Whisker-signaled Eyeblink Classical Conditioning in Head-fixed Mice

Lin, Carmen; Disterhoft, John F.; Weiss, Craig

doi:10.3791/53310

Cited by 8 publications

(8 citation statements)

References 30 publications

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“…There were six behavioral groups: Young Naive (n = 40 animals), Adult Naive (n = 30), Young Conditioned (n = 21), Young Pseudoconditioned (n = 19), Aged Unimpaired (n = 21), and Aged Impaired (n = 16). Young Conditioned, Young Pseudoconditioned, Aged Unimpaired, and Aged Impaired rats were trained on trace eyeblink conditioning as previously described ( Knuttinen et al, 2001 ; McKay et al, 2012 ; McKay et al, 2013 ; Lin et al, 2016 ). Aged Unimpaired and Aged Impaired were separated into their groups based on whether they reached a 60% conditioned response learning criterion.…”

Section: Methodsmentioning

confidence: 99%

“…Young Pseudoconditioned rats were given 30 unpaired CS alone trials pseudo-randomly dispersed with 30 unpaired US alone trials with a 10 to 20 s intertrial interval (15 s average). Stimuli were delivered and data were acquired and analyzed using custom LabView software ( https://github.com/JPow2020/Eyeblink-Conditioning , Power, 2011 , copy archived at https://github.com/elifesciences-publications/Eyeblink-Conditioning ; McKay et al, 2013 ; Lin et al, 2016 ).…”

Section: Methodsmentioning

confidence: 99%

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Persistent firing in LEC III neurons is differentially modulated by learning and aging

Lin

Sherathiya

et al. 2020

eLife

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Whether and how persistent firing in lateral entorhinal cortex layer III (LEC III) supports temporal associative learning is still unknown. In this study, persistent firing was evoked in vitro from LEC III neurons from young and aged rats that were behaviorally naive or trained on trace eyeblink conditioning. Persistent firing ability from neurons from behaviorally naive aged rats was lower compared to neurons from young rats. Neurons from learning impaired aged animals also exhibited reduced persistent firing capacity, which may contribute to aging-related learning impairments. Successful acquisition of the trace eyeblink task, however, increased persistent firing ability in both young and aged rats. These changes in persistent firing ability are due to changes to the afterdepolarization, which may in turn be modulated by the postburst afterhyperpolarization. Together, these data indicate that successful learning increases persistent firing ability and decreases in persistent firing ability contribute to learning impairments in aging.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Persistent firing in LEC III neurons is differentially modulated by learning and aging

Lin

Sherathiya

et al. 2020

eLife

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“…The training apparatus included a cylindrical treadmill inside a sound attenuating training chamber (IAC Acoustics). Mice were head fixed and allowed to run freely on the cylindrical treadmill (see Lin et al, 2016). Mice were allowed to habituate to the chamber and cylindrical treadmill during two separate sessions the day before training.…”

Section: Delay Eyeblink Conditioningmentioning

confidence: 99%

Intrinsic Excitability Increase in Cerebellar Purkinje Cells Following Delay Eyeblink Conditioning in Mice

Titley

Watkins

Lin

et al. 2018

Preprint

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Cerebellar learning is canonically thought to rely on synaptic plasticity, particularly at synaptic inputs to Purkinje cells. Recently, however, other complementary mechanisms have been identified. Intrinsic plasticity is one such mechanism, and depends in part on the down-regulation of calcium-dependent SK-type K channels, which is associated with an increase in neuronal excitability. In the hippocampus, SK-mediated intrinsic plasticity has been shown to play a role in trace eyeblink conditioning; however, it is not yet known how intrinsic plasticity contributes to a cerebellar learning task such as delay eyeblink conditioning. Whole cell recordings were obtained from acute cerebellar slices from mice ~48 hours after learning a delay eyeblink conditioning task. Over a period of repeated training sessions mice received either distinctly paired trials of a tone co-terminating with a periorbital shock (conditioned mice) or trials in which these stimuli were presented in an unpaired manner (pseudoconditioned mice). Conditioned mice show a significantly reduced afterhyperpolarization (AHP) following trains of parallel fiber stimuli.Moreover, we find that SK-dependent intrinsic plasticity is occluded in conditioned, but not pseudoconditioned mice. These findings show that excitability is enhanced in Purkinje cells after delay eyeblink conditioning and point toward a downregulation of SK channels as a potential underlying mechanism.

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“…We prepared the skull as usual but then applied a layer of C & B Metabond cement (Parkell, Brentwood, NY) and then covered the cured Metabond with Grip Cement. Metabond was chosen because it is being used by several laboratories to secure anchors to the skull of head-fixed behaving mice (Lin et al, 2016; Chettih et al, 2011; Dombeck & Tank, 2014). The headbolts stayed securely on the head for several weeks in 9 of 12 rabbits.…”

Section: Surgical Implantation Of the Headboltmentioning

confidence: 99%