Who should be screened for chromosomal abnormalities before ICSI treatment?

Dul, E. C.; Ravenswaaij-Arts, van Conny; Groen, Henk; Echten-Arends, Jannie van; Land, Jolande

doi:10.1093/humrep/deq258

Cited by 25 publications

(17 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This parameter in RT translocation carriers was lower than in the other groups. At the same time, most aberrations are found in azoospermic and severely oligozoospermic men [9] what is partially in agreement with the results of our study. However, other authors did observe such high correlation in subgroups of infertile men [26,27].…”

Section: Sperm Motility (%)supporting

confidence: 93%

“…Somatic chromosomal rearrangements are found in infertile men approximately 10 times more often than in the general population [7]. The prevalence of balanced autosomal translocations in the population of infertile men has been reported to be in the range of 1.6-6.6% [8][9][10]. Reciprocal translocations (RCT) are the replacement of genetic material between two or more chromosomes that does not result in the loss of genetic material [5].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sperm parameters and DNA fragmentation of balanced chromosomal rearrangements carriers

Pastuszek¹,

Kiewisz²,

Kulwikowska³

et al. 2016

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

Introduction. Somatic chromosomal rearrangements that occur in infertile males are thought to be one of the major genetic factors influencing male infertility. The objective of this retrospective study was to evaluate sperm parameters in a group of patients with balanced translocations. Material and methods. We analyzed semen of 84 balanced somatic translocation carriers [35 Robertsonian translocation (RT group) and 49 reciprocal translocation (RCT group)] and 57 men with normal karyotype (control group). Semen samples were evaluated for sperm concentration, its motion parameters and vitality, round cell number (CASA) and DNA fragmentation index (TUNEL). Cytogenetic evaluation was also performed for each study participant. Results. Sperm concentrations were lower when comparing the RT group to the control (p < 0.001) and RCT groups (p < 0.05). Occurrence of abnormal sperm concentration was more common among RT carriers (74.3%) than in the other groups (42.9% in RCT group and 28.1% in control group). The sperm progressive motility and vitality in RT carriers (21.53% and 62.17%) were lower than in control group (39.77% and 77.47%, p < 0.001, respectively) and RCT carriers (31.47% and 76.17%, p < 0.001, respectively). The RCT carriers and the control group did not differ in regard to sperm concentration, progressive sperm motility, motility grade D and sperm vitality. There were no significant differences in DNA fragmentation in carriers of both studied structural chromosomal rearrangements in comparison to subjects with normal karyotype. Conclusions. RT carriers had significantly lower semen parameters in comparison to not only the subjects with normal karyotypes but also the RCT carriers.

show abstract

Section: Sperm Motility (%)supporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Sperm parameters and DNA fragmentation of balanced chromosomal rearrangements carriers

Pastuszek¹,

Kiewisz²,

Kulwikowska³

et al. 2016

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

show abstract

“…In the studied group, 35 % of patients with azoospermia and 12.8 % of patients with oligozoospermia displayed chromosomal abnormalities. It should be mentioned, that most studies report approximately 15-17 % (from 11 % to 24 %) of patients with azoospermia and 2-16 % of patients with oligozoospermia to have chromosomal abnormalities [6,7,[18][19][20][21][22][23][24]. Such variability among different series is likely to be related to a dissimilar composition of the studied populations, mostly to the severity of male factor.…”

Section: Discussionmentioning

confidence: 99%

Chromosomal abnormalities in patients with oligozoospermia and non-obstructive azoospermia

Pylyp

Spinenko²,

Verhoglyad³

et al. 2013

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To assess the frequency and types of chromosomal abnormalities in 204 Ukrainian patients with non-obstructive azoospermia and oligozoospermia and 87 men with normozoospermia. Methods Cytogenetic studies were performed on peripheral blood lymphocyte samples of 164 men with oligozoospermia, 40 men with non-obstructive azoospermia and 87 men with normozoospermia attending infertility clinic. Results Chromosomal abnormalities were detected in 17 % of patients with sperm disorders: in 35 % of men with azoospermia and in 12.7 % of men with oligozoospermia. The frequency of chromosomal abnormalities in patients with sperm disorders was significantly higher, than in patients with normozoospermia (P=0.0001). An increase in the incidence of chromosomal abnormalities with the decrease of sperm count was observed. Chromosomal abnormalities were detected in 1.1 % of patients with normozoospermia, 6.5 % of patients with mild oligozoospermia (sperm count 5-15 ×10 6 /ml), 18.4 % of patients with severe oligozoospermia (sperm count <5 ×10 6 /ml) and 35 % of patients with azoospermia. A significant increase in the frequency of chromosomal abnormalities in patients with severe oligozoospermia was observed when compared to mild oligozoospermia (P=0.01). A statistically significant association (P= 0.02) of chromosomal abnormalities and sex chromosome abnormalities (P=0.0001) with azoospermia when compared to oligozoospermia was observed. Conclusions Our results highlight the importance of cytogenetic studies in patients with oligozoospermia (both mild and severe) and non-obstructive azoospermia. The presence of chromosomal abnormalities influences significantly the fertility treatment protocols, as well as provides a definite diagnosis to couples suffering from infertility.

show abstract

“…While most infertile men have a normal karyotype, it is known that the incidence of karyotypic chromosome abnormalities is higher in infertile males than in the general population. Azoospermic men have even a higher occurrence of karyotypic chromosomal abnormalities in comparison to men with severely diminished sperm parameters [Dul et al 2010]. Preimplantation genetic diagnosis (PGD) could be used to increase pregnancy rates in women at high risk of aneuploidy, such as patients with advanced maternal age, recurrent IVF failures, and pregnancy losses.…”

Section: Discussionmentioning

confidence: 99%

A comparison of ejaculated and testicular spermatozoa aneuploidy rates in patients with high sperm DNA damage

Moskovtsev¹,

Alladin²,

Lo³

et al. 2012

Systems Biology in Reproductive Medicine

View full text Add to dashboard Cite

Testicular spermatozoa are utilized to achieve pregnancy in couples with severe male factor infertility. Several studies suggest that aneuploidy rates in spermatozoa are elevated at the testicular level in infertile patients compared to ejaculates of normal controls. However, essential data regarding aneuploidy rates between ejaculated and testicular spermatozoa in the same individuals is lacking. The purpose of our study was to compare aneuploidy rates at the testicular and post-testicular level from the same patients with persistently high sperm DNA damage. Ejaculates and testicular biopsies were obtained from eight patients with persistently high DNA damage (>30%). Both ejaculated and testicular samples were analyzed for sperm DNA damage and sperm aneuploidy for chromosomes 13, 18, 21, X, and Y. In addition, semen samples from ten normozoospermic men presenting for fertility evaluation served as a control group. A strong correlation between the alteration of spermatogenesis and chromatin deterioration was observed in our study. In the same individuals, testicular samples showed a significantly lower DNA damage compared to ejaculated spermatozoa (14.9% ± 5.0 vs. 40.6% ± 14.8, P < 0.05), but significantly higher aneuploidy rates for the five analyzed chromosomes (12.41% ± 3.7 vs. 5.77% ± 1.2, P < 0.05). While testicular spermatozoa appear favourable for ICSI in terms of lower DNA damage, this potential advantage could be offset by the higher aneuploidy rates in testicular spermatozoa.

show abstract

Who should be screened for chromosomal abnormalities before ICSI treatment?

Cited by 25 publications

References 28 publications

Sperm parameters and DNA fragmentation of balanced chromosomal rearrangements carriers

Sperm parameters and DNA fragmentation of balanced chromosomal rearrangements carriers

Chromosomal abnormalities in patients with oligozoospermia and non-obstructive azoospermia

A comparison of ejaculated and testicular spermatozoa aneuploidy rates in patients with high sperm DNA damage

Contact Info

Product

Resources

About