Whole-cell biocatalysis using cytochrome P450 monooxygenases for biotransformation of sustainable bioresources (fatty acids, fatty alkanes, and aromatic amino acids)

Park, Hyuna; Park, Gyuyeon; Jeon, Wooyoung; Ahn, Jungoh; Yang, Yung‐Hun; Choi, Kwon‐Young

doi:10.1016/j.biotechadv.2020.107504

Cited by 50 publications

(28 citation statements)

References 141 publications

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“…Indole C3 carbon has a particularly high reactivity that permits various electrophilic aromatic substitutions. The well‐known indoxyl (3‐hydroxyindole) can be synthesized by introducing a hydroxyl moiety through a monooxygenase enzyme reaction at the indole C3 position 52. Subsequent oxidation of indoxyl leads to the generation of indoxyl radicals, and two molecules of indoxyl radicals are converted to a well‐known blue‐colored indigo dye‐a symmetric dimer‐through a self‐dimerization reaction 53–57.…”

Section: Deamination Of Aromatic Amino Acids and Cosmetic And Pharmaceutical Applicationsmentioning

confidence: 99%

Nitrogen‐Neutral Amino Acids Refinery: Deamination of Amino Acids for Bio‐Alcohol and Ammonia Production

Choi

2021

ChemBioEng Reviews

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Amino acids are a valuable bioresource that contains both the acid form of carbon and the amine group of nitrogen. Amino acids not only constitute proteins and the building blocks for living organisms but also provide a key intermediate for controlling the nitrogen balance in cells. The production of amino acids using microorganisms has, along with the development of biotechnology, created a large market for biochemicals. In parallel, there have been various biochemical production methods using pulmonary proteins or direct amino acids as biomass through enzymatic bioconversion or whole‐cell biotransformation. The deamination of amino acids facilitated the applications of 2‐keto and acrylic acids, in particular, for biofuels of bio alcohol, pharmaceuticals, cosmetics, as building blocks for polymers, and other versatile industrial chemicals. In this review, we report on the application of amino acids derived from biochemicals initiated by deamination reactions using deaminase, ammonia‐lyase, and oxidase enzymes for biotechnology applications.

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Section: Deamination Of Aromatic Amino Acids and Cosmetic And Pharmaceutical Applicationsmentioning

confidence: 99%

Nitrogen‐Neutral Amino Acids Refinery: Deamination of Amino Acids for Bio‐Alcohol and Ammonia Production

Choi

2021

ChemBioEng Reviews

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“…Amino acids are essential nutrients in diets, prerequisite not only for the synthesis of protein but as precursors for synthesis of many bioactive molecules that participate in the regulation of signaling pathways and metabolism in the body [2,3]. Studies have revealed that a balanced essential amino acid (EAA) formulation is highly anabolic and important for muscle repair and recovery [4,5].…”

Section: Introductionmentioning

confidence: 99%

Amino Acid Profiling in Edible Shoots of Some Bamboo Species by Using Rp-Uhplc and Their Therapeutical Aspects

Rana

Chongtham

Bisht

et al. 2021

Asian J Pharm Clin Res

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Objective: This study was conducted to assess the amino acid profiles of edible shoots of five species of bamboos growing in various geographical regions of India. Methods: Qualitative and quantitative estimation of free amino acids was done by Reverse Phase-Ultra High Performance Liquid Chromatography (RP-UHPLC). Separation of amino acids was carried out using Zorbax eclipse plus C18 Reverse Phase (1.8 μm), 50 mm × 2.1 mm column on quaternary UHPLC system using modified gradient elution. Derivatization was done by using auto-sampler Agilent’s 1290 infinity. Data assortment and processing were accomplished by using Open Lab Chromatography Data System EZchrome edition, version A.04.06 (Agilent technologies, USA). Results: In the study, 19 free amino acids were identified, eight essential, five conditionally essential, and six non-essential amino acids (EAA). The principal amino acids were tyrosine (29.41–74.64 μg/mg d.w.) and asparagine (12.81–111.04 μg/mg d.w.). The amount of EAAs ranged from 0.46 to 21.11 μg/mg d.w. For the 1st time, taurine and ornithine are reported in bamboo shoots. The analysis exhibited significant variation (p<0.05) in free amino acids content among the five studied species. Of the five species, Phyllostachys mannii was best compared to the other species in amino acid content. Conclusion: Results showed superior amino acid composition of bamboo shoots that can be utilized to improve nutritional value of functional foods. Amino acids have various health and therapeutic properties can contribute to the basis for further expansion of these bamboos and imminent potential in applications of food and pharmaceuticals.

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“…However, sometimes the cell does not allow overproduction of the protein(s) of interest, in particular, when this interferes with the energy life cycle of the host organism ( Brown and Kornberg, 2004 ; Manganelli, 2007 ), which compromises the production of larger amounts of protein. In addition, product degradation and/or the synthesis of unnecessary by-products, which are the result of the presence of other enzymes of the cell’s metabolic shock can constitute an even bigger problem ( Park et al, 2020 ). Actually, this can be the case during UDP-glucose biosynthesis by means of a whole cell system, as several pathways will utilize it and limit the amount of product for a subsequent down-stream processing.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of the Highly Active UDP-Glucose Pyrophosphorylase From Thermocrispum agreste Provides a Highly Efficient Biocatalyst for the Production of UDP-Glucose

Kumpf

Kowalczykiewicz

Szymańska

et al. 2020

Front. Bioeng. Biotechnol.

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Biocatalysis that produces economically interesting compounds can be carried out by using free enzymes or microbial cells. However, often the cell metabolism does not allow the overproduction or secretion of activated sugars and thus downstream processing of these sugars is complicated. Here enzyme immobilization comes into focus in order to stabilize the enzyme as well as to make the overall process economically feasible. Besides a robust immobilization method, a highly active and stable enzyme is needed to efficiently produce the product of choice. Herein, we report on the identification, gene expression, biochemical characterization as well as immobilization of the uridine-5′-diphosphate-glucose (UDP-glucose) pyrophosphorylase originating from the thermostable soil actinobacterium Thermocrispum agreste DSM 44070 ( Ta GalU). The enzyme immobilization was performed on organically modified mesostructured cellular foams (MCF) via epoxy and amino group to provide a stable and active biocatalyst. The soluble and highly active Ta GalU revealed a V max of 1698 U mg –1 (uridine-5′-triphosphate, UTP) and a K m of 0.15 mM (UTP). The optimum reaction temperature was determined to be 50°C. Ta GalU was stable at this temperature for up to 30 min with a maximum loss of activity of 65%. Interestingly, immobilized Ta GalU was stable at 50°C for at least 120 min without a significant loss of activity, which makes this enzyme an interesting biocatalyst for the production of UDP-glucose.

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Whole-cell biocatalysis using cytochrome P450 monooxygenases for biotransformation of sustainable bioresources (fatty acids, fatty alkanes, and aromatic amino acids)

Cited by 50 publications

References 141 publications

Nitrogen‐Neutral Amino Acids Refinery: Deamination of Amino Acids for Bio‐Alcohol and Ammonia Production

Nitrogen‐Neutral Amino Acids Refinery: Deamination of Amino Acids for Bio‐Alcohol and Ammonia Production

Amino Acid Profiling in Edible Shoots of Some Bamboo Species by Using Rp-Uhplc and Their Therapeutical Aspects

Immobilization of the Highly Active UDP-Glucose Pyrophosphorylase From Thermocrispum agreste Provides a Highly Efficient Biocatalyst for the Production of UDP-Glucose

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