Whole brain mapping of somatosensory responses in awake marmosets investigated with ultra-high field fMRI

Abstract

The common marmoset (Callithrix jacchus) is a small-bodied New World primate that is becoming an important model to study brain functions. Despite several studies exploring the somatosensory system of marmosets, all results have come from anesthetized animals using invasive techniques and post-mortem analyses. Here we demonstrate the feasibility for getting high-quality and reproduceable sensorimotor mapping in awake marmosets with functional magnetic resonance imaging (fMRI). We acquired fMRI sequences in four animals while they received tactile stimulation (via air-puffs), delivered to the face, arm or leg. We found that the body representation progressed medially from the leg to the face in areas 3a, 3b, 1/2, and from caudal to rostral sites in areas S2 and PV. SI and SII exhibited a body representation in their functional connectivity pattern within the posterior and midcingulate and the thalamus. Interestingly, we also found a somatotopic body representation in two subcortical areas: the thalamus and, for the first time, in the putamen. These maps have similar organizations as those previously found in Old World macaque monkeys and humans, suggesting that these subcortical somatotopic organizations were already established before Old and New World primates diverged. Our results show the first whole brain mapping of somatosensory responses acquired in a non-invasive way in awake marmosets.Significant statementHere we used somatosensory stimulation combined with functional magnetic resonance imaging to map whole brain activation in awake marmosets. We used light tactile stimulation, consisting of air-puffs, delivered on the face, arm or leg. We found a topographic body representation in primary (SI) and secondary (SII) somatosensory regions, thalamus and putamen. We also revealed the existence of a body representation organization within the thalamus and the cingulate cortex by computing functional connectivity maps from seeds defined in SI/SII for face, arm and leg using resting-state fMRI data. This non-invasive approach will be essential for chronic studies by guiding invasive recording and manipulation techniques.

Interspecies activation correlations reveal functional correspondences between marmoset and human brain areas

“…Task-based fMRI is better suited for mapping stimuli-driven fluctuations across species and indeed a few studies have used task-based fMRI in awake marmosets to identify areas related to specific functions (e.g. visuo-saccadic orienting (Schaeffer et al, 2019d), processing of faces and bodies (Hung et al, 2015; Schaeffer et al, 2020b), looming and receding visual stimuli (Cléry et al, 2020, Neuroimage), and tactile processing (Clery et al, 2020). A major drawback of task-based fMRI is that compliance is often poor in NHPs and each task can only reveal the limited set of functional activations for which it was designed.…”