White spot syndrome virus (WSSV) infection alters gut histopathology and microbiota composition in crayfish (Procambarus clarkii)

Xue, Mingyang; Jiang, Nan; Fan, Yuding; Yang, Tao; Li, Mao; Liu, Wenzhi; Li, Yiqun; Li, Bo; Zeng, Lingbing; Zhou, Yong

doi:10.1016/j.aqrep.2022.101006

Cited by 11 publications

(9 citation statements)

References 33 publications

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“…The results of histological analysis showed that severe intestinal mucosal damage was observed in the intestine of DIV1-infected shrimp, and the intestinal epithelial cells were completely detached from the basement membrane. Similarly, Xue et al found that WSSV-infected crayfish Procambarus clarkii exhibited worse intestinal histomorphology, with thinner intestinal walls and shorter intestinal villi compared to healthy crayfish (Xue et al, 2022). Liang et al found that the intestinal epithelial cells of L. vannamei infected with Vibrio parahaemolyticus were completely detached from the basement membrane (Liang et al, 2020).…”

Section: Discussionmentioning

confidence: 97%

“…found that WSSV-infected crayfish Procambarus clarkii exhibited worse intestinal histomorphology, with thinner intestinal walls and shorter intestinal villi compared to healthy crayfish (Xue et al, 2022). Liang et al found that the intestinal epithelial cells of L. vannamei infected with Vibrio parahaemolyticus were completely detached from the basement membrane (Liang et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

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Integrated analysis of intestinal microbiota and metabolomic reveals that decapod iridescent virus 1 (DIV1) infection induces secondary bacterial infection and metabolic reprogramming in Marsupenaeus japonicus

Zhong

Liao

et al. 2022

Preprint

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In recent years, with global warming and increasing marine pollution, some novel marine viruses have become widespread in the aquaculture industry, causing huge losses to the aquaculture industry. Decapod iridescent virus 1 (DIV1) is one of the newly discovered marine viruses that has been reported to be detected in a variety of farmed crustacean and wild populations. Previous studies have found that DIV1 can induce the Warburg effect. To further explore the effect of DIV1-induced metabolic reprogramming on Marsupenaeus japonicus intestinal metabolome and microbiota and the consequence on immune response, histological analysis, enzyme activity analysis and the integrated analysis of intestinal microbiome and metabolomics were performed in this study. The results showed that obvious injury in the intestinal mucosa was observed after DIV1 infection. The oxidative and antioxidant capacity of the shrimp intestine was unbalanced, the activity of lysozyme was decreased, and the activities of digestive enzymes were disordered, causing secondary bacterial infection. In addition, the increased abundance of harmful bacteria, such as Photobacterium and Vibrio, synergized with DIV1 to promote the Warburg effect and induce metabolic reprogramming, thereby providing material and energy for DIV1 replication. This study is the first to report the changes of intestinal microbiota and metabolites of M. japonicus under DIV1 infection, demonstrating that DIV1 can induce secondary bacterial infection and metabolic reprogramming, and several highly related bacteria and metabolites were screened as biomarkers. These biomarkers can be leveraged for diagnosis of pathogenic infections or incorporated as exogenous metabolites to enhance immune response.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Integrated analysis of intestinal microbiota and metabolomic reveals that decapod iridescent virus 1 (DIV1) infection induces secondary bacterial infection and metabolic reprogramming in Marsupenaeus japonicus

Zhong

Liao

et al. 2022

Preprint

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show abstract

“…The results of histological analysis showed that severe intestinal mucosal damage was observed in the intestine of DIV1-infected shrimp, some intestinal epithelial cells were detached from the basement membrane and the intestinal villus height was significantly reduced. Similarly, Xue et al found that WSSV-infected crayfish Procambarus clarkii exhibited worse intestinal histomorphology, with thinner intestinal walls and shorter intestinal villi compared to healthy crayfish (32). Liang et al found that the intestinal epithelial cells of L. vannamei infected with Vibrio parahaemolyticus were completely detached from the basement membrane (33).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, both the LEfSe cladogram and LDA score of LEfSe-PICRUSt showed that Photobacterium and Vibrio dominated in the DIV1infected group. Several previous studies had shown that WSSV infection can cause an increase in the relative abundance of opportunistic pathogens in the intestine of shrimp, such as Aeromonas, Arcobacter, Vibrio and Trichococcus (19,21,32). Photobacterium had been shown to cause various diseases in fish and shrimp (57-61) causing huge losses in aquaculture.…”

Section: Discussionmentioning

confidence: 99%

Integrated analysis of intestinal microbiota and metabolomic reveals that decapod iridescent virus 1 (DIV1) infection induces secondary bacterial infection and metabolic reprogramming in Marsupenaeus japonicus

Zhong

Liao

et al. 2022

Front. Immunol.

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In recent years, with global warming and increasing marine pollution, some novel marine viruses have become widespread in the aquaculture industry, causing huge losses to the aquaculture industry. Decapod iridescent virus 1 (DIV1) is one of the newly discovered marine viruses that has been reported to be detected in a variety of farmed crustacean and wild populations. Several previous studies have found that DIV1 can induce Warburg effect-related gene expression. In this study, the effects of DIV1 infection on intestinal health of shrimp were further explored from the aspects of histological, enzymatic activities, microorganisms and metabolites using Marsupenaeus japonicus as the object of study. The results showed that obvious injury in the intestinal mucosa was observed after DIV1 infection, the oxidative and antioxidant capacity of the shrimp intestine was unbalanced, the activity of lysozyme was decreased, and the activities of digestive enzymes were disordered, and secondary bacterial infection was caused. Furthermore, the increased abundance of harmful bacteria, such as Photobacterium and Vibrio, may synergized with DIV1 to promote the Warburg effect and induce metabolic reprogramming, thereby providing material and energy for DIV1 replication. This study is the first to report the changes of intestinal microbiota and Frontiers in Immunology frontiersin.org 01

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“…Microbiome function was predicted by using taxa abundance differentially expressed and the PICRUSt plug-in which mapped Greengenes IDs to the corresponding KEGG pathway. This tool has been used to predict the bacterial functional composition in crustaceans’ guts 29 . The predicted microbiome function was plotted by circular graphics using a log10 LDA score.…”

Section: Methodsmentioning

confidence: 99%

The emerging pathogen Enterocytozoon hepatopenaei drives a degenerative cyclic pattern in the hepatopancreas microbiome of the shrimp (Penaeus vannamei)

López‐Carvallo

Cruz‐Flores

Dhar

2022

Sci Rep

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The microsporidian Enterocytozoon hepatopenaei (EHP) is an emerging pathogen that causes substantial economic losses in shrimp (Penaeus spp.) aquaculture worldwide. To prevent diseases in shrimp, the manipulation of the gut microbiota has been suggested. However, prior knowledge of the host-microbiome is necessary. We assessed the modulation of the microbiome (bacteria/fungi) and its predicted functions over the course of disease progression in shrimp experimentally challenged with EHP for 30 days using high throughput 16S rRNA and ITS amplicon sequencing. Infection grade was assessed for the first time by quantitative digital histopathology. According to the infection intensity, three disease-stages (early/developmental/late) were registered. During the early-stage, EHP was not consistently detected, and a high diversity of potentially beneficial microorganisms related to nutrient assimilation were found. In the development-stage, most of the shrimp start to register a high infection intensity related to a decrease in beneficial microorganisms and an increase in opportunistic/pathogenic fungi. During late-stage, animals displayed different infection intensities, showed a displacement of beneficial microorganisms by opportunistic/pathogenic bacteria and fungi related to pathogen infection processes and depletion of energetic reserves. The degenerative cyclic pattern of EHP infection and its effects on beneficial microorganisms and beneficial functions of the shrimp hepatopancreas microbiome are discussed.

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White spot syndrome virus (WSSV) infection alters gut histopathology and microbiota composition in crayfish (Procambarus clarkii)

Cited by 11 publications

References 33 publications

Integrated analysis of intestinal microbiota and metabolomic reveals that decapod iridescent virus 1 (DIV1) infection induces secondary bacterial infection and metabolic reprogramming in Marsupenaeus japonicus

Integrated analysis of intestinal microbiota and metabolomic reveals that decapod iridescent virus 1 (DIV1) infection induces secondary bacterial infection and metabolic reprogramming in Marsupenaeus japonicus

Integrated analysis of intestinal microbiota and metabolomic reveals that decapod iridescent virus 1 (DIV1) infection induces secondary bacterial infection and metabolic reprogramming in Marsupenaeus japonicus

The emerging pathogen Enterocytozoon hepatopenaei drives a degenerative cyclic pattern in the hepatopancreas microbiome of the shrimp (Penaeus vannamei)

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