Whole-brain monosynaptic inputs and outputs of leptin receptor b neurons of the nucleus tractus solitarii in mice

Sun, Lu; Zhu, Mengchu; Wang, Meng; Hao, Yinchao; Hao, Yaxin; Jing, Xinyi; Yu, Hongxiao; Shi, Yishuo; Zhang, Xiang; Wang, Sheng; Yuan, Fang; Yuan, Xiang Shan

doi:10.1016/j.brainresbull.2023.110693

Cited by 5 publications

(5 citation statements)

References 56 publications

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“…The current study delineates the synaptic connectomics of NTS PNMT neurons by employing cell‐type‐specific anterograde and retrograde tracing methodologies in a PNMT‐Cre mouse model, in line with previously documented protocols. 30 The data illustrate that NTS PNMT neurons predominantly receive synaptic inputs from the medulla oblongata, especially from the area postrema. In contrast, a relatively minor proportion of inputs originates from other brain regions such as the telencephalon, diencephalon, cerebellum, midbrain, and pons.…”

Section: Discussionmentioning

confidence: 85%

Mapping of afferent and efferent connections of phenylethanolamine N‐methyltransferase‐expressing neurons in the nucleus tractus solitarii

Zhu,

Jun,

Nie

et al. 2024

CNS Neurosci Ther

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ObjectivePhenylethanolamine N‐methyltransferase (PNMT)‐expressing neurons in the nucleus tractus solitarii (NTS) contribute to the regulation of autonomic functions. However, the neural circuits linking these neurons to other brain regions remain unclear. This study aims to investigate the connectivity mechanisms of the PNMT‐expressing neurons in the NTS (NTSPNMT neurons).MethodsThe methodologies employed in this study included a modified rabies virus‐based retrograde neural tracing technique, conventional viral anterograde tracing, and immunohistochemical staining procedures.ResultsA total of 43 upstream nuclei projecting to NTSPNMT neurons were identified, spanning several key brain regions including the medulla oblongata, pons, midbrain, cerebellum, diencephalon, and telencephalon. Notably, dense projections to the NTSPNMT neurons were observed from the central amygdaloid nucleus, paraventricular nucleus of the hypothalamus, area postrema, and the gigantocellular reticular nucleus. In contrast, the ventrolateral medulla, lateral parabrachial nucleus, and lateral hypothalamic area were identified as the primary destinations for axon terminals originating from NTSPNMT neurons. Additionally, reciprocal projections were evident among 21 nuclei, primarily situated within the medulla oblongata.ConclusionOur research findings demonstrate that NTSPNMT neurons form extensive connections with numerous nuclei, emphasizing their essential role in the homeostatic regulation of vital autonomic functions.

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Section: Discussionmentioning

confidence: 85%

Mapping of afferent and efferent connections of phenylethanolamine N‐methyltransferase‐expressing neurons in the nucleus tractus solitarii

Zhu,

Jun,

Nie

et al. 2024

CNS Neurosci Ther

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“…Next, we characterized the outputs of NTS neurons by mapping their axonal terminals, employing a widely-used anterograde viral tracing strategy as reported in previous studies ( Sun et al, 2023 ). To concurrently visualize the axonal projections of NTS Phox2b and NTS GABA neurons, both AAV-EF1α-DIO-EYFP and AAV-EF1α-fDIO-mCherry were injected into the NTS from Phox2b-Flpo::Vgat-Cre mouse mice ( Figure 7A ).…”

Section: Resultsmentioning

confidence: 99%

“…Surgical procedures were conducted in accordance with methodologies outlined in prior studies ( Sun et al, 2023 ). Adult mice underwent anesthesia induced by intraperitoneal administration of pentobarbital sodium at a dosage of 60 μg/g.…”

Section: Methodsmentioning

confidence: 99%

“…Subsequently, Gasparini and colleagues utilized an axonal tracer (cholera toxin b) to provide a brain-wide map of neurons that extend their axons to the caudal NTS ( Gasparini et al, 2020 ). Recently, we characterized whole-brain monosynaptic inputs and outputs of leptin receptor b-expressing neurons within the NTS ( Sun et al, 2023 ), providing a neuroanatomical basis for further research on physiological and pathological functions of these neurons. NTS Phox2b neurons represent the majority of excitatory neurons, including glutamatergic and catecholaminergic, whereas Phox2b was almost absent in GABAergic NTS (NTS GABA ) neurons ( Kang et al, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

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Whole-brain inputs and outputs of Phox2b and GABAergic neurons in the nucleus tractus solitarii

Shao,

Kong,

Tian

et al. 2024

Front. Neurosci.

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The nucleus tractus solitarii (NTS) plays a critical role in the homeostatic regulation of respiration, blood pressure, sodium consumption and metabolic processes. Despite their significance, the circuitry mechanisms facilitating these diverse physiological functions remain incompletely understood. In this study, we present a whole-brain mapping of both the afferent and efferent connections of Phox2b-expressing and GABAergic neurons within the NTS. Our findings reveal that these neuronal populations not only receive monosynaptic inputs primarily from the medulla oblongata, pons, midbrain, supra-midbrain and cortical areas, but also mutually project their axons to these same locales. Moreover, intense monosynaptic inputs are received from the central amygdala, the paraventricular nucleus of the hypothalamus, the parasubthalamic nucleus and the intermediate reticular nucleus, along with brainstem nuclei explicitly engaged in respiratory regulation. In contrast, both neuronal groups extensively innervate brainstem nuclei associated with respiratory functions, although their projections to regions above the midbrain are comparatively limited. These anatomical findings provide a foundational platform for delineating an anatomical framework essential for dissecting the specific functional mechanisms of these circuits.

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“…ARN neurons projects to the other nuclei of the hypothalamus, such as periventricular nucleus, as well as to the other areas of the central nervous system (CNS), as distant as the spinal cord (Bouret et al 2004). ARN is involved in the multiple biological functions, such as feeding (Garza et al 2023;Ullah et al 2023), sexual activity (Cortes et al 2022;Zhou et al 2022), and the central regulation of the cardiovascular system (Pan et al 2023;Sun et al 2023).…”

Section: Introductionmentioning

confidence: 99%

Putative identification of proopiomelanocortin and neuropeptide-Y neurons of the arcuate nucleus by their response to leptin: in vivo electrophysiology study in male and female rats

Grinchii,

Kominkova,

Dremencov

2024

gpb

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The arcuate nucleus (ARN) of the hypothalamus is involved in multiple biological functions, such as feeding, sexual activity, and the regulation of the cardiovascular system. It was reported that leptin increased c-Fos expression in the proopiomelanocortin (POMC)-and decreased it in the neuropeptide-Y (NPY)-positive neurons of the ARN, suggesting that it stimulates the former, and inhibits the later. This study aimed at the direct electrophysiological examination of the effect of leptin on ARN neurons and to investigate potential sex-dimorphic changes. Wistar rats were anesthetized with urethane and the electrodes were inserted into the ARN. After a spontaneous active neuron was recorded for at least one minute, leptin was administered intravenously, and the firing activity of the same neuron was recorded for two additional minutes. It was found that approximately half of the ARN neurons had an excitatory, and another half an inhibitory response to the leptin administration. The excitability of the neurons with excitatory response to leptin was not different between the sexes. The average firing rate of the neurons with inhibitory response to leptin in females was, however, significantly lower comparing to the males. The obtained results demonstrate that the ARN neurons with stimulatory response to leptin are POMC and those with inhibitory response are NPY neurons. NPY Y1 receptor be might responsible, at least in part, for the sex differences in the excitability of the neurons putatively identified as NPY neurons.

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Whole-brain monosynaptic inputs and outputs of leptin receptor b neurons of the nucleus tractus solitarii in mice

Cited by 5 publications

References 56 publications

Mapping of afferent and efferent connections of phenylethanolamine N‐methyltransferase‐expressing neurons in the nucleus tractus solitarii

Mapping of afferent and efferent connections of phenylethanolamine N‐methyltransferase‐expressing neurons in the nucleus tractus solitarii

Whole-brain inputs and outputs of Phox2b and GABAergic neurons in the nucleus tractus solitarii

Putative identification of proopiomelanocortin and neuropeptide-Y neurons of the arcuate nucleus by their response to leptin: in vivo electrophysiology study in male and female rats

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