White rice ethanol extract is qualitatively, but not quantitatively, equivalent to that of brown rice as an antioxidant source

Wu, Hongyan; Nakamura, Toshiyuki; Guo, Yuan‐ji; Hirooka, Miho; Zhang, Gongliang; Munemasa, Shintaro; Murata, Yoshiyuki; Fujita, Akiko; Nakamura, Yoshimasa

doi:10.1093/bbb/zbab133

Cited by 3 publications

(1 citation statement)

References 28 publications

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“…The expression of NFATc1 gene was determined by reversetranscription polymerase chain reaction (RT-PCR) as previously reported with some modifications. [24,25] After the pretreatment with BITC for 30 min, the cells were treated with RANKL (50 ng/ml) for 24 h in α-MEM. The total RNA was extracted using the TRIzol reagent (Thermo Fisher Scientific) according to the manufacturer's manual.…”

Section: Nuclear Factor Of Activated T Cells 1 (Nfatc1) Gene Expressionmentioning

confidence: 99%

Benzyl isothiocyanate and its metabolites inhibit cell proliferation through protein modification in mouse preosteoclast RAW264.7 cells

Nakamura

Tsutsui

Okuda

et al. 2022

J Biochem & Molecular Tox

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Benzyl isothiocyanate (BITC), derived from cruciferous vegetables, is an organosulfur compound exerting antiproliferative effects in several human cancer cells. In this study, we assessed BITC as a potential osteoclastogenesis inhibitor and investigated its underlying mechanism. BITC at 5 μM significantly decreased the viability of the osteoclast‐like differentiating RAW264.7 cells, coinciding with the downregulation of the primary biomarkers for osteoclast differentiation, such as the tartrate‐resistant acid phosphatase activity and nuclear factor of activated T‐cells gene expression. Not only BITC but also its metabolites, inhibited cell proliferation in the normal RAW264.7 cells, suggesting that BITC shows an anti‐osteoclastogenesis effect in vivo after its ingestion and metabolism, possibly through an antiproliferative action. Both BITC and its metabolites also enhanced the DNA fragmentation and the caspase‐3 activity, whereas their higher concentrations tended to suppress these effects. BITC was intracellularly accumulated when the cells were treated with its metabolites via their degradation into the free form. A quantitative experiment using the proteolysis/high performance liquid chromatography technique showed that the amount of BITC‐lysine thiourea in the cells was also increased in a time‐dependent manner, suggesting that lysine modification of the cellular proteins actually took place in the cells treated by BITC. Among the cellular proteins, the cleaved caspase‐3 was identified as a potential target for lysine modification by BITC. Taken together, BITC dissociated from its metabolites as well as its free form might modulate osteoclastogenesis, possibly through inhibition of cell proliferation by protein modification.

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Section: Nuclear Factor Of Activated T Cells 1 (Nfatc1) Gene Expressionmentioning

confidence: 99%

Benzyl isothiocyanate and its metabolites inhibit cell proliferation through protein modification in mouse preosteoclast RAW264.7 cells

Nakamura

Tsutsui

Okuda

et al. 2022

J Biochem & Molecular Tox

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Enhancing effect of the coexisting alpha-tocopherol on quercetin absorption and metabolism

Mitsuzane,

Okubo,

Nishikawa

et al. 2024

Free Radical Research

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Cycloartenyl Ferulate Is the Predominant Compound in Brown Rice Conferring Cytoprotective Potential against Oxidative Stress-Induced Cytotoxicity

Nakamura

Guo

et al. 2023

IJMS

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Since brown rice extract is a rich source of biologically active compounds, the present study is aimed to quantify the major compounds in brown rice and to compare their cytoprotective potential against oxidative stress. The content of the main hydrophobic compounds in brown rice followed the order of cycloartenyl ferulate (CAF) (89.00 ± 8.07 nmol/g) >> α-tocopherol (αT) (19.73 ± 2.28 nmol/g) > γ-tocotrienol (γT3) (18.24 ± 1.41 nmol/g) > α-tocotrienol (αT3) (16.02 ± 1.29 nmol/g) > γ-tocopherol (γT) (3.81 ± 0.40 nmol/g). However, the percent contribution of CAF to the radical scavenging activity of one gram of whole brown rice was similar to those of αT, αT3, and γT3 because of its weaker antioxidant activity. The CAF pretreatment displayed a significant cytoprotective effect on the hydrogen peroxide-induced cytotoxicity from 10 µM, which is lower than the minimal concentrations of αT and γT required for a significant protection. CAF also enhanced the nuclear factor erythroid 2-related factor 2 (Nrf2) nuclear translocation coincided with the enhancement of the heme oxygenase-1 (HO-1) mRNA level. An HO-1 inhibitor, tin protoporphyrin IX (SnPP), significantly impaired the cytoprotection of CAF. The cytoprotective potential of CAF is attributable to its cycloartenyl moiety besides the ferulyl moiety. These results suggested that CAF is the predominant cytoprotector in brown rice against hydrogen peroxide-induced cytotoxicity.

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White rice ethanol extract is qualitatively, but not quantitatively, equivalent to that of brown rice as an antioxidant source

Cited by 3 publications

References 28 publications

Benzyl isothiocyanate and its metabolites inhibit cell proliferation through protein modification in mouse preosteoclast RAW264.7 cells

Benzyl isothiocyanate and its metabolites inhibit cell proliferation through protein modification in mouse preosteoclast RAW264.7 cells

Enhancing effect of the coexisting alpha-tocopherol on quercetin absorption and metabolism

Cycloartenyl Ferulate Is the Predominant Compound in Brown Rice Conferring Cytoprotective Potential against Oxidative Stress-Induced Cytotoxicity

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