“…Hardness is defined as the slope of the f-d curve given in units of newtons per meter (N/m). Although the Park NX10 SPM system is capable of a 40 nm spatial resolution [3], a 300 nm spatial resolution was used in the interest of time. The system is also sensitive to changes in force as little as 60 pN and can detect up to 100 µN depending on the spring constant of the cantilever used.…”