White Leaf and Panicle 2, encoding a PEP-associated protein, is required for chloroplast biogenesis under heat stress in rice

Lv, Yusong; Shao, Gaoneng; Qiu, Jian; Jiao, Guiai; Sheng, Zhonghua; Xie, Lihong; Wu, Yanjun; Tang, Shaoqing; Wei, Xiangjin

doi:10.1093/jxb/erx332

Cited by 51 publications

(60 citation statements)

References 41 publications

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“…5A, we found that all the parameters except intercellular CO 2 concentration (C i ) were reduced in the male sterile lines of S. miltiorrhiza. The results showed that the chloroplast structure was abnormal and the performance of photosynthesis was consistent with other research results [41].…”

Section: Discussionsupporting

confidence: 89%

Physiological and transcriptome analysis reveal molecular mechanism in Salvia miltiorrhiza leaves of near-isogenic male fertile lines and male sterile lines

Wang

Jiang

Zhou

et al. 2019

Preprint

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Background: Our previous study found that male sterility in Salvia miltiorrhiza could result in stunted growth, reduced biomass, decreased primary metabolism, and increased secondary metabolism, but their molecular mechanisms have not yet been revealed. In this article, we investigated the underlying mechanism of male sterility to plant growth and metabolic yield by using physiological analysis and mRNA sequencing (RNA-Seq). Results: In this study, transcriptomic and physiological analysis were performed to identify the mechanism of male sterile mutants to plant growth and metabolic yield. Through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, it was found that the pathways were mainly enriched in processes including organ development, primary metabolic process and secondary metabolic process. Physiological analysis showed that the chloroplast structure of male sterile mutants of S. miltiorrhiza was abnormally developed, which could result in decrease in leaf gas exchange (A, E and gs), chlorophyll fluorescence (Fv, Fm and Fv/Fm), and the chlorophyll content. Among the differentially expressed genes (DEGs), the expression levels of 7 genes involved to photosynthesis-related pathway were down-regulated in male sterile lines of S. miltiorrhiza, which was consistent with the corresponding phenotypic changes in chlorophyll fluorescence, chlorophyll content and leaf gas exchange. Transcriptomic analysis established the role of disproportionating enzyme 1 (DPE1) as catalyzing the degradation of starch, and the role sucrose synthase 3 (SUS3) and cytosolic invertase 2 (CINV2) as catalyzing the degradation of sucrose in the S. miltiorrhiza mutants. The results also confirmed that phenylalanine ammonialyase (PAL) was involved in the biosynthesis of rosmarinic acid and salvianolic acid B, and flavone synthase (FLS) was an important enzyme catalyzing steps of flavonoid biosynthesis. Conclusions: Our results from the physiological and transcriptome analysis reveal underlying mechanism of plant growth and metabolic yield in male sterile mutants, and provide insight into the crop yield of S. miltiorrhiza.

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Section: Discussionsupporting

confidence: 89%

Physiological and transcriptome analysis reveal molecular mechanism in Salvia miltiorrhiza leaves of near-isogenic male fertile lines and male sterile lines

Wang

Jiang

Zhou

et al. 2019

Preprint

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“…In this paper, we measured the photosynthetic parameters of S. miltiorrhiza leaves of the male fertile lines and male sterile lines, and found that all the parameters including the net photosynthetic rate (A), transpiration rate (E) and stomatal conductance (g s ) of the male fertile lines were higher than those of the male sterile lines except intercellular CO 2 concentration (C i ) in S. miltiorrhiza ( Figure 5A). The results showed that the chloroplast structure was abnormal and the performance of photosynthesis was consistent with other research results [20]. The results also showed that the net photosynthetic rate was positively correlated with the stomatal conductance, which agrees with the statistical analysis of 14 species [21].…”

Section: Abnormal Development Of Chloroplast Structure In Male Sterilsupporting

confidence: 90%

Physiological and transcriptome analysis reveal molecular mechanism in Salvia miltiorrhiza leaves of near-isogenic male fertile lines and male sterile lines

Wang

Guo

Dong

2019

Preprint

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Background: Our previous study found that male sterility in Salvia miltiorrhiza could result in stunted growth, decrease biomass, inhibit primary metabolism, and promote secondary metabolism, but their molecular mechanisms have not yet been elucidated. In this article, we investigated the underlying mechanism of plant growth and metabolism by using physiological analysis and mRNA sequencing (RNA-Seq). Results: In this study, transcriptomic and physiological analyses were performed to identify the effect on plant growth and metabolic production in male sterile mutants. Through GO and KEGG analysis it was found that the pathways were mainly enriched in processes including organ development, primary metabolic process and secondary metabolic process. Physiological analyses showed that the chloroplast structure of male sterile mutants of Salvia miltiorrhiza was abnormally developed, which could result in decrease in leaf gas exchange (A, E and gs), chlorophyll fluorescence (Fv, Fm and Fv/Fm), and the chlorophyll content. Transcriptomic analyses indicated that disproportionating enzyme 1 (DPE1) catalyzed the degradation of starch, while sucrose synthase 3 (SUS3) and cytosolic invertase 2 (CINV2) catalyzed the degradation of sucrose in S. miltiorrhiza. The results suggested that phenylalanine ammonialyase (PAL) played an important role in the biosynthesis of rosmarinic acid and salvianolic acid B, and flavone synthase (FLS) was an important enzyme catalyzing steps of flavonoid biosynthesis. High expression level of these enzyme genes in male sterile mutants resulted in high content of secondary metabolites. Conclusions: Our results from the physiological and transcriptome analyses reveal underlying mechanism of plant growth and metabolism in male sterile mutants, and provide insight into the crop production of S. miltiorrhiza.

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“…To investigate if plastid transcription was impaired by the mutation of UMPK , the expression of plastid genes was examined in young leaves of six-week-old WT and mutant plants. The genes psaA , psbB , psbC, petB , and rbcL were selected as plastid-encoded RNA polymerase (PEP)-dependent genes (Figure 3a); rpoA , rpoB , rpoC1 , and rpl23 were selected as nucleus-encoded RNA polymerase (NEP)-dependent genes (Figure 3b); atpB , ndhB , and psbE were chosen as both PEP- and NEP-dependent genes (Figure 3c) [23,24,25,26]. The expression of all assayed PEP-dependent genes was significantly lower in the mutant, except for rbcL , which showed no significant difference between the WT and the mutant.…”

Section: Resultsmentioning

confidence: 99%

“…The results showed that transcript levels of PEP-dependent genes and NEP-dependent genes in the umpk mutant were significantly reduced and elevated, respectively, compared with WT and the complementation lines. This is a typical plastid gene expression pattern resulting from impaired PEP transcription, which frequently results in abnormal chloroplast development [23,26,32,33,34,35,36,37]. The influence of impaired PEP activity on chloroplast development varied during the development of plants: it was more severe at the early stage and gradually diminished with plant development.…”

Section: Discussionmentioning

confidence: 99%

UMP Kinase Regulates Chloroplast Development and Cold Response in Rice

Dong

Zhang

Zhou

et al. 2019

IJMS

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Pyrimidine nucleotides are important metabolites that are building blocks of nucleic acids, which participate in various aspects of plant development. Only a few genes involved in pyrimidine metabolism have been identified in rice and the majority of their functions remain unclear. In this study, we used a map-based cloning strategy to isolate a UMPK gene in rice, encoding the UMP kinase that phosphorylates UMP to form UDP, from a recessive mutant with pale-green leaves. In the mutant, UDP content always decreased, while UTP content fluctuated with the development of leaves. Mutation of UMPK reduced chlorophyll contents and decreased photosynthetic capacity. In the mutant, transcription of plastid-encoded RNA polymerase-dependent genes, including psaA, psbB, psbC and petB, was significantly reduced, whereas transcription of nuclear-encoded RNA polymerase-dependent genes, including rpoA, rpoB, rpoC1, and rpl23, was elevated. The expression of UMPK was significantly induced by various stresses, including cold, heat, and drought. Increased sensitivity to cold stress was observed in the mutant, based on the survival rate and malondialdehyde content. High accumulation of hydrogen peroxide was found in the mutant, which was enhanced by cold treatment. Our results indicate that the UMP kinase gene plays important roles in regulating chloroplast development and stress response in rice.

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White Leaf and Panicle 2, encoding a PEP-associated protein, is required for chloroplast biogenesis under heat stress in rice

Abstract: WLP2, encoding a PEP-associated protein, and its paralog OsFLN2 can interact with OsTRXz to form a TRX-FLN regulatory module to protect chloroplast development from heat stress in rice.

Cited by 51 publications

References 41 publications

Physiological and transcriptome analysis reveal molecular mechanism in Salvia miltiorrhiza leaves of near-isogenic male fertile lines and male sterile lines

Physiological and transcriptome analysis reveal molecular mechanism in Salvia miltiorrhiza leaves of near-isogenic male fertile lines and male sterile lines

Physiological and transcriptome analysis reveal molecular mechanism in Salvia miltiorrhiza leaves of near-isogenic male fertile lines and male sterile lines

UMP Kinase Regulates Chloroplast Development and Cold Response in Rice

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