White Matter Topographic Anatomy Applied to Temporal Lobe Surgery

Flores-Justa, Ana; Baldoncini, Matías; Cruz, Juan Carlos; González, Federico Sánchez; Martínez, Óscar; González-López, Pablo; Campero, Álvaro

doi:10.1016/j.wneu.2019.08.050

Cited by 15 publications

(14 citation statements)

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“…While no longer necessarily being viewed as a ground truth, the fiber dissection technique is nevertheless useful in the confirmation of DTI tractography and for the development of novel techniques (Dini et al, 2013;Zemmoura et al, 2014;De Benedictis et al, 2018). Although sometimes questioned, its usefulness in the field of neuroscience is confirmed by the fact that it is used in numerous recent studies (Di Carlo et al, 2019;Flores-Justa et al, 2019;Komaitis et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

White Matter Dissection of the Fetal Brain

et al. 2020

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Neuroplasticity is a complex process of structural and functional reorganization of brain tissue. In the fetal period, neuroplasticity plays an important role in the emergence and development of white matter tracts. Here, we aimed to study the architecture of normal fetal brains by way of Klingler's dissection. Ten normal brains were collected from in utero deceased fetuses aged between 13 and 35 gestational weeks (GW). During this period, we observed modifications in volume, shape, and sulci configuration. Our findings indicate that the major white matter tracts follow four waves of development. The first wave (13 GW) involves the corpus callosum, the fornix, the anterior commissure, and the uncinate fasciculus. In the second one (14 GW), the superior and inferior longitudinal fasciculi and the cingulum could be identified. The third wave (17 GW) concerns the internal capsule and in the fourth wave (20 GW) all the major tracts, including the inferioroccipital fasciculus, were depicted. Our results suggest an earlier development of the white matter tracts than estimated by DTI tractography studies. Correlating anatomical dissection with tractography data is of great interest for further research in the field of fetal brain mapping.

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Section: Introductionmentioning

confidence: 99%

White Matter Dissection of the Fetal Brain

et al. 2020

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“…Details of the technique, including fixation, freezing and thawing, are described in detail in Table 2, which summarizes our results (Baran et al 2019;Bozkurt et al 2017;Burks et al 2017;Capilla-Guasch et al 2019;Costa et al 2018;De Benedictis et al 2014;de Castro et al 2005;Di Carlo et al 2019;Dini et al 2013;Fernandez-Miranda et al 2008;Flores-Justa et al 2019;Goryainov et al 2017;Gungor et al 2018;Ludwig and Klingler 1956;Kadri et al 2017;Koutsarnakis et al 2015;Latini 2015;Latini et al 2015;Mandonnet et al 2017;Martino et al 2010;Panesar et al 2019;Pescatori et al 2017;Peuskens et al 2004;Rigoard et al 2011;Sarubbo et al 2016;Serra et al 2017;Shah et al 2012;Silva and Andrade 2016;Silva et al 2017;Sincoff Ture et al 2000;Vergani et al 2014;Verhaeghe et al 2018;Wang et al 2013;Wu et al 2016a, b;Yakar et al 2018).…”

Section: Resultsmentioning

confidence: 78%

“…Silva et al mentioned the importance of careful removal of the calvaria and described in detail their technique to avoid damage to the underlying dura and the brain (Silva and Andrade 2016). Some authors prefer to start fixation before the brain is removed from the skull, and this is performed with formalin solution, which is injected intraarterially (Latini et al 2015) (Silva and Andrade 2016;Silva et al 2017), (Flores-Justa et al 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Instruments initially used for dissection were Swiss watchmaker forceps and wooden spatulas, which are 2-4 mm wide, while metal spatulas were not recommended (Ludwig and Klingler 1956;Klingler and Gloor 1960). Most laboratories currently use wooden spatulas (Capilla-Guasch et al 2019;Costa et al 2018;De Benedictis et al 2014;de Castro et al 2005;Di Carlo et al 2019;Dini et al 2013;Flores-Justa et al 2019;Latini et al 2015;Panesar et al 2019;Pescatori et al 2017;Sarubbo et al 2016;Serra et al 2017;Shah et al 2012;Silva and Andrade 2016;Sincoff et al 2004;Ture et al 2000;Vergani et al 2014), but microsurgical metallic instruments (Capilla-Guasch et al 2019;Costa et al 2018;de Castro et al 2005;Flores-Justa et al 2019;Gungor et al 2018;Koutsarnakis et al 2015;Latini 2015;Latini et al 2015;Martino et al 2010;Peltier et al 2006;Peuskens et al 2004;Serra et al 2017;Silva and Andrade 2016;Vergani et al 2014;Wang et al 2013;Wu et al 2016b) have also been found to be useful for precise dissections. For a delicate dissection of low volume tracts, Klingler used snipe feathers, wet hair pencils or wet pieces of cotton wool (Klingler and Gl...…”

Section: Discussionmentioning

confidence: 99%

“…When following the methods section of manuscripts that include white matter dissection, most authors state that their technique is following Klingler's original technique (Capilla-Guasch et al 2019;De Benedictis et al 2014;Di Carlo et al 2019;Dini et al 2013;Fernandez-Miranda et al 2008;Flores-Justa et al 2019;Gungor et al 2018;Martino et al 2010;Peltier et al 2006;Peuskens et al 2004;Serra et al 2017;Shah et al 2012;Vergani et al 2014;Verhaeghe et al 2018;Wang et al 2013;Wu et al 2016a), or that their own modification (Burks et al 2017;de Castro et al 2005;Goryainov et al 2017;Latini et al 2015;Rigoard et al 2011;Silva and Andrade 2016;Sincoff et al 2004) of the Klingler's technique is applied. This may be confusing for those who want to start their own dissections.…”

Section: Introductionmentioning

confidence: 99%

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White matter dissection with the Klingler technique: a literature review

Dziedzic¹,

Balasa²,

Jeżewski³

et al. 2020

Brain Struct Funct

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The aim of this literature review is to present a summary of the published literature relating the details of the different modifications of specimen preparation for white matter dissection with the Klingler technique. For this review, 3 independent investigators performed an electronic literature search that was carried out in the Pubmed, Scopus and Web of Science databses up to December 2019. Furthermore, we performed citation tracking for the articles missed in the initial search. Studies were eligible for inclusion when they reported details of at least the first 2 main steps of Klingler’s technique: fixation and freezing. A total of 37 full-text articles were included in the analysis. We included original anatomical studies in which human white matter dissection was performed for study purposes. The main three steps of preparation are the same in each laboratory, but the details of each vary between studies. Ten percent formalin is the most commonly used (34 studies) solution for fixation. The freezing time varied between 8 h and a month, and the temperature varied from − 5 to − 80 °C. After thawing and during dissections, the specimens were most often kept in formalin solution (13), and the concentration varied from 4 to 10%. Klingler’s preparation technique involves three main steps: fixation, freezing and thawing. Even though the details of the technique are different in most of the studies, all provide subjectively good quality specimens for anatomical dissections and studies.

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