Whole Blood Cells Loaded with Messenger RNA as an Anti‐Tumor Vaccine

Phua, Kyle K.L.; Boczkowski, David; Dannull, Jens; Pruitt, Scott K.; Leong, Kam W.; Nair, Smita K.

doi:10.1002/adhm.201300512

Cited by 39 publications

(29 citation statements)

References 29 publications

(45 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our results suggest that monocytes are the most potent cell type for this purpose. Previous studies have examined the induction of CTL responses by lymphocytes, whole blood cells, and total splenocytes (21,67,68). In the best of these cases, more than 10 7 cells are required to generate antitumor CTL responses equivalent to those induced by DC vaccines (21,67).…”

Section: Methodsmentioning

confidence: 99%

Antigen-loaded monocyte administration induces potent therapeutic antitumor T cell responses

Huang¹,

Nicholson²,

Batich³

et al. 2020

Journal of Clinical Investigation

Self Cite

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Antigen-loaded monocyte administration induces potent therapeutic antitumor T cell responses

Huang¹,

Nicholson²,

Batich³

et al. 2020

Journal of Clinical Investigation

Self Cite

View full text Add to dashboard Cite

“…However, this method may disrupt the cell membrane and result in a partial and irreversible deterioration of the structural integrity 43 . Phosphatidylserine (PS), a signal to mononuclear-phagocyte system for uptake, was found to be externalized after electroporation treatment 41 . This would result in the reticuloendothelial system (RES) phagocytosis and greatly shorten the blood circulation time.…”

Section: Whole Cell As Drug Carriermentioning

confidence: 99%

Cell or Cell Membrane-Based Drug Delivery Systems

et al. 2015

View full text Add to dashboard Cite

Natural cells have been explored as drug carriers for a long period. They have received growing interest as a promising drug delivery system (DDS) until recently along with the development of biology and medical science. The synthetic materials, either organic or inorganic, are found to be with more or less immunogenicity and/or toxicity. The cells and extracellular vesicles (EVs), are endogenous and thought to be much safer and friendlier. Furthermore, in view of their host attributes, they may achieve different biological effects and/or targeting specificity, which can meet the needs of personalized medicine as the next generation of DDS. In this review, we summarized the recent progress in cell or cell membrane-based DDS and their fabrication processes, unique properties and applications, including the whole cells, EVs and cell membrane coated nanoparticles. We expect the continuing development of this cell or cell membrane-based DDS will promote their clinic applications.

show abstract

“…This is a cost, labor and resource intensive procedure. Because of these reasons, researchers have explored alternative cell-based approaches 10,11 , as well as direct in vivo injection of mRNA in naked 12,13 and nanoparticle formats [14][15][16][17][18][19] . However, due to rapid degradation of naked mRNA in vivo, direct injection of mRNA is effective only when it is injected directly into lymph nodes 12,13 .…”

mentioning

confidence: 99%