White biotechnology: State of the art strategies for the development of biocatalysts for biorefining

Heux, Stéphanie; Meynial-Salles, Isabelle; O’Donohue, Michael; Dumon, Claire

doi:10.1016/j.biotechadv.2015.08.004

Cited by 92 publications

(51 citation statements)

References 282 publications

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“…Technologies for the rapid screening of GH’s activities are currently in development for high throughput analysis [6, 11, 38, 65]. Functional metagenomic has been proven to be useful tool to achieve this screening of GH’s activities (see review [27, 58]). However, like any screening related technology they face the paradigm “you get what you screened for”.…”

Section: Discussionmentioning

confidence: 99%

CAZyChip: dynamic assessment of exploration of glycoside hydrolases in microbial ecosystems

et al. 2016

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BackgroundMicroorganisms constitute a reservoir of enzymes involved in environmental carbon cycling and degradation of plant polysaccharides through their production of a vast variety of Glycoside Hydrolases (GH). The CAZyChip was developed to allow a rapid characterization at transcriptomic level of these GHs and to identify enzymes acting on hydrolysis of polysaccharides or glycans.ResultsThis DNA biochip contains the signature of 55,220 bacterial GHs available in the CAZy database. Probes were designed using two softwares, and microarrays were directly synthesized using the in situ ink-jet technology. CAZyChip specificity and reproducibility was validated by hybridization of known GHs RNA extracted from recombinant E. coli strains, which were previously identified by a functional metagenomic approach. The GHs arsenal was also studied in bioprocess conditions using rumen derived microbiota.ConclusionsThe CAZyChip appears to be a user friendly tool for profiling the expression of a large variety of GHs. It can be used to study temporal variations of functional diversity, thereby facilitating the identification of new efficient candidates for enzymatic conversions from various ecosystems.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-2988-4) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

CAZyChip: dynamic assessment of exploration of glycoside hydrolases in microbial ecosystems

et al. 2016

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“…Although these chemicals are presently derived from petroleum, they can be sourced from lignin at higher atom economies. The development of conversion platforms that can efficiently valorize lignin is highly desired, particularly biocatalytic processes that are greener, emit lesser CO 2 and are more energy efficient than thermocatalytic alternatives. [8][9][10][11][12][13] To this end, functional metagenomics is poised to be a major driver of the discovery of superior biocatalysts for the valorization of lignin.…”

Section: Introductionmentioning

confidence: 99%

Metagenomic discovery of a novel transaminase for valorization of monoaromatic compounds

2018

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The profitability of next-generation biorefineries is acutely contingent on the discovery and utilization of biocatalysts that can valorize lignin. To this end, the metabolic catalogues of diverse microbiota have been mined previously using functional metagenomics in order to identify biocatalysts that can selectively degrade lignin into monoaromatic compounds. Herein, we have further improved the valorization factor of biorefining by deploying functional metagenomics toward the identification of a novel transaminase that can selectively functionalize lignin-derived monoaromatics to produce value-added feedstocks for pharmaceutical synthesis.We implemented a high-throughput colorimetric assay using o-xylylenediamine as the amino donor and successfully identified a transaminase that utilizes the canonical cofactor, pyridoxal 5 0 -phosphate, to aminate as many as 14 monoaromatic aldehydes and ketones. We subsequently identified the optimal conditions for enzyme activity towards the most favoured amino acceptor, benzaldehyde, including temperature, pH and choice of co-solvent. We also evaluated the specificity of the enzyme towards a variety of amino donors, as well as the optimal concentration of the most favoured amino donor. Significantly, the novel enzyme is markedly smaller than typical transaminases, and it is stably expressed in E. coli without any modifications to its amino acid sequence. Finally, we developed and implemented a computational methodology to assess the activity of the novel transaminase. The methodology is generalizable for assessing any transaminase and facilitates in silico screening of enzyme-substrate combinations in order to develop efficient biocatalytic routes to value-added amines. The computational pipeline is an ideal complement to metagenomics and opens new possibilities for biocatalyst discovery.

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“…Biotechnological processes offer the opportunity to redesign traditional industrial practices through implementation of new and more sustainable approaches [4][5][6][7]. For instance, fermentation technology was key to the development of first and second generation biofuels (i.e.…”

Section: Introductionmentioning

confidence: 99%

Microalgae biorefineries: applications and emerging technologies

Giraldo

Romo-Buchelly

Arbeláez-Pérez

et al. 2018

DYNA

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Microalgae transform CO2 into a broad portfolio of biomolecules, and thus should be considered as a valuable biotechnological platform. Despite several research programs and global efforts to establish a sustainable microalgae-based industry, most applications have not transcended academic boundaries. This limitation raises from the high transformation costs when target products are biofuels or fertilizers. Microalgae biorefinery emerges as an alternative to improve economic competitiveness, as Under such a model scope, the process inputs come from industrial waste, while biomass exploitation prioritizes the highest value molecules followed by extraction of less valuable compounds. This review describes a wide range of microalgae exploitation schemes focused on new uses of its constituents, while discussing emerging technologies designed to improve production and efficiencies as well.Keywords: microalgae; biorefinery; biofuels; fertilizers; active biomolecules. Biorefinerías de microalgas: aplicaciones actuales y nuevas tecnologías en desarrolloResumen Las microalgas transforman el CO2 en un amplio portafolio de biomoléculas, por lo cual, son consideradas una valiosa plataforma biotecnológica. A pesar de múltiples programas de investigación y esfuerzos globales para establecer una industria sostenible basada en microalgas, la mayoría de las aplicaciones potenciales no han trascendido las fronteras académicas. Esta limitación se debe a los altos costos en la transformación del producto principalmente cuando se obtiene compuestos económicos como biocombustibles y fertilizantes. La biorefinería de microalgas surge como alternativa para incrementar la competitividad económica. En este modelo, los insumos del proceso provienen de residuos industriales, mientras que la explotación de la biomasa inicia con las moléculas de alto valor y finaliza con los compuestos menos valiosos. En esta revisión se describe un amplio abanico de esquemas de explotación de microalgas enfocado en nuevos usos de sus constituyentes. Además, se exploran las tecnologías emergentes destinadas a aprovechar esta biomasa de una manera más versátil y eficiente.

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White biotechnology: State of the art strategies for the development of biocatalysts for biorefining

Cited by 92 publications

References 282 publications

CAZyChip: dynamic assessment of exploration of glycoside hydrolases in microbial ecosystems

CAZyChip: dynamic assessment of exploration of glycoside hydrolases in microbial ecosystems

Metagenomic discovery of a novel transaminase for valorization of monoaromatic compounds

Microalgae biorefineries: applications and emerging technologies

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