Whole-cell biosensor for determination of methanol

Wen, Guangming; Wen, Xiangping; Shuang, Shaomin; Choi, Martin M. F.

doi:10.1016/j.snb.2014.04.107

Cited by 36 publications

(19 citation statements)

References 33 publications

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“…4A). Moreover, the nanosensor in cell-free extract and whole-cells may be in different conformations which has been also mentioned by other research workers in the literature [12,19]. However, as far as the immobilized cell-free extract and wholecells on onion membrane are concerned, the data revealed an increase in the ratio as a function of glucose concentration which suggest that there is an increase in FRET efficiency (Fig.…”

Section: Glucose Assaysupporting

confidence: 68%

“…Although several research workers have used these genetically encoded nanosensors for glucose assay, they were used in a soluble and purified form [10]. In the last decade, there is an increasing interest on whole-cell biosensors [12][13][14] since microbial cells can be genetically manipulated to be used as whole-cell biosensors for a wide range of biomolecules in order to optimize the sensitivity, selectivity and robustness. On the other hand, the nature of immobilization supports used and the high throughput assay method are also critical issues for a good glucose biosensor.…”

Section: Introductionmentioning

confidence: 99%

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Non-enzymatic assay for glucose by using immobilized whole-cells of E. coli containing glucose binding protein fused to fluorescent proteins

Charneca

Karmali

Vieira

2015

Sensors and Actuators B: Chemical

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Genetically encoded nanosensor 3.2 mM High throughput glucose assay Immobilization on 96-well microtiter plates FRET a b s t r a c t Glucose monitoring in vivo is a crucial issue for gaining new understanding of diabetes. Glucose binding protein (GBP) fused to two fluorescent indicator proteins (FLIP) was used in the present study such as FLIP-glu-3.2 mM. Recombinant Escherichia coli whole-cells containing genetically encoded nanosensors as well as cell-free extracts were immobilized either on inner epidermis of onion bulb scale or on 96-well microtiter plates in the presence of glutaraldehyde. Glucose monitoring was carried out by Förster Resonance Energy Transfer (FRET) analysis due the cyano and yellow fluorescent proteins (ECFP and EYFP) immobilized in both these supports.The recovery of these immobilized FLIP nanosensors compared with the free whole-cells and cell-free extract was in the range of 50-90%. Moreover, the data revealed that these FLIP nanosensors can be immobilized in such solid supports with retention of their biological activity. Glucose assay was devised by FRET analysis by using these nanosensors in real samples which detected glucose in the linear range of 0-24 mM with a limit of detection of 0.11 mM glucose. On the other hand, storage and operational stability studies revealed that they are very stable and can be re-used several times (i.e. at least 20 times) without any significant loss of FRET signal. To author's knowledge, this is the first report on the use of such immobilization supports for whole-cells and cell-free extract containing FLIP nanosensor for glucose assay. On the other hand, this is a novel and cheap high throughput method for glucose assay.

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Section: Glucose Assaysupporting

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Non-enzymatic assay for glucose by using immobilized whole-cells of E. coli containing glucose binding protein fused to fluorescent proteins

Charneca

Karmali

Vieira

2015

Sensors and Actuators B: Chemical

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“…Particularly, noble metal-based nanoparticles, such as gold nanoparticles (AuNPs) with large specific surface area and excellent biocompatibility (Wen et al, 2014), Palladium nanoparticles (PdNPs) that possess excellent electrocatalytic performance (Qi et al, 2014), have been extensively used in the construction of biosensors (Wang et al, 2014a;Xia et al, 2013;Lan et al, 2014). Recently, as the result of the enhanced physical, chemical, and prominent catalytic properties over the corresponding monometallic components (He et al, 2012;Ferrer et al, 2007), bimetallic nanoparticles with core-shell structures have aroused much attention in various of biosensors (Gui and Jin, 2013;Miao et al, 2014;Chung et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

An electrochemical aptasensor for thrombin using synergetic catalysis of enzyme and porous Au@Pd core–shell nanostructures for signal amplification

Yuan

et al. 2015

Biosensors and Bioelectronics

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“…Quite a few of these liquids are unsafe for human health and the body. For instance, methyl alcohol is injurious to the human nervous system and can be a cause of severe medical conditions such as coma, blindness or immediate death if it is inhaled [1]. Consequently, the exact quantification or detection of chemical fluids used in many industrial applications is very important.…”

Section: Introductionmentioning

confidence: 99%

Microwave Chemical Sensor Using Substrate-Integrated-Waveguide Cavity

Memon

Lim

2016

Sensors

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This research proposes a substrate-integrated waveguide (SIW) cavity sensor to detect several chemicals using the millimeter-wave frequency range. The frequency response of the presented SIW sensor is switched by filling a very small quantity of chemical inside of the fluidic channel, which also causes a difference in the effective permittivity. The fluidic channel on this structure is either empty or filled with a chemical; when it is empty the structure resonates at 17.08 GHz. There is always a different resonant frequency when any chemical is injected into the fluidic channel. The maximum amount of chemical after injection is held in the center of the SIW structure, which has the maximum magnitude of the electric field distribution. Thus, the objective of sensing chemicals in this research is achieved by perturbing the electric fields of the SIW structure.

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Whole-cell biosensor for determination of methanol

Cited by 36 publications

References 33 publications

Non-enzymatic assay for glucose by using immobilized whole-cells of E. coli containing glucose binding protein fused to fluorescent proteins

Non-enzymatic assay for glucose by using immobilized whole-cells of E. coli containing glucose binding protein fused to fluorescent proteins

An electrochemical aptasensor for thrombin using synergetic catalysis of enzyme and porous Au@Pd core–shell nanostructures for signal amplification

Microwave Chemical Sensor Using Substrate-Integrated-Waveguide Cavity

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