Whole blood microsampling for the quantitation of estetrol without derivatization by liquid chromatography-tandem mass spectrometry

Nys, Gwenaël; Gallez, Anne; Kok, Miranda; Cobraiville, Gaël; Servais, Anne-Catherine; Piel, Géraldine; Péqueux, Christel; Fillet, Marianne

doi:10.1016/j.jpba.2017.02.060

Cited by 34 publications

(17 citation statements)

References 39 publications

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“…With improvements in molecular analysis techniques, the demand for sample size in disease diagnosis has significantly reduced. In the case of blood, a microsample generally refers to a sample size of <100 μL as opposed to >1 mL in phlebotomy (Nys et al 2017). A smaller sample demand addresses the limitations in conventional techniques by expanding the potential for a quicker, cost-effective and less invasive diagnostic procedure (Table 1).…”

Section: Blood Samplingmentioning

confidence: 99%

A review of microsampling techniques and their social impact

Lei

Prow

2019

Biomed Microdevices

102

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Conventional skin and blood sampling techniques for disease diagnosis, though effective, are often highly invasive and some even suffer from variations in analysis. With the improvements in molecular detection, the amount of starting sample quantity needed has significantly reduced in some diagnostic procedures, and this has led to an increased interest in microsampling techniques for disease biomarker detection. The miniaturization of sampling platforms driven by microsampling has the potential to shift disease diagnosis and monitoring closer to the point of care. The faster turnaround time for actionable results has improved patient care. The variations in sample quantification and analysis remain a challenge in the microsampling field. The future of microsampling looks promising. Emerging techniques are being clinically tested and monitored by regulatory bodies. This process is leading to safer and more reliable diagnostic platforms. This review discusses the advantages and disadvantages of current skin and blood microsampling techniques.

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Section: Blood Samplingmentioning

confidence: 99%

A review of microsampling techniques and their social impact

Lei

Prow

2019

Biomed Microdevices

102

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“…A wide spectrum of analytes has been successfully determined from VAMS-based samples. For pharmacokinetic studies, estetrol from whole blood VAMS samples obtained from mice was efficiently assessed [18]. Itraconazole was analyzed from VAMS as low soluble molecule model [19].…”

Section: Introductionmentioning

confidence: 99%

Determination of iohexol by capillary blood microsampling and UHPLC-MS/MS

Ion

Legoff

Cavalier

et al. 2019

Journal of Pharmaceutical Analysis

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One of the most important tools used to evaluate kidney function in the context of chronic kidney disease or other renal function related pathologies is the exploration of glomerular filtration rate (GFR). Iohexol is up to this moment a good candidate molecule for the GFR assessment since it exhibits minimum protein binding rates and minimum extra-renal clearance, being neither secreted nor reabsorbed at the tubular level. This study proposes and evaluates a new LC-MS/MS method for the iohexol determination from capillary blood, prelevated using volumetric absorbative microsampling (VAMS) systems. As an alternative to VAMS, a brand new HemaPEN ® device for micro-prelevation was also tested. A new high throughput sample preparation protocol adapted for iohexol quantification from whole blood VAMS samples was developed. The medium term stability study of iohexol in dried whole blood VAMS samples that was conducted showed a good stability of this molecule for up to 12 days. By collecting only 10 μL of blood, iohexol can be analyzed from dried whole blood VAMS samples for concentration ranges between 1 and 250 μg/mL. Due to the analyte stability in VAMS for up to 12 days, this approach might be successfully applied for GFR assessment for clinical cases allowing minimum invasiveness and even delayed analysis.

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“…Circulating levels of E4 in mice were measured as previously described [35]. Plasmatic concentrations of E2 and P4 were measured in the laboratory of Medical Chemistry and Clinical Study of the CHU of Liège.…”

Section: Blood Sampling and Circulating E2 E4 And P4 Quantitationmentioning

confidence: 99%

Estetrol Combined to Progestogen for Menopause or Contraception Indication Is Neutral on Breast Cancer

Gallez

Blacher

Maquoi

et al. 2021

Cancers

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Given the unequivocal benefits of menopause hormone therapies (MHT) and combined oral contraceptives (COC), there is a clinical need for new formulations devoid of any risk of breast cancer promotion. Accumulating data from preclinical and clinical studies support that estetrol (E4) is a promising natural estrogen for MHT and COC. Nevertheless, we report here that E4 remains active on the endometrium, even under a dose that is neutral on breast cancer growth and lung metastasis dissemination. This implies that a progestogen should be combined with E4 to protect the endometrium of non-hysterectomized women from hyperplasia and cancer. Through in vivo observations and transcriptomic analyses, our work provides evidence that combining a progestogen to E4 is neutral on breast cancer growth and dissemination, with very limited transcriptional impact. The assessment of breast cancer risk in patients during the development of new MHT or COC is not possible given the requirement of long-term studies in large populations. This translational preclinical research provides new evidence that a therapeutic dose of E4 for MHT or COC, combined with progesterone or drospirenone, may provide a better benefit/risk profile towards breast cancer risk compared to hormonal treatments currently available for patients.

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Whole blood microsampling for the quantitation of estetrol without derivatization by liquid chromatography-tandem mass spectrometry

Cited by 34 publications

References 39 publications

A review of microsampling techniques and their social impact

A review of microsampling techniques and their social impact

Determination of iohexol by capillary blood microsampling and UHPLC-MS/MS

Estetrol Combined to Progestogen for Menopause or Contraception Indication Is Neutral on Breast Cancer

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