hence, bioluminescence only occurs when autoinducer-2 is added, where the light intensity correlates with the amount of autoinducer-2 that is added (O'Connor et al, 2016). …”

Section: Analytical Techniquesmentioning

confidence: 99%

Peptides as Quorum Sensing Molecules: Measurement Techniques and Obtained Levels In vitro and In vivo

et al. 2017

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The expression of certain bacterial genes is regulated in a cell-density dependent way, a phenomenon called quorum sensing. Both Gram-negative and Gram-positive bacteria use this type of communication, though the signal molecules (auto-inducers) used by them differ between both groups: Gram-negative bacteria use predominantly N-acyl homoserine lacton (AHL) molecules (autoinducer-1, AI-1) while Gram-positive bacteria use mainly peptides (autoinducer peptides, AIP or quorum sensing peptides). These quorum sensing molecules are not only involved in the inter-microbial communication, but can also possibly cross-talk directly or indirectly with their host. This review summarizes the currently applied analytical approaches for quorum sensing identification and quantification with additionally summarizing the experimentally found in vivo concentrations of these molecules in humans.

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“…AHLs are amphiphilic chemical compounds that share the same structure including a hydrophilic homoserine lactone ring and a hydrophobic acyl side chain (O'Connor et al, 2015). The diversity of AHLs is based on the number of carbon atoms (4, 6, 7, 8, 10, 12, 14, 16, or 18) on the acyl side chain and substituent (hydrogen, oxhydryl or carbonyl; Kumari et al, 2006) present on the 3rd carbon atom, which are also the basis for distinction and important in regulating specificity.…”

Section: Introductionmentioning

confidence: 99%

Detection of Diverse N-Acyl-Homoserine Lactones in Vibrio alginolyticus and Regulation of Biofilm Formation by N-(3-Oxodecanoyl) Homoserine Lactone In vitro

Liu

Wang

et al. 2017

Front. Microbiol.

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Quorum sensing (QS) is a cell-to-cell communication system based on the exchange of small intercellular signal molecules, such as N-Acyl homoserine lactones (AHLs), which act as cell-density mediators of QS gene expression, and are highly variable both in types and amounts in most Gram-negative Proteobacteria. Understanding the regulation of AHLs may contribute to the elucidation of cell density-dependent phenomena, such as biofilm formation. Vibrio alginolyticus is among the most frequently observed marine opportunistic Vibrio pathogens. However, AHL production of this species and its effects on biofilm formation remain to be understood. Here, our study reported the diverse AHL profiles of 47 marine-isolated V. alginolyticus strains and the effects of exogenous 3-oxo-C10-HSL on biofilm formation under different temperature conditions (16°C and 28°C). A total of 11 detected AHLs were produced by the isolates, of which 3-OH-C4-HSL, 3-oxo-C10-HSL and 3-oxo-C14-HSL comprised the largest proportions. We also observed that moderate levels of exogenous 3-oxo-C10-HSL (10 and 20 μM) could induce or enhance biofilm formation and alter its structure, while high levels (40 and 100 μM) did not significantly improve and even inhibited biofilm formation in V. alginolyticus. Further, regulation by exogenous 3-oxo-C10-HSL was both concentration- and temperature-dependent in V. alginolyticus.

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Whole-Cell Biosensors as Tools for the Detection of Quorum-Sensing Molecules: Uses in Diagnostics and the Investigation of the Quorum-Sensing Mechanism

Cited by 19 publications

References 60 publications

Prevention of biofilm formation by quorum quenching

Prevention of biofilm formation by quorum quenching

Peptides as Quorum Sensing Molecules: Measurement Techniques and Obtained Levels In vitro and In vivo

Detection of Diverse N-Acyl-Homoserine Lactones in Vibrio alginolyticus and Regulation of Biofilm Formation by N-(3-Oxodecanoyl) Homoserine Lactone In vitro

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