White‐fruited <i>Duchesnea indica</i> (Rosaceae) is impaired in <i>ANS</i> gene expression

Debes, Mario Alberto; Arias, Marta E.; Grellet-Bournonville, Carlos F.; Wulff, Arturo F.; Martínez‐Zamora, Martin Gustavo; Castagnaro, Atilio Pedro; Ricci, Juan C. Díaz

doi:10.3732/ajb.1000373

Cited by 22 publications

(19 citation statements)

References 32 publications

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“…We assume that slight expression of PgANS is insufficient to produce anthocyanidins that could block anthocyanin accumulation. Similar results have been reported by Kim et al (2004Kim et al ( , 2005, Debes et al (2011), andJaakola et al (2002), who reported that restrictive expression of the ANS gene was responsible for the absence of anthocyanin accumulation.…”

Section: Gene Expression In Pomegranatesupporting

confidence: 90%

“…5, the PgMYB gene was expressed in both red and white pomegranate fruits. The MYB10 gene has been similarly found to be expressed in red and white genotypes of Duchesnea indica (Debes et al 2011). In contrast, the uniform expression of PgMYB observed in our study differs from results reported by Espley et al (2007) for apple, Kotepong et al (2011) for Malay apple, andLin-Wang et al (2010) for fruits of F. × ananassa and F. vesca.…”

Section: Gene Expression In Pomegranatecontrasting

confidence: 85%

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Cloning and expression of anthocyanin biosynthetic genes in red and white pomegranate

et al. 2015

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Exterior fruit color is an important trait for the evaluation of pomegranate fruit quality, but the molecular mechanism underlying the variation in color between red- and white-fruited pomegranate is poorly understood. In this study, full-length cDNA clones encoding enzymes involved in anthocyanin biosynthesis-such as chalcone synthase, chalcone isomerase, flavanone 3-hydoxylase, dihydroflavonol 4-reductase, anthocyanidin synthase (ANS), UDP-glucose-flavonoid 3-O-glucosyltransferase, and the R2R3 MYB transcription factor PgMYB-were isolated from fruit peels. In addition, transcript levels of anthocyanin biosynthetic genes were quantitatively measured by real-time PCR in red and white fruits. In both cultivars, two expression peaks for structural genes were detected during fruit development, whereas only one peak was observed-during early development-for PgMYB. While PgMYB is important for flavonoid biosynthesis, other transcription factors appear to also be necessary for the regulation of anthocyanin biosynthesis. No anthocyanins were detected in the white cultivar. Peels of white fruits contained transcripts of all identified genes except for PgANS, suggesting that the lack of PgANS expression may be the main factor responsible for the absence of anthocyanins in white pomegranate. PgANS may be the key gene involved in anthocyanin biosynthesis in pomegranate fruit.

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Section: Gene Expression In Pomegranatesupporting

confidence: 90%

Section: Gene Expression In Pomegranatecontrasting

confidence: 85%

Cloning and expression of anthocyanin biosynthetic genes in red and white pomegranate

et al. 2015

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“…In a tobacco model, Aharoni et al (2001) reported that FaMYB1 overexpression is associated with a decreased ANS expression and FGT activity leading to a reduced amount of anthocyanins in flowers, which is consistent with our results. Furthermore, an impairment of ANS and FGT genes causes anthocyanin content reductions in strawberry and other plant species (Kim et al 2005, Griesser et al 2008, Debes et al 2011, Zhao et al 2012.…”

Section: Discussionmentioning

confidence: 99%

RNAi-mediated silencing and overexpression of the FaMYB1 gene and its effect on anthocyanin accumulation in strawberry fruit

Kadomura-Ishikawa¹,

Miyawaki²,

Takahashi³

et al. 2015

Biol. plant.

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Strawberry (Fragaria × ananassa) contains anthocyanins, which are important secondary metabolites and key contributors to the antioxidant capacity and nutritional value of the fruit. Anthocyanin biosynthetic genes have been identified. However, the detailed mechanism responsible for anthocyanin accumulation and regulation of biosynthetic genes during strawberry fruit ripening remain unclear. In the present study, we examined the effect of a Fragaria × ananassa myeloblastosis 1 homolog, FaMYB1, on anthocyanin accumulation in the strawberry fruit receptacle. Expression analysis shows that FaMYB1 transcripts increased in response to irradiance but not to abscisic acid treatments. Down-regulation of FaMYB1 was achieved in planta using Agrobacterium-mediated RNA interference (RNAi). As a result, FaMYB1-RNAi fruits exhibited a significant increase in anthocyanin content. Conversely, overexpression of FaMYB1 resulted in a decrease in anthocyanin content. Overexpression of FaMYB1 also significantly reduced expression of genes encoding anthocyanidin synthase and flavonoid glycosyltransferase, whereas downregulation of FaMYB1 resulted in a significant decrease in the amount of transcripts of leucoanthocyanidin reductase. These data suggest that FaMYB1 might negatively control anthocyanin biosynthesis in the strawberry fruit at the branching-point of anthocyanin/proanthocyanidin biosynthesis.

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“…Furthermore, few changes of achenescolor of white-fruited D. indica were observed seasonally but never in the red-fruited populations. Successful crosses between D. indica f. indica and D. indica f. albocaput in both directions and self-pollinations were carried out by Debes et al (2011); those authors report compatibility between them and variations in color of fruiting-receptacle and achenes in offspring.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, mutations in genes encoding for the biosynthetic or transcriptional regulators of the anthocyanin pathway have been linked to changes in color phenotypes (Espley et al, 2009). Molecular studies in red-and whitefruited D. indica reported that impaired in ANS gene expression is responsible of white color of fruits (Debes et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

First report of Duchesnea indica f. albocaput (Rosaceae) in Northwestern Argentina.

Debes¹,

Orce²,

Luque³

et al. 2018

Bol. Soc. Argent. Bot.

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White‐fruited Duchesnea indica (Rosaceae) is impaired in ANS gene expression

Abstract: Results obtained indicate that the white-fruited character was stable. Mother progenitors exert a strong influence on the expression of the white-fruited character. The white-fruited phenotype is due to the impairment or downregulation of the ANS gene.

Cited by 22 publications

References 32 publications

Cloning and expression of anthocyanin biosynthetic genes in red and white pomegranate

Cloning and expression of anthocyanin biosynthetic genes in red and white pomegranate

RNAi-mediated silencing and overexpression of the FaMYB1 gene and its effect on anthocyanin accumulation in strawberry fruit

First report of Duchesnea indica f. albocaput (Rosaceae) in Northwestern Argentina.

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