White spot syndrome virus down-regulates expression of histones H2A and H4 of Penaeus vannamei to promote viral replication

Encinas‐García, Trinidad; Loreto-Quiroz, Daynette Lorely; Mendoza–Cano, Fernando; Peña‐Rodríguez, Alberto; Fimbres-Olivarria, Diana; Re-Vega, Enrique De La; Sánchez‐Paz, Arturo

doi:10.3354/dao03428

Cited by 6 publications

(2 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In human cells, H2A.Z acts as a negative regulator of antiviral responses via GCN5 and BRD2 ( 36 ). In shrimp Penaeus vannamei , the white spot syndrome virus (WSSV) decreased the expression of histones H2A and H4 to promote viral replication ( 37 ). Although the lysine rich histones H2A and H2B were less potent compared to the arginine rich histones H3, H3.3 and H4, all histones inhibited infectivity of influenza A H3N2 strain Phil82 in mammals ( 38 ).…”

Section: Discussionmentioning

confidence: 99%

Histone H2A Nuclear/Cytoplasmic Trafficking Is Essential for Negative Regulation of Antiviral Immune Response and Lysosomal Degradation of TBK1 and IRF3

Fang

Zhang

et al. 2021

Front. Immunol.

View full text Add to dashboard Cite

Histone H2A is a nuclear molecule tightly associated in the form of the nucleosome. Our previous studies have demonstrated the antibacterial property of piscine H2A variants against gram-negative bacteria Edwardsiella piscicida and Gram-positive bacteria Streptococcus agalactiae. In this study, we show the function and mechanism of piscine H2A in the negative regulation of RLR signaling pathway and host innate immune response against spring viremia of carp virus (SVCV) infection. SVCV infection significantly inhibits the expression of histone H2A during an early stage of infection, but induces the expression of histone H2A during the late stage of infection such as at 48 and 72 hpi. Under normal physiological conditions, histone H2A is nuclear-localized. However, SVCV infection promotes the migration of histone H2A from the nucleus to the cytoplasm. The in vivo studies revealed that histone H2A overexpression led to the increased expression of SVCV gene and decreased survival rate. The overexpression of histone H2A also significantly impaired the expression levels of those genes involved in RLR antiviral signaling pathway. Furthermore, histone H2A targeted TBK1 and IRF3 to promote their protein degradation via the lysosomal pathway and impair the formation of TBK1-IRF3 functional complex. Importantly, histone H2A completely abolished TBK1-mediated antiviral activity and enormously impaired the protein expression of IRF3, especially nuclear IRF3. Further analysis demonstrated that the inhibition of histone H2A nuclear/cytoplasmic trafficking could relieve the protein degradation of TBK1 and IRF3, and blocked the negative regulation of histone H2A on the SVCV infection. Collectively, our results suggest that histone H2A nuclear/cytoplasmic trafficking is essential for negative regulation of RLR signaling pathway and antiviral immune response in response to SVCV infection.

show abstract

Section: Discussionmentioning

confidence: 99%

Histone H2A Nuclear/Cytoplasmic Trafficking Is Essential for Negative Regulation of Antiviral Immune Response and Lysosomal Degradation of TBK1 and IRF3

Fang

Zhang

et al. 2021

Front. Immunol.

View full text Add to dashboard Cite

show abstract

“…Although histone deacetylation catalyzed by HDAC tightens the chromatin structure to silence gene expression ( 153 ), pathogens infection can disrupt this structure to inhibit host gene expression. For instance, the ICP11 protein of white spot syndrome virus (WSSV) can damage the nucleosome assembly in Penaeus vannamei , by binding to histone proteins to evade the hosts’ immune responses to promote viral replication ( 154 ).…”

Section: The Effect Of Metabolic and Epigenetic Modification On Train...mentioning

confidence: 99%

The mechanisms and factors that induce trained immunity in arthropods and mollusks

Zhao,

Lin,

Zheng

et al. 2023

Front. Immunol.

View full text Add to dashboard Cite

Besides dividing the organism’s immune system into adaptive and innate immunity, it has long been thought that only adaptive immunity can establish immune memory. However, many studies have shown that innate immunity can also build immunological memory through epigenetic reprogramming and modifications to resist pathogens’ reinfection, known as trained immunity. This paper reviews the role of mitochondrial metabolism and epigenetic modifications and describes the molecular foundation in the trained immunity of arthropods and mollusks. Mitochondrial metabolism and epigenetic modifications complement each other and play a key role in trained immunity.

show abstract

Susceptibility of Neohelice granulata (Decapoda, Varunidae) to white spot syndrome virus (WSSV)

Moser

Marques

2022

Aquacult Int

View full text Add to dashboard Cite

White Spot Syndrome Virus (WSSV) continues to be the most severe viral pathogen to the shrimp industry worldwide. Paci c white shrimp Litopenaeus vannamei is particularly affected by WSSV and this virus has been detected in a wide range of wild crustaceans, including penaeid and non-penaeid shrimp, cray sh, as well as crabs. Crabs have been considered as a dangerous threat to shrimp farms because they are generally believed to be highly tolerant to WSSV and to remain infected for long periods without signs of disease. The burrowing crab Neohelice (= Chasmagnathus) granulata can be commonly found in the surroundings of the shrimp farms in southern Brazil. Here, we investigated the susceptibility of N. granulata to WSSV infection in comparison to L. vannamei. WSSV infectability and host susceptibility were investigated by viral challenge (intramuscular injection) in both species. Viral load in challenged animals was quanti ed by qPCR in both hemolymph and gills. Furthermore, the transcript levels of sixteen target genes related to the molecular defense status were assessed. The results revealed that N. granulate experimentally infected by WSSV, as well as those naturally infected, showed lethargy, lack of appetite and later gross signs of the disease. Moreover, N. granulate showed to be less susceptible to WSSV when compared to L. vannamei. While no death was observed in crabs before a post-challenge interval of 120 h; shrimp death was seen as early as 48 hours post-infection. Comparative viral load was also assessed by qPCR in gills of captured wild crabs and farmed shrimp naturally infected by WSSV. Transcript levels of target genes were also investigated after WS-challenge between 24 and 48 hpi in shrimp and between 96 and 120 hpi in crab. Differences in gene transcripts were particularly noteworthy with the increase of viral copies at 10 6 copies.µl − 1 . These results indicated that WSSV infection modulated genes related of important cellular responses, such as apoptosis, chaperone function and ion storage, in both shrimp and crab.

show abstract

White spot syndrome virus down-regulates expression of histones H2A and H4 of Penaeus vannamei to promote viral replication

Cited by 6 publications

References 38 publications

Histone H2A Nuclear/Cytoplasmic Trafficking Is Essential for Negative Regulation of Antiviral Immune Response and Lysosomal Degradation of TBK1 and IRF3

Histone H2A Nuclear/Cytoplasmic Trafficking Is Essential for Negative Regulation of Antiviral Immune Response and Lysosomal Degradation of TBK1 and IRF3

The mechanisms and factors that induce trained immunity in arthropods and mollusks

Susceptibility of Neohelice granulata (Decapoda, Varunidae) to white spot syndrome virus (WSSV)

Contact Info

Product

Resources

About