‘White wave’ analysis of epithelial scratch wound healing reveals how cells mobilise back from the leading edge in a myosin-II-dependent fashion

Matsubayashi, Yutaka; Razzell, William; Martin, Paul

doi:10.1242/dev.066944

Cited by 4 publications

(6 citation statements)

References 1 publication

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“…In monolayer epithelial sheets, a finite number of cells are mobilized to close a scratch, and the mobilization of distant cells is independent of the advancement of the leading cells (Matsubayashi et al, 2011). However, in the Drosophila embryo wound model, the cells away from the wound edge stretch toward the wound and show actomyosin-dependent shrinkage of cell-cell junctions with their neighbors to drive cell intercalation events and thus close the wound (Razzell et al, 2014).…”

Section: Epithelializationmentioning

confidence: 99%

Palatogenesis and cutaneous repair: A two‐headed coin

Biggs

Goudy

Dunnwald

2014

Developmental Dynamics

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The reparative mechanism that operates following post-natal cutaneous injury is a fundamental survival function that requires a well-orchestrated series of molecular and cellular events. At the end, the body will have closed the hole using processes like cellular proliferation, migration, differentiation and fusion. These processes are similar to those occurring during embryogenesis and tissue morphogenesis. Palatogenesis, the formation of the palate from two independent palatal shelves growing towards each other and fusing, intuitively, shares many similarities with the closure of a cutaneous wound from the two migrating epithelial fronts. In this review, we summarize the current information on cutaneous development, wound healing, palatogenesis and orofacial clefting and propose that orofacial clefting and wound healing are conserved processes that share common pathways and gene regulatory networks.

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Section: Epithelializationmentioning

confidence: 99%

Palatogenesis and cutaneous repair: A two‐headed coin

Biggs

Goudy

Dunnwald

2014

Developmental Dynamics

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“…Therefore, TC migration does not rely on actomyosin contractibility, posing the question which molecular players might be involved in TC force generation. Interestingly, collective movement of cells depends on actin-based filopodia and lamellipodia (Mayor and Etienne-Manneville, 2016) and was shown in several cases to be independent of MyoII activity (Matsubayashi et al, 2011; Serra-Picamal et al, 2012). Furthermore, several studies showed that a down-regulation of MyoII is required for effective collective cell migration (Hidalgo-Carcedo et al, 2011; Omelchenko and Hall, 2012; Yamada and Nelson, 2007).…”

Section: Discussionmentioning

confidence: 99%

Myosin II activity is not required forDrosophilatracheal branching morphogenesis

Ochoa-Espinosa

Harmansa

Caussinus

et al. 2017

Preprint

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“…If |d| > 0, cells captured in those pixels moved and the pixels appear white in the resulting new image, but if cells did not move and |d| = 0, then the respective pixel remains black in the resulting image. It could be shown that this visualization of cell migration leads to a "white wave" spreading [33]. A unique feature of this tool is the visualization of cell migration over time.…”

Section: Tools and Algorithms For Data Analysismentioning

confidence: 99%

“…White Wave Model with ImageJ Difference in each pixel of two consecutive micrographs is visualized in a binary picture [33] Shows cell migration over time in form of "White Wave" Cannot distinguish direction of single cell migration;…”

Section: Consmentioning

confidence: 99%

In vitro wound healing assays – state of the art

et al. 2016

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Wound healing is essential for the restoration of the barrier function of the skin. During this process, cells at the wound edges proliferate and migrate, leading to re-epithelialization of the wound surface. Wound healing assays are used to study the molecular mechanisms of wound repair, as well as in the investigation of potential therapeutics and treatments for improved healing. Numerous models of wound healing have been developed in recent years. In this review, we focus on in vitro assays, as they allow a fast, cost-efficient and ethical alternative to animal models. This paper gives a general overview of 2-dimensional (2D) cell monolayer assays by providing a description of injury methods, as well as an evaluation of each assay's strengths and limitations. We include a section reviewing assays performed in 3-dimensional (3D) culture, which employ bioengineered skin models to capture complex wound healing mechanics like cell-matrix interactions and the interplay of different cell types in the healing process. Finally, we discuss in detail available software tools and algorithms for data analysis.

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‘White wave’ analysis of epithelial scratch wound healing reveals how cells mobilise back from the leading edge in a myosin-II-dependent fashion

Cited by 4 publications

References 1 publication

Palatogenesis and cutaneous repair: A two‐headed coin

Palatogenesis and cutaneous repair: A two‐headed coin

Myosin II activity is not required forDrosophilatracheal branching morphogenesis

In vitro wound healing assays – state of the art

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