Whitefly (Bemisia tabaci) genome project: analysis of sequenced clones from egg, instar, and adult (viruliferous and non-viruliferous) cDNA libraries

Leshkowitz, Dena; Gazit, S.; Reuveni, Eli; Ghanim, Murad; Czosnek, Henryk; McKenzie, Cindy L.; Shatters, Robert G.; Brown, Judith K.

doi:10.1186/1471-2164-7-79

Cited by 80 publications

(51 citation statements)

References 35 publications

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“…While there are reports of whitefly genes involved in insecticide resistance [83]; viral [84] and bacterial infections [85], few reports presented how entomopathogenic fungus regulates its gene expression during the infection of living insects. The few existing papers only examined the fungal response on specific in vitro tissues of insects rather than on live insects [24], [86], [87].…”

Section: Discussionmentioning

confidence: 99%

Analysis of Whitefly Transcriptional Responses to Beauveria bassiana Infection Reveals New Insights into Insect-Fungus Interactions

Xia¹,

Zhang²,

Zhang³

et al. 2013

PLoS ONE

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BackgroundThe fungal pathogen, Beauveria bassiana, is an efficient biocontrol agent against a variety of agricultural pests. A thorough understanding of the basic principles of insect-fungus interactions may enable the genetic modification of Beauveria bassiana to enhance its virulence. However, the molecular mechanism of insect response to Beauveria bassiana infection is poorly understood, let alone the identification of fungal virulent factors involved in pathogenesis.Methodology/Principal FindingsHere, next generation sequencing technology was applied to examine the expression of whitefly (Bemisia tabaci) genes in response to the infection of Beauveria bassiana. Results showed that, compared to control, 654 and 1,681genes were differentially expressed at 48 hours and 72 hours post-infected whiteflies, respectively. Functional and enrichment analyses indicated that the DNA damage stimulus response and drug metabolism were important anti-fungi strategies of the whitefly. Mitogen-activated protein kinase (MAPK) pathway was also likely involved in the whitefly defense responses. Furthermore, the notable suppression of general metabolism and ion transport genes observed in 72 hours post-infected B. tabaci might be manipulated by fungal secreted effectors. By mapping the sequencing tags to B. bassiana genome, we also identified a number of differentially expressed fungal genes between the early and late infection stages. These genes are generally associated with fungal cell wall synthesis and energy metabolism. The expression of fungal cell wall protein genes might play an important role in fungal pathogenesis and the dramatically up-regulated enzymes of carbon metabolism indicate the increasing usage of energy during the fungal infection.Conclusions/SignificanceTo our knowledge, this is the first report on the molecular mechanism of fungus-whitefly interactions. Our results provide a road map for future investigations on insect-pathogen interactions and genetically modifying the fungus to enhance its efficiency in whitefly control.

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Section: Discussionmentioning

confidence: 99%

Analysis of Whitefly Transcriptional Responses to Beauveria bassiana Infection Reveals New Insights into Insect-Fungus Interactions

Xia¹,

Zhang²,

Zhang³

et al. 2013

PLoS ONE

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“…The annotation of differentially expressed gene sequences was confirmed using the published MEAM1 and MED whitefly genomes (Xie et al 2017;Chen et al 2016), as well as thousands of expressed sequence tags from Be. tabaci (Leshkowitz et al, 2006;Wang et al, 2011). We used a cut-off E-value of 10 −5 .…”

Section: General Overview Of the Transcriptome Response Tomentioning

confidence: 99%

Levels of the endosymbiont Rickettsia in the whitefly Bemisia tabaci are influenced by the expression of vitellogenin

Brumin

Lebedev

Kontsedalov

et al. 2019

Insect Molecular Biology

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Bacterial endosymbionts play essential roles in the biology of their arthropod hosts by interacting with internal factors in the host. The whitefly Bemisia tabaci is a worldwide agricultural pest and a supervector for more than 100 plant viruses. Like many other arthropods, Be. tabaci harbours a primary endosymbiont, Porteira aleyrodidarum, and an array of secondary endosymbionts that coexist with Portiera inside bacteriocyte cells. Unlike all of the other secondary symbionts that infect Be. tabaci, Rickettsia has been shown to be an exception by infecting insect organs and not colocalizing with Portiera, and has been shown to significantly impact the insect biology and its interactions with the environment. Little is known about the molecular interactions that underlie insect–symbiont interactions in general, and particularly Be. tabaci–Rickettsia interactions. Here we performed transcriptomic analysis and identified vitellogenin as an important protein that influences the levels of Rickettsia in Be. tabaci. Vitellogenin expression levels were lower in whole insects, but higher in midguts of Rickettsia‐infected insects. Immunocapture‐PCR assay showed interaction between vitellogenin and Rickettsia, whereas silencing of vitellogenin resulted in nearly complete disappearance of Rickettsia from midguts. Altogether, these results suggest that vitellogenin plays an important role in influencing the levels of Rickettsia in Be. tabaci.

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“…The availability of large cDNA and genomic DNA sequence data sets for M. persicae has paved the way for functional genomics studies, which have the potential to identify aphid genes involved in resistance to plant defenses and insecticides (Leshkowitz et al, 2006; Ramsey et al, 2007). The goal of the current study is to examine the nicotine resistance, growth, and transcriptional responses of tobacco-adapted and non-adapted M. persicae lineages.…”

Section: Introductionmentioning

confidence: 99%

Adaptation to Nicotine Feeding in Myzus persicae

et al. 2014

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Lineages of the generalist hemipteran herbivore Myzus persicae (green peach aphid) that have expanded their host range to include tobacco often have elevated nicotine tolerance. The tobacco-adapted M. persicae lineage used in this study was able to reproduce on nicotine-containing artificial diets at concentrations that were 15-fold higher than those that were lethal to a non-adapted M. persicae lineage. Fecundity of the nicotine-tolerant M. persicae lineage was increased by 100 μM nicotine in artificial diet, suggesting that this otherwise toxic alkaloid can serve as a feeding stimulant at low concentrations. This lineage also was pre-adapted to growth on tobacco, exhibiting no drop in fecundity when it was moved onto tobacco from a different host plant. Although growth of the non-tobacco-adapted M. persicae lineage improved after three generations on tobacco, this higher reproductive rate was not associated with increased nicotine tolerance. M. persicae gene expression microarrays were used to identify transcripts that are up-regulated in response to nicotine in the tobacco-adapted lineage. Induced expression was found for CYP6CY3, which detoxifies nicotine in M. persicae, other genes encoding known classes of detoxifying enzymes, as well as genes encoding secreted M. persicae salivary proteins.

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Whitefly (Bemisia tabaci) genome project: analysis of sequenced clones from egg, instar, and adult (viruliferous and non-viruliferous) cDNA libraries

Cited by 80 publications

References 35 publications

Analysis of Whitefly Transcriptional Responses to Beauveria bassiana Infection Reveals New Insights into Insect-Fungus Interactions

Analysis of Whitefly Transcriptional Responses to Beauveria bassiana Infection Reveals New Insights into Insect-Fungus Interactions

Levels of the endosymbiont Rickettsia in the whitefly Bemisia tabaci are influenced by the expression of vitellogenin

Adaptation to Nicotine Feeding in Myzus persicae

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