WHITE STRIPE LEAF4 Encodes a Novel P-Type PPR Protein Required for Chloroplast Biogenesis during Early Leaf Development

Wang, Ying; Ren, Yulong; Zhou, Kunneng; Liu, Linglong; Wang, Jiulin; Xu, Yang; Zhang, Huan; Zhang, Long; Feng, Zhiming; Wang, Liwei; Ma, Weiwei; Wang, Yunlong; Guo, Xiuping; Zhang, Xin; Lei, Cailin; Cheng, Zhijun

doi:10.3389/fpls.2017.01116

Cited by 62 publications

(63 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Total RNA was isolated from grains at 9 DAF using Trans Zol Up (TransGen Co., Beijing, China), followed by hybridization as previously described (Wang Y. et al ., 2017). Probes for Northern blotting, were labeled using a DIG Northern Starter Kit (Roche) according to the instruction manual.…”

Section: Methodsmentioning

confidence: 99%

Rice FLOURY ENDOSPERM10 encodes a pentatricopeptide repeat protein that is essential for the trans‐splicing of mitochondrial nad1 intron 1 and endosperm development

Ren

Cai

et al. 2019

New Phytologist

Self Cite

View full text Add to dashboard Cite

Summary Endosperm, the major storage organ in cereal grains, determines grain yield and quality. Despite the fact that a role for P‐type pentatricopeptide repeat (PPR) proteins in the regulation of endosperm development has emerged, molecular functions of many P‐type PPR proteins remain obscure. Here, we report a rice endosperm defective mutant, floury endosperm10 (flo10), which developed smaller starch grains in starchy endosperm and abnormal cells in the aleurone layer. Map‐based cloning and rescued experiments showed that FLO10 encodes a P‐type PPR protein with 26 PPR motifs, which is localized to mitochondria. Loss of function of FLO10 affected the trans‐splicing of the mitochondrial nad1 intron 1, which was accompanied by the increased accumulation of the nad1 exon 1 and exons 2–5 precursors. The failed formation of mature nad1 led to a dramatically decreased assembly and activity of complex I, reduced ATP production, and changed mitochondrial morphology. In addition, loss of function of FLO10 significantly induced an alternative respiratory pathway involving alternative oxidase. These results reveal that FLO10 plays an important role in the maintenance of mitochondrial function and endosperm development through its effect on the trans‐splicing of the mitochondrial nad1 intron 1 in rice.

show abstract

Section: Methodsmentioning

confidence: 99%

Rice FLOURY ENDOSPERM10 encodes a pentatricopeptide repeat protein that is essential for the trans‐splicing of mitochondrial nad1 intron 1 and endosperm development

Ren

Cai

et al. 2019

New Phytologist

Self Cite

View full text Add to dashboard Cite

show abstract

“…The rpoB editing defect in Dular was potentially a secondary effect of the chloroplast ribosome defect. Wang et al (2017) reported that in a rice white stripe leaf4 (wsl4) mutant, WSL4 encoded a novel P-family PPR protein, and the wsl4 mutant caused defects in the splicing of atpF, ndhA, rpl2, and rps12 and in the editing efficiency of rpoB at C545 and C560. The rpoB editing defects at C545 and C560 in wsl4 may be indirectly responsible for the efficient rpoB editing.…”

Section: New Phytologistmentioning

confidence: 99%

“…However, little or no functional redundancy among PPR proteins has been found in higher plants, this situation indicates that the functions of PPR proteins are highly diversified in these plants. Accumulating evidence has revealed that PPR proteins are involved in a wide range of biological processes, such as cytoplasmic male sterility (CMS; Gaborieau et al ., ; Igarashi et al ., ; Liu et al ., , ), embryogenesis (Cai et al ., ; Li et al ., ; Tadini et al ., ), retrograde signaling (Sun et al ., ), and low temperature stress (Wu et al ., ; Wang et al ., ). However, the molecular mechanisms underlying the roles of PPR proteins in low temperature stress remain largely unknown.…”

Section: Introductionmentioning

confidence: 97%

“…Accumulating evidence has revealed that PPR proteins are involved in a wide range of biological processes, such as cytoplasmic male sterility (CMS; Gaborieau et al, *These authors contributed equally to this work. 2016; Igarashi et al, 2016;Liu et al, 2016Liu et al, , 2017, embryogenesis Li et al, 2018;Tadini et al, 2018), retrograde signaling (Sun et al, 2016), and low temperature stress (Wu et al, 2016b;Wang et al, 2017). However, the molecular mechanisms underlying the roles of PPR proteins in low temperature stress remain largely unknown.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The RNA editing factor DUA1 is crucial to chloroplast development at low temperature in rice

Cui

Wang

et al. 2018

New Phytologist

View full text Add to dashboard Cite

Summary Low temperature stress hinders plant growth and chloroplast development and can limit the geographic range of cultivars. In rice, japonica cultivars have greater chilling tolerance than indica cultivars, but the molecular mechanism underlying chilling tolerance is unclear. Here, we report an RNA‐binding protein, DUA1, cloned from the indica cultivar Dular, which exhibits a deficiency in chloroplast development at an early stage of development under low‐temperature conditions. DUA1 shares high sequence homology with the pentatricopeptide repeat family and functions in plastid RNA editing under low‐temperature conditions. Our data suggest that DUA1 can bind to the plastid‐encoded rps8‐182 transcript and disruption of DUA1 activity impairs editing. The RNA editing cofactor WSP1, a partner of DUA1, also participates in chloroplast development at low temperature. Western blot analysis indicates that WSP1 enhances DUA1 stability under low temperatures. DUA1 sequence analyses of rice core germplasm revealed that three major haplotypes of DUA1 and one haplotype showed substantial differences in chlorophyll content under low‐temperature conditions. Variation at DUA1 may play an important role in the adaptation of rice to different growing regions.

show abstract

“…2014). The P-family PPR mutant wsl4 , which exhibits white-striped leaves before the 5-leaf stage, has defective chloroplast RNA group II intron splicing (Wang et al . 2017).…”

Section: Introductionmentioning

confidence: 99%

WSL5, a pentatricopeptide repeat protein, is essential for chloroplast biogenesis in rice under cold stress

Liu

Lan

Huang

et al. 2017

Preprint

View full text Add to dashboard Cite

Abstact 23Chloroplasts play an essential role in plant growth and development, and cold has a great effect 24 on chloroplast development. Although many genes or regulators involved in chloroplast 25 biogenesis and development have been isolated and characterized, identification of novel 26 components associated with cold is still lacking. In this study, we reported the functional 27 characterization of white stripe leaf 5 (wsl5) mutant in rice. The mutant developed white-striped 28 leaves during early leaf development and was albinic when planted under cold stress. Genetic and 29 molecular analysis revealed that WSL5 encodes a novel chloroplast-targeted pentatricopeptide 30 repeat protein. RNA-seq analysis showed that expression of nuclear-encoded photosynthetic 31 genes in the mutant was significantly repressed, and expression of many chloroplast-encoded 32 genes was also significantly changed. Notably, the WSL5 mutation caused defects in editing of 33 rpl2 and atpA, and in splicing of rpl2 and rps12. Chloroplast ribosome biogenesis was impaired 34 under cold stress. We propose that WSL5 is required for normal chloroplast development in rice 35 under cold stress. 36 37Key words: chloroplast biogenesis, cold stress, Oryza sativa, RNA-seq, RNA splicing, WSL5. 38 39 All rights reserved. No reuse allowed without permission.(which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint . http://dx.doi.org/10.1101/239905 doi: bioRxiv preprint first posted online Dec. 26, 2017; Introduction 40Cold is an important environmental factor affecting chloroplast development and growth in juvenile 41 plants and sudden low-temperature periods that often occur during early seedling development in 42 spring can directly affect production (Kusumi and Iba, 2014). Rice seedlings are susceptible to cold 43 stress with an impact that ultimately affects grain yield (Liu et al. 2013). Therefore, cold stress is a 44 common problem that affects grain production, and rice varieties with increased cold tolerance are 45 preferred (Zhao et al. 2017). Many studies have suggested that plants can regulate early chloroplast 46 development under cold stress. In certain virescent mutants the chlorophyll content in young leaves is 47 low, but gradually increases to normal levels as they develop (Yoo et al. 2009). Temperature-sensitive 48 virescent mutants were used to study mechanisms regulating chloroplast development in seedlings 49 under cold stress conditions, and many genes were identified, such as V3, St1, OsV4, TCD9 and TSV 50 (Yoo et al. 2009; Gong et al. 2014; Jiang et al. 2014; Sun et al. 2017). However, the mechanisms of 51 chloroplast development in rice seedlings under cold stress remain poorly understood. 52Chloroplasts are essential for photosynthesis and have crucial roles in plant development and growth 53 by fixation of CO 2 and biosynthesis of carbon skeletons as well as other physiological processes (Jarvis 54and López-Juez...

show abstract

WHITE STRIPE LEAF4 Encodes a Novel P-Type PPR Protein Required for Chloroplast Biogenesis during Early Leaf Development

Cited by 62 publications

References 87 publications

Rice FLOURY ENDOSPERM10 encodes a pentatricopeptide repeat protein that is essential for the trans‐splicing of mitochondrial nad1 intron 1 and endosperm development

Rice FLOURY ENDOSPERM10 encodes a pentatricopeptide repeat protein that is essential for the trans‐splicing of mitochondrial nad1 intron 1 and endosperm development

The RNA editing factor DUA1 is crucial to chloroplast development at low temperature in rice

WSL5, a pentatricopeptide repeat protein, is essential for chloroplast biogenesis in rice under cold stress

Contact Info

Product

Resources

About