Which Yeast Species Shall I Choose? Saccharomyces cerevisiae Versus Pichia pastoris (Review)

Darby, Richard A. J.; Cartwright, Stephanie P.; Dilworth, Marvin V.; Bill, Roslyn M.

doi:10.1007/978-1-61779-770-5_2

Cited by 60 publications

(39 citation statements)

References 65 publications

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“…These factors may be less differentiated cells and plastids (53). The recombinant protein secreted to the culture medium may be un-stable.…”

Section: Determination Of the Protein Content And Activity Assay Of Pmentioning

confidence: 99%

Biotechnological Production of Recombinant Tissue Plasminogen Activator Protein , its Application

Kumar¹,

Tjprc²

2018

IJBTR

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“…These factors may be less differentiated cells and plastids (53). The recombinant protein secreted to the culture medium may be un-stable.…”

Section: Determination Of the Protein Content And Activity Assay Of Pmentioning

confidence: 99%

Biotechnological Production of Recombinant Tissue Plasminogen Activator Protein , its Application

Kumar¹,

Tjprc²

2018

IJBTR

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“…Flask fermentations were performed under anaerobic conditions using optimal minimal medium (OMM), which was a modified version of previous defined media compositions [89,90], and contained (per liter) 1.7 g yeast nitrogen base (YNB) lacking ammonium sulfate (catalog number Y1251; Sigma-Aldrich, St. Louis, MO, USA), two-fold appropriate CSM dropout mixture (MP Biomedicals, Santa Ana, CA, USA), 10 g (NH4) 2 SO 4 , 1 g MgSO 4 · 7H 2 O, 6 g KH 2 PO 4 , 100 mg adenine hemisulfate, 10 mg inositol, as well as an additional 100 mg glutamic acid, 20 mg lysine, and 375 mg serine. In addition, 80 g/l of glucose, 80 g/l cellobiose or 40 g/l xylose were added as carbon sources.…”

Section: Anaerobic Fermentationmentioning

confidence: 99%

Leveraging transcription factors to speed cellobiose fermentation by

Lin

Chomvong

Acosta-Sampson

et al. 2014

Biotechnol Biofuels

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Background: Saccharomyces cerevisiae, a key organism used for the manufacture of renewable fuels and chemicals, has been engineered to utilize non-native sugars derived from plant cell walls, such as cellobiose and xylose. However, the rates and efficiencies of these non-native sugar fermentations pale in comparison with those of glucose. Systems biology methods, used to understand biological networks, hold promise for rational microbial strain development in metabolic engineering. Here, we present a systematic strategy for optimizing non-native sugar fermentation by recombinant S. cerevisiae, using cellobiose as a model. Results: Differences in gene expression between cellobiose and glucose metabolism revealed by RNA deep sequencing indicated that cellobiose metabolism induces mitochondrial activation and reduces amino acid biosynthesis under fermentation conditions. Furthermore, glucose-sensing and signaling pathways and their target genes, including the cAMP-dependent protein kinase A pathway controlling the majority of glucose-induced changes, the Snf3-Rgt2-Rgt1 pathway regulating hexose transport, and the Snf1-Mig1 glucose repression pathway, were at most only partially activated under cellobiose conditions. To separate correlations from causative effects, the expression levels of 19 transcription factors perturbed under cellobiose conditions were modulated, and the three strongest promoters under cellobiose conditions were applied to fine-tune expression of the heterologous cellobiose-utilizing pathway. Of the changes in these 19 transcription factors, only overexpression of SUT1 or deletion of HAP4 consistently improved cellobiose fermentation. SUT1 overexpression and HAP4 deletion were not synergistic, suggesting that SUT1 and HAP4 may regulate overlapping genes important for improved cellobiose fermentation. Transcription factor modulation coupled with rational tuning of the cellobiose consumption pathway significantly improved cellobiose fermentation.

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“…In the first phase, respiro-fermentative Sugarcane Bagasse as a Carrier for the Immobilization phase, sugar was converted into ethanol. Then ethanol, in the second phase, was consumed to produce carbon dioxide and water [33]. Ethanol is very easy to be used as carbon sources by the yeast therefore not through an active transport mechanism.…”

Section: Immobilization Of Yeast Cells To Sugarcane Bagassementioning

confidence: 99%

“…This is due to the cells density in higher biocatalyst were more numerous. When the cells density was high, the sugar utilization as carbon sources would also increase [33].…”

Section: Immobilization Of Yeast Cells To Sugarcane Bagassementioning

confidence: 99%

Sugarcane Bagasse as a Carrier for the Immobilization of Saccharomyces cerevisiaein Bioethanol Production

Anita¹,

Mangunwardoyo²,

Yopi³

2016

MST

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Which Yeast Species Shall I Choose? Saccharomyces cerevisiae Versus Pichia pastoris (Review)

Cited by 60 publications

References 65 publications

Biotechnological Production of Recombinant Tissue Plasminogen Activator Protein , its Application

Biotechnological Production of Recombinant Tissue Plasminogen Activator Protein , its Application

Leveraging transcription factors to speed cellobiose fermentation by

Sugarcane Bagasse as a Carrier for the Immobilization of Saccharomyces cerevisiaein Bioethanol Production

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