WHITE STRIPE LEAF8, encoding a deoxyribonucleoside kinase, is involved in chloroplast development in rice

Liu, L. L.; You, Jichun; Zhu, Ziming; Chen, K. Y.; Hu, Min; Gu, H.; Liu, Z. W.; Wang, Z. Y.; Wang, Y. H.; Liu, S. J.; Chen, L. M.; Liu, Xingye; Tian, Yunlu; Zhou, Shirong; Jiang, Ling; Wan, Jianmin

doi:10.1007/s00299-019-02470-6

Cited by 11 publications

(13 citation statements)

References 55 publications

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“…Wild-type and spl42 mutant plants were grown in Nanjing (Jiangsu province) and Lingshui (Hainan province) under natural conditions. For temperature-sensitive treatments, plants in the growth chamber (GXM-258B, Ningbo) were treated with a 16 h light/8 h darkness photoperiod at a constant temperature of 20 °C, 25 °C, or 30 °C, respectively [ 44 ]. The thousand-grain weight, seed length, and width were examined by a seed phenotyping system (SC-G automatic test seed analysis software, Hangzhou).…”

Section: Methodsmentioning

confidence: 99%

Isolation and Characterization of SPOTTED LEAF42 Encoding a Porphobilinogen Deaminase in Rice

Liu

Wang²,

Tian³

et al. 2023

Plants

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The formation and development of chloroplasts play a vital role in the breeding of high-yield rice (Oryza sativa L.). Porphobilinogen deaminases (PBGDs) act in the early stage of chlorophyll and heme biosynthesis. However, the role of PBGDs in chloroplast development and chlorophyll production remains elusive in rice. Here, we identified the spotted leaf 42 (spl42) mutant, which exhibited a reddish-brown spotted leaf phenotype. The mutant showed a significantly lower chlorophyll content, abnormal thylakoid morphology, and elevated activities of reactive oxygen species (ROS)-scavenging enzymes. Consistently, multiple genes related to chloroplast development and chlorophyll biosynthesis were significantly down-regulated, whereas many genes involved in leaf senescence, ROS production, and defense responses were upregulated in the spl42 mutant. Map-based cloning revealed that SPL42 encodes a PBGD. A C-to-T base substitution occurred in spl42, resulting in an amino acid change and significantly reduced PBGD enzyme activity. SPL42 targets to the chloroplast and interacts with the multiple organelle RNA editing factors (MORFs) OsMORF8-1 and OsMORF8-2 to affect RNA editing. The identification and characterization of spl42 helps in elucidating the molecular mechanisms associated with chlorophyll synthesis and RNA editing in rice.

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Section: Methodsmentioning

confidence: 99%

Isolation and Characterization of SPOTTED LEAF42 Encoding a Porphobilinogen Deaminase in Rice

Liu

Wang²,

Tian³

et al. 2023

Plants

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“…The activity of the endogenous antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) was determined using commercial ELISA kits (Mmbio, Jiangsu, China). Sample preparation and test procedures were carried out in strict accordance with the manufacturer’s instructors [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

“…Dehydrated samples were embedded in epoxy resin, thinly sliced with an EM UC7 ultramicrotome (Leica, Wetzlar, Germany). Chloroplast ultramicrostructure was observed and photographed using an HT-7700 transmission electron microscope (TEM) (Hitachi, Ibaraki, Japan) [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

Integrated Physiological, Transcriptomic, and Proteomic Analyses Reveal the Regulatory Role of Melatonin in Tomato Plants’ Response to Low Night Temperature

et al. 2022

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Melatonin is a direct free radical scavenger that has been demonstrated to increase plants’ resistance to a variety of stressors. Here, we sought to examine the effect of melatonin on tomato seedlings subjected to low night temperatures using an integrated physiological, transcriptomic, and proteomic approach. We found that a pretreatment with 100 μM melatonin increased photosynthetic and transpiration rates, stomatal apertures, and peroxidase activity, and reduced chloroplast damage of the tomato plant under a low night temperature. The melatonin pretreatment reduced the photoinhibition of photosystem I by regulating the balance of both donor- and acceptor-side restriction of PSI and by increasing electron transport. Furthermore, the melatonin pretreatment improved the photosynthetic performance of proton gradient regulation 5 (SlPGR5) and SlPGR5-like photosynthetic phenotype 1 (SlPGRL1)-suppressed transformants under a low night temperature stress. Transcriptomic and proteomic analyses found that the melatonin pretreatment resulted in the upregulation of genes and proteins related to transcription factors, signal transduction, environmental adaptation, and chloroplast integrity maintenance in low night temperature-stressed tomato plants. Collectively, our results suggest that melatonin can effectively improve the photosynthetic efficiency of tomato plants under a low night temperature and provide novel insights into the molecular mechanism of melatonin-mediated abiotic stress resistance.

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“…The mechanism of leaf color variation mainly includes a blocked chlorophyll metabolic pathway (Lee et al 2005; Yang et al 2016) and impaired chloroplast development (Kusumi et al 2004; Sugimoto et al 2004). Among them, many rice white-striped leaf mutants have been identi ed, and their regulation by molecular mechanisms has been deeply studied (Aluru and Rodermel 2004), including the study of ST1 (Yoo et al 2009), WSL (Tan et al 2014), WSL3 (Wang et al 2016), WFSL1 (Ge et al 2017), WSL4 (Wang et al 2017), WSL5 (Liu et al 2018), WSL6 (Sun et al 2019), WSL8 (Liu et al 2020), WSL9 (Zhu et al 2020), WSL12 (Ye et al 2016), WSP1 (Zhang et al 2017), YSS1 (Zhou et al 2017) and GRA78 (Wang et al 2019) genes. ST1 encodes a small subunit RNRS1 of ribonucleic acid reductase RNR, which is involved in the replication of chloroplast DNA during chlorophyll synthesis (Yoo et al 2009).…”

Section: Introductionmentioning

confidence: 99%

“…WSL5 encodes a chloroplast-targeted pentapeptide repeat protein involved in chloroplast gene RNA splicing (Liu et al 2018); WSL6 encodes an era-type guanosine-5'-triphosphate (GTP)-binding protein (Sun et al 2019). WSL8 encodes a deoxyribonucleoside kinase involved in rice chloroplast development (Liu et al 2020). WSL9 encodes a novel protein with an HNH domain (Zhu et al 2020); WGL2 encodes a conserved ribosomal protein that is an important component of rice chloroplast development (Ye et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Characterization and Fine Mapping of a White Stripe Leaf Mutant in Rice

Xiang

et al. 2022

Preprint

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Leaf color affects the efficiency of photosynthesis, and leaf color mutants are important genetic materials for studying the mechanisms of photosynthesis, chlorophyll biosynthesis, and chloroplast development in rice. In this study, a white-striped leaf mutant, wst1, was obtained from the mutant population of the indica restorer line ‘Chuanhui 907’ (R907) when treated by 60Co-γ radiation. Compared to the wild-type, the wst1 mutant showed normal leaf color before tillering and white stripes on the leaf and leaf sheaths after tillering. The chlorophyll and carotenoid contents were significantly reduced, and the thylakoids of chloroplasts developed abnormalities in wst1 plants in the tillering stage. The results of agronomic trait analysis showed that the number of effective panicles, plant height, seed setting rate, and 1000-grain weight of the wst1 mutant were significantly lower than those of the wild-type. Genetic analysis revealed that the phenotype of the wst1 mutant is controlled by a pair of recessive nuclear genes. The candidate gene was mapped to a 72 kb region between the InDel markers M6 and M12 on the short arm of chromosome 1 using molecular marker linkage analysis. Candidate genes were sequenced on the interval, and a G base was replaced by A at the 6972nd position on the 16th exon of LOC_Os01g01920, which encoded a previously reported protein containing the HD domain, WSF3/WFSL1, leading to alternative splicing, causing a 104 bp deletion in the coding region, and resulting in mistranslation after the 490 amino acid of the encoded protein translation in wst1. RT-qPCR analysis showed that the expression levels of most genes related to chlorophyll synthesis and chloroplast development were significantly altered in wst1 plants. Our study identified a novel allele of wsf3 and wfsl1 mutant and provided a new genetic resource and theoretical basis for further understanding of the molecular mechanism of WST1 gene regulation of white-striped leaves in rice.

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WHITE STRIPE LEAF8, encoding a deoxyribonucleoside kinase, is involved in chloroplast development in rice

Cited by 11 publications

References 55 publications

Isolation and Characterization of SPOTTED LEAF42 Encoding a Porphobilinogen Deaminase in Rice

Isolation and Characterization of SPOTTED LEAF42 Encoding a Porphobilinogen Deaminase in Rice

Integrated Physiological, Transcriptomic, and Proteomic Analyses Reveal the Regulatory Role of Melatonin in Tomato Plants’ Response to Low Night Temperature

Characterization and Fine Mapping of a White Stripe Leaf Mutant in Rice

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