Whole-body protein turnover in humans fed a soy protein-rich vegetable diet

Abstract

Objectives: This study was designed to compare the whole-body protein turnover in humans after the ingestion of a soy protein-rich vegetable diet with that of a control group fed a western animal protein-rich diet. Subjects: Twelve male volunteers were divided into two groups of six subjects who were given for two weeks either a 85% vegetable protein diet (diet VP) or a control western animal protein-rich diet (diet AP). Interventions: Whole-body protein turnover was estimated at the end of the two-week controlled diet period using the [15 N]-glycine end-product method. Nitrogen¯ux rates were determined in the fed state (1.3 g protein/ kg) over a 9 h period after the dose of [ 15 N]-glycine was given. Results: After the 9 h of the test, the urinary ammonia excretion was signi®cantly higher in the group receiving the diet AP than that in the group receiving the diet VP (P`0.05). In contrast, there was no signi®cant difference for both total nitrogen and urea nitrogen excretions. Both the protein synthesis and the protein breakdown were similar in both groups. In the same way, the net protein deposition measured in the fed state during 9 h was similar for both diets at 0.07 g/kg/h. Conclusions: Young adults fed 1.3 g/kg/d of either meat or vegetable protein-rich diet for two weeks did not show a different protein turnover. Sponsorship: This work was supported in part by grant from Eridania Be Âghin-Say Compagny.

The end-product method of measuring whole-body protein turnover: a review of published results and a comparison with those obtained by leucine infusion

“…{ References for results by the precursor method will be presented in a forthcoming book (Waterlow, In Press). Marchini et al (1996); 2, Soares et al (1991);3, Jackson et al (2000); 4, Gausseres et al (1997); 5, Grove & Jackson (1995);6, McNurlan et al (1987);7, Fern et al (1985b);8, Fern et al (1984); 9, ; 10, Fern et al (1981);11, Soares et al (1994); 12, Acheson et al (1995); 13, Robinson et al (1990); 14, Willommet et al (1992); 15, Stein et al (1996);16, Stein et al (1990); 17, Conway et al method may be the one of choice for population studies aimed at determining whole-body rates of protein synthesis and their functional correlates in genetically or environmentally different groups. We hoped also that the flux ratio would give interesting information about the partition of protein metabolism between peripheral and visceral tissues.…”

“…References: 1,Stein et al (1996); 2,Marchini et al (1996); 3,Soares et al (1991); 4, Jackson et al (2000); 5,Gausseres et al (1997); 6,Grove & Jackson (1995);7, Willommet et al (1992); 8, McNurlan et al (1987); 9, Fern et al (1984); 10, Fern & Garlick (1983); 11, Fern et al (1981); 12, Swart et al (1988); 13, Soares et al (1994); 14, Acheson et al (1995); 15, Robinson et al (1990); 16, Stein et al (1990); 17, Fern et al (1985b); 18, Conway et al (1995); 19, Pannemans et al (1995); 20, Zillikens et al (1993); 21, Bos et al (2000); 22, Badaloo et al (1989); 23, de Benoist et al (1985); 24, Jackson et al (1997); 25, Jackson et al (1987); 26, Pacy et al (1994); 27, Bettany et al (1996); 28, Ang et al (1995); 29, Nissim et al (1983); 30, Jeevanandam et al (1986); 31, Ma & Jiang (1990); 32, Richards et al (1993); 33, Tracey et al (1988); 34, Jeevanandam et al (1987).…”

Reducing the Dietary Acid Load: How a More Alkaline Diet Benefits Patients With Chronic Kidney Disease

“…31 If soya protein replaces meat protein, ammonium excretion falls significantly (no data on TA). 32 When large doses of sodium bicarbonate (150 mEq/day) are given, this neutralizes dietary acid resulting in minimal ammonium and TA excretion and increased bicarbonate excretion. 33…”

Kidney Response to the Spectrum of Diet-Induced Acid Stress

“…Foods with high base-producing potential include many fruits and vegetables, including raisins, cruciferous vegetables (e.g., broccoli, brussel sprouts, cabbage), leafy greens (kale, spinach, lettuce, collard greens), and soy protein [ 8 ]. Substituting base-producing for acid-producing foods is a particularly effective strategy for dietary H + reduction [ 9 ].…”