Whole-bacterial cell enzyme-linked immunosorbent assay for Streptococcus sanguis fimbrial antigens

Abstract

A whole-bacterial cell enzyme-linked immunosorbent assay (bactELISA) was developed for detecting fimbrial antigens on Streptococcus sanguis. In this assay, S. sanguis cells were directly adhered to polystyrene or polyvinyl via drying. Use of the assay indicated that consistently high and uniform optical densities could be obtained from well to well. In addition, radioactive assaying indicated increased adsorbance to the polystyrene wells over polyvinyl, suggesting that polystyrene may prove superior in the gram-positive bactELISA. Use of the bactELISA may prove valuable to both the clinical and research laboratory involved in the study of bacterial cell surface components or in the evaluation of antisera directed against bacterial antigens, which are difficult to prepare as purified derivatives.

Isolation and characterization of Fap1, a fimbriae‐associated adhesin ofStreptococcus parasanguisFW213

“…Surface‐bound Fap1 was determined using a BactELISA (Elder et al ., 1982). Bacteria (1 × 10 8 ) in 50 mM sodium carbonate, pH 9.6, were dried on to the bottom of wells of 96‐well microtitre plates by incubation at 37°C overnight.…”

“…No significant variation in the number of bacteria bound to the wells was detected amongst different strains of S . parasanguis (Elder et al ., 1982). The data were the means from two separate experiments in eight replicates.…”

Characteristics of adherence of Actinobacillus actinomycetemcomitans to epithelial cells

“…Preparation of rabbit antiserum. SUNY 465 cells were harvested during exponential growth, treated with formalin, and injected into New Zealand White adult rabbits in accordance with established protocols (2). The titer in serum was measured by an enzyme-linked immunosorbent assay (2), and high-titer serum was pooled, treated at 56°C for 30 min to destroy complement, and stored at -20°C.…”

Identification of dipeptide repeats and a cell wall sorting signal in the fimbriae‐associated adhesin, Fap1, of Streptococcus parasanguis

“…Surface-bound Fap1 was determined for S. parasanguis using a bactELISA (Elder et al, 1982). No significant variation in the number of bacteria bound to the wells was detected among different strains of S. parasanguis.…”