Whole-cell biochips for online water monitoring

Elad, Tal; Belkin, Shimshon

doi:10.4161/bbug.18879

Cited by 6 publications

(5 citation statements)

References 24 publications

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“…Sensors based on enzymatic and whole cell methodologies − have been developed and have shown differing capability to monitor air toxicants. The simplicity of maintaining and sensitivity of bacterial-based biosensors make them worth exploring. ,,− In this study, immobilized bacteria of E. coli TV1061 strain, sensitive to cytotoxic stresses, were exposed to various air toxicants (Figure ) at multiple concentrations (Table ) and these were seen to respond differently. In some cases they were highly induced (ammonium hydroxide or dimethylformamide) or inhibited (formaldehyde or piperidine).…”

Section: Discussionmentioning

confidence: 99%

Bioluminescent Liquid Light Guide Pad Biosensor for Indoor Air Toxicity Monitoring

2015

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Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

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Section: Discussionmentioning

confidence: 99%

Bioluminescent Liquid Light Guide Pad Biosensor for Indoor Air Toxicity Monitoring

2015

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“…By taking advantage of the peculiar features of new biocompatible nanomaterials, hybrid devices with immobilized or micropatterned cells, including bacteria, yeast, or mammalian cells, have been developed and applied to different fields from drug discovery [ 37 ] to environmental monitoring, [ 38–40 ] food control, [ 41 ] and antidoping screening. [ 42 ]…”

Section: Bioluminescence Whole‐cell Biosensorsmentioning

confidence: 99%

Bioluminescence goes portable: recent advances in whole‐cell and cell‐free bioluminescence biosensors

et al. 2020

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Recent advancements in synthetic biology, organic chemistry, and computational models have allowed the application of bioluminescence in several fields, ranging from well established methods for detecting microbial contamination to in vivo imaging to track cancer and stem cells, from cell‐based assays to optogenetics. Moreover, thanks to recent technological progress in miniaturized and sensitive light detectors, such as photodiodes and imaging sensors, it is possible to implement laboratory‐based assays, such as cell‐based and enzymatic assays, into portable analytical devices for point‐of‐care and on‐site applications. This review highlights some recent advances in the development of whole‐cell and cell‐free bioluminescence biosensors with a glance on current challenges and different strategies that have been used to turn bioassays into biosensors with the required analytical performance. Critical issues and unsolved technical problems are also highlighted, to give the reader a taste of this fascinating and challenging field.

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“…In recent decades biological early warning systems [BEWS] using living organisms ranging from bacteria and algae to daphnids, gammarids, and even fish have been developed and tested [4,5,19,30,33,45,76]. Cell-based biosensors rely on oxygen consumption [bacterial respirometers], nitrification, carbon dioxide production, bacterial growth, fluorescence, or photosynthesis [5,14,49].…”

Section: International Journal Of Scientific Research In Environmentamentioning

confidence: 99%

“…Further online biomonitors base on luminescence of bacteria, growth or photosynthesis of algae or behavioural changes of multicellular species like crustacean or fish [5,19,71]. Biological early warning systems [BEWS] have to fulfil several properties: The system must be affordable in acquisition and maintenance, it must respond fast with high accuracy, it should have a broad applicability, and the selected test organism must be sensitive and appropriate for the demands of application and the prevalent conditions.…”

Section: Biomonitoringmentioning

confidence: 99%

Sensitivity Towards Copper: Comparison of Stygal and Surface Water Species’ Biomonitoring Performance in Water Quality Surveillance

Grimm¹,

Gerhardt²

2018

IJSRET

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In a rapidly urbanizing global environment, anthropogenic sources of pollution [e.g., heavy metals, pharmaceuticals] have increasingly contaminated drinking water sources like surface-or groundwater, jeopardizing the safe provision of potable water. Due to the lack of appropriate technology, continuous water surveillance is yet not feasible and while biological early warning systems have shown promising application possibilities, their associated drawbacks limit their practical utility. In this study, two stygal species, Niphargopsis casparyi [Pratz, 1866] and Proasellus slavus [Remy, 1948], were introduced as novel biomonitoring candidates that may reduce maintenance time and cost. Firstly, copper sensitivity of the stygal and two surface species, Gammarus fossarum and Daphnia magna, were defined in acute and chronic toxicity tests. Secondly, using the Multispecies Freshwater Biomonitor [MFB], behavioural responses of all species were examined and compared in multiple copper pulse experiments. Lastly, in a 2-month long field experiment, the applicability of N. casparyi in water quality survey was tested in a local waterworks. Its performance was then compared to G. fossarum and an implemented biomonitor using daphnids. Regarding mortality, copper toxicity tests showed low impact of acute copper pulses onto the two stygal species N. casparyi and P. slavus, but high impact on the surface water species. Chronic exposure led to increased mortality in G. fossarum and P. slavus, but not on N. casparyi. Overall, the pulse and field experiments revealed pronounced, useful, and reproducible behavioural responses towards copper for G. fossarum, N. casparyi, D. magna, but not for P. slavus, making them promising candidates for online biomonitoring.

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Whole-cell biochips for online water monitoring

Cited by 6 publications

References 24 publications

Bioluminescent Liquid Light Guide Pad Biosensor for Indoor Air Toxicity Monitoring

Bioluminescent Liquid Light Guide Pad Biosensor for Indoor Air Toxicity Monitoring

Bioluminescence goes portable: recent advances in whole‐cell and cell‐free bioluminescence biosensors

Sensitivity Towards Copper: Comparison of Stygal and Surface Water Species’ Biomonitoring Performance in Water Quality Surveillance

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