Whole-body cortisol response of zebrafish to acute net handling stress

Ramsay, Jennifer M.; Feist, Grant W.; Varga, Zoltán; Westerfield, Monte; Kent, Michael L.; Schreck, Carl B.

doi:10.1016/j.aquaculture.2009.08.035

Cited by 218 publications

(157 citation statements)

References 52 publications

(78 reference statements)

Supporting

Mentioning

146

Contrasting

Unclassified

Order By: Relevance

“…The area under the curve (reflecting the total amount of cortisol) showed similar low values in proactive fish but a twofold increase in singularly stressed and a threefold increase in repeatedly stressed reactive fish. The full-body baseline and control cortisol levels presented here are consistent with previous studies on stress response in zebrafish, as were peak levels after 15 min (Ramsay et al 2006(Ramsay et al , 2010. This consistency in peak cortisol levels suggests a general physiological response to strong physical stressors independent of coping style, while only after repeated exposure to stressors significant variation in cortisol levels emerges that is strongly dependent on coping style.…”

Section: Discussionsupporting

confidence: 79%

See 1 more Smart Citation

Covariation between behaviour and physiology indicators of coping style in zebrafish (Danio rerio)

Tudorache¹,

Schaaf²,

Slabbekoorn³

2013

Journal of Endocrinology

View full text Add to dashboard Cite

All vertebrates exhibit physiological responses to a wide variety of stressors. The amplitude and profile of the response depend on the intensity, duration, controllability and predictability of the stressor, but there is also individual variation in the response, termed coping style. A better understanding of the expression of coping styles is of great value for medical applications, animal welfare issues and conservation. Here, we investigated the effect of repeated netting stress on proactive and reactive zebrafish (Danio rerio) as an upcoming model system for stress research. Fish were separated by coping styles according to the order of entering a novel environment. Subsequently, repeated netting stress was applied as stressor, over a period of 21 days. Full-body cortisol levels were determined at 0, 15, 30, 60 and 120 min after the last repeated stress event. Our results show that reactive fish display i) increased basal cortisol concentrations after being repeatedly stressed, ii) higher cortisol secretion over time and iii) slow recovery of cortisol concentration towards basal levels after the last repeated stress event. This study shows for the first time in zebrafish that different coping styles are associated with different cortisol responses during the recovery from stress over time and that coping styles can explain otherwise unaccounted variation in physiological stress responses.

show abstract

Section: Discussionsupporting

confidence: 79%

“…We applied a netting stressor according to Ramsay et al (2010). Briefly, fish were gently removed from the holding tank using a hand net (11!24 cm).…”

Section: Stress Proceduresmentioning

confidence: 99%

Covariation between behaviour and physiology indicators of coping style in zebrafish (Danio rerio)

Tudorache¹,

Schaaf²,

Slabbekoorn³

2013

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…The swimming speeds (and the velocity increments) used to test the fish were -according to their size -the following: 30, 32.5 and 35 mm sec −1 . The TL of the fish was assessed visually before the trial to avoid excess stress from the unnecessary fish handling (Ramsay et al 2009). If the actual TL (as measured at the end of the experiments on the digital photographs of the anaesthetized fish) differed from the estimated one and therefore from the applied speed and the velocity increment more than 1.25 mm, then the data concerning that fish were discarded.…”

Section: Methodsmentioning

confidence: 99%

Effect of developmental temperature on swimming performance of zebrafish (Danio rerio) juveniles

2010

View full text Add to dashboard Cite

It is widely known that water temperature affects the swimming capacity of fish. But the effect of the rearing temperature on the swimming ability of the fish at later stages, has not had similar attention. In this study, four populations of zebrafish, were reared in different water temperatures (22, 25, 28 and 31°C) and after being acclimatized in a common temperature (26.5°C) for over a month, they were subjected to swimming trials in order to evaluate the maximum relative critical velocity (RU crit ) in each case. Fish that were reared in 22°C showed statistically significant lower performance than the ones reared in 31°C (7.72 ±0.17 vs. 8.79±0.28, means ± S.E.). Possible explanations for the observed differentiation could be the effect of early life temperature on fish muscle ontogeny or on body shape.

show abstract

“…However, to the best of our knowledge no study has ever addressed how far individual differences in resting metabolic rate reflect intra-specific coping styles. Housing in respirometry chambers implies handling and exposure to a new environment, both conditions known to be a source of stress in a variety of fish species (Martins et al, 2006;Höglund et al, 2007;Ramsay et al, 2009;Silva et al, 2010). As a consequence some individuals may exhibit a fight or flight reaction while others a freezelike reaction.…”

Section: Introductionmentioning

confidence: 99%

Individual differences in metabolism predict coping styles in fish

Martins

Castanheira

Engrola

et al. 2011

Applied Animal Behaviour Science

View full text Add to dashboard Cite

a b s t r a c tStudies on metabolism usually rely on measurements of oxygen consumption obtained in respirometry chambers. Despite rigorous standardization there is still considerable interindividual variation in metabolic rates which is often ignored. Furthermore, housing in respirometry chambers implies handling and exposure to a new environment, conditions known to be a source of stress in animals. The extent of stress response is known to be dependent on an individual's coping style. Therefore, the extent of individual variation in respirometry data may be related to the expression of coping styles. The goal of this study is to determine whether the variation in oxygen consumption after a stressor is the resultant of an individual's coping style. Our approach was to determine oxygen consumption in fish immediately after transfer to respiratory chambers and during the subsequent 22 h. In addition, the same individuals were subjected to 3 respirometry runs (22 h each) to address the question whether habituation occurs when fish are used in multiple runs and whether habituation potential is linked to coping styles. The same individuals were then subjected to an acute stressor and the escape behaviour and plasma cortisol were determined to assess coping style. Taken as a whole, the findings of this study suggest that individual variation in oxygen consumption in respirometry chambers are related to the expression of coping styles: individuals with higher oxygen consumption at the start and throughout the measurement period were shown to react passively (longer latency to escape) in a confinement stress. In addition, successive respirometry runs should not be assumed to result in habituation, as depending on an individual's coping style either habituation (in the case of passive copers) or sensitization (in the case of active copers) can occur.

show abstract

Whole-body cortisol response of zebrafish to acute net handling stress

Cited by 218 publications

References 52 publications

Covariation between behaviour and physiology indicators of coping style in zebrafish (Danio rerio)

Covariation between behaviour and physiology indicators of coping style in zebrafish (Danio rerio)

Effect of developmental temperature on swimming performance of zebrafish (Danio rerio) juveniles

Individual differences in metabolism predict coping styles in fish

Contact Info

Product

Resources

About