Whole body distribution of deuterated linoleic and α-linolenic acids and their metabolites in the rat

Lin, Yu Hong; Salem, Norman

doi:10.1194/jlr.m700369-jlr200

Cited by 72 publications

(55 citation statements)

References 51 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Essential FAs are transported to the BBB either as FFAs bound to albumin or carried with lipoproteins in esterified forms (i.e., PLs and triglycerides) that enter the endothelial cells through the luminal side and are liberated by lipoprotein lipases (65)(66)(67)(68)(69). It is suggested that a number of mechanisms on the abluminal side, such as a flip-flop mechanism and transporters that are specific for essential FA, particularly DHA, ultimately facilitate their entry into the brain (67,70).…”

Section: Discussionmentioning

confidence: 99%

Lipidomic analyses identify injury‐specific phospholipid changes 3 mo after traumatic brain injury

et al. 2014

View full text Add to dashboard Cite

Phospholipid (PL) abnormalities are observed in the cerebrospinal fluid of patients with traumatic brain injury (TBI), suggesting their role in TBI pathology. Therefore, PL levels were examined in a TBI mouse model that received 1.8 mm deep controlled cortical impact injury or craniectomy only (control). The rotarod and Barnes maze acquisition and probe tests were performed within 2 wk after injury, with another probe test performed 3 mo postinjury. Liquid chromatography/mass spectrometry analyses were performed on lipid extracts from several brain regions and plasma from injured and control mice collected at 3 mo postinjury. Compared to controls, injured mice with sensorimotor and learning deficits had decreased levels of cortical and cerebellar phosphatidylcholine (PC) and phosphatidylethanolamine (PE) levels, while hippocampal PC, sphingomyelin and PE levels were elevated. Ether PE levels were lower in the cortices and plasma of injured animals. Polyunsaturated fatty acid-containing PC and PE species, particularly ratios of docosahexaenoic acid (DHA) to arachidonic acid, were lower in the hippocampi and cortices and plasma of injured mice. Given the importance of DHA in maintaining neuronal function and resolving inflammation and of peroxisomes in synthesis of ether PLs, normalizing these PLs may be a useful strategy for treating the chronic pathology of TBI.-Abdullah, L., Evans, J. E., Ferguson, S., Mouzon, B., Montague, H., Reed, J., Crynen, G., Emmerich, T., Crocker, M., Pelot, R., Mullan, M., Crawford, F. Lipidomic analyses identify injury-specific phospholipid changes 3 months after traumatic brain injury. FASEB J. 28, 5311-5321 (2014). www.fasebj.org Key Words: controlled cortical impact ⅐ mass spectrometry ⅐ phosphatidylcholine ⅐ phosphatidylethanolamine ⅐ phosphatidylinositol ⅐ sphingomyelin Traumatic brain injury (TBI) is a significant cause of morbidity and mortality in the United States among adults from both the civilian and military populations. Due to the improvements made in military body armor and combat helmets over the past 2 decades, the rate of severe TBI associated with Iraq and Afghanistan deployments has decreased to 1% of the total TBI sustained by military personnel, a rate that is lower than those reported for previous deployments (1-3). Estimates from the U.S. Centers for Disease Control and Prevention suggest that severe TBI remains an important issue in the civilian population. In 2010, 2.5 million admissions to emergency departments were due to TBI (4) contributing to about a third of injury related deaths in the United States. The annual U.S. costs associated with TBI are estimated to be $75 billion, largely attributed to medical care, loss of productivity, and long-term disability (5). Although mild TBI occurs more frequently than severe TBI, the latter has the largest adverse effect on the activities of daily living of injured individuals and accounts for 90% of the total TBI-related medical costs (5). The primary injury is caused by the immediate force of the trauma a...

show abstract

Section: Discussionmentioning

confidence: 99%

Lipidomic analyses identify injury‐specific phospholipid changes 3 mo after traumatic brain injury

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Conversely, administration of LA results in a short-lived enrichment of LA in brain tissue along with a long-term accumulation of AA [13]. Dietary DHA has been reported to result in DHA enrichment of neuronal plasma membranes [14], although no association between dietary DHA and brain DHA has also been reported in aged animals [15].…”

Section: Dietary Pufasmentioning

confidence: 96%

Dietary polyunsaturated fatty acids improve cholinergic transmission in the aged brain

2009

View full text Add to dashboard Cite

The cholinergic theory of aging states that dysfunction of cholinergic neurons arising from the basal forebrain and terminating in the cortex and hippocampus may be involved in the cognitive decline that occurs during aging and Alzheimer's disease. Despite years of research, pharmacological interventions to treat or forestall the development of Alzheimer's disease have primarily focused on enhancing cholinergic transmission, either through increasing acetylcholine (ACh) synthesis or inhibition of the acetylcholinesterase enzyme responsible for ACh hydrolysis. However, recent studies have indicated that dietary supplementation can impact the cholinergic system, particularly during aging. The purpose of the present review is to examine the relevant research suggesting that cholinergic functioning may be maintained during aging via consuming a diet containing polyunsaturated fatty acids (PUFAs). The data reviewed herein indicate that, at least in animal studies, inclusion of PUFAs in the diet can improve cholinergic transmission in the brain, possibly leading to improvements in cognitive functioning.

show abstract

“…Since deuterated compounds have been shown to exhibit comparable transport and uptake as their non-deuterated counterparts 27,28 this technique has great potential as a suitable 'label' for studying transport kinetics 29,30 . We applied deuterium labeling to the widely used herbicide glyphosate.…”

Section: Agrochemical Imagingmentioning

confidence: 99%

Label-free Chemically Specific Imaging in Planta with Stimulated Raman Scattering Microscopy

Mansfield

Littlejohn²,

Seymour³

et al. 2013

Anal. Chem.

View full text Add to dashboard Cite

The growing world population puts ever-increasing demands on the agricultural and agrochemical industries to increase agricultural yields. This can only be achieved by investing in fundamental plant and agrochemical research and in the development of improved analytical tools to support research in these areas.There is currently a lack of analytical tools that provide non-invasive structural and chemical analysis of plant tissues at the cellular scale. Imaging techniques such as coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS) microscopy provide labelfree chemically specific image contrast based on vibrational spectroscopy. Over the past decade these techniques have been shown to offer clear advantages for a vast range of biomedical research applications. The intrinsic vibrational contrast provides label-free quantitative functional analysis; it does not suffer from photobleaching; and allows near realtime imaging in 3D with submicron spatial resolution. However, due to the susceptibility of current detection schemes to optical absorption and fluorescence from pigments (such as chlorophyll) the plant science and agrochemical research communities have not been able to benefit from these techniques and their application in plant research has remained virtually unexplored.In this paper we explore the effect of chlorophyll fluorescence and absorption in CARS and SRS microscopy. We show that with the latter it is possible to use phase-sensitive detection to separate the vibrational signal from the (electronic) absorption processes. Finally we demonstrate the potential of SRS for a range of in planta applications by presenting in-situ chemical analysis of plant cell wall components, epicuticular waxes, and the deposition of agrochemical formulations onto the leaf surface.

show abstract

Whole body distribution of deuterated linoleic and α-linolenic acids and their metabolites in the rat

Cited by 72 publications

References 51 publications

Lipidomic analyses identify injury‐specific phospholipid changes 3 mo after traumatic brain injury

Lipidomic analyses identify injury‐specific phospholipid changes 3 mo after traumatic brain injury

Dietary polyunsaturated fatty acids improve cholinergic transmission in the aged brain

Label-free Chemically Specific Imaging in Planta with Stimulated Raman Scattering Microscopy

Contact Info

Product

Resources

About