Which proliferation markers for routine immunohistology? A comparison of five antibodies.

Rose, D. S. C.; Maddox, P.; Brown, D. C.

doi:10.1136/jcp.47.11.1010

Cited by 105 publications

(66 citation statements)

References 38 publications

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“…MIB-1 is a monoclonal antibody recognizing Ki-67 nuclear nonhistone protein expressed throughout the mitotic cycle, with the exception of the G 0 phase. Immunostaining with MIB-1 or polyclonal anti-Ki-67 antibodies yields the same results (17). In normal vulvar skin and vulvar/cervical mucosa, MIB-1 positivity is found exclusively in the parabasal nuclei of the squamous epithelium (3, 5, 18 -22).…”

Section: Discussionsupporting

confidence: 62%

Low-Grade Vulvar and Vaginal Intraepithelial Neoplasia: Correlation of Histologic Features with Human Papillomavirus DNA Detection and MIB-1 Immunostaining

Logani¹,

Quint

et al. 2003

Modern Pathology

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Histologic criteria of low-grade vulvar/vaginal intraepithelial neoplasia (VIN1/VAIN1) are well established; however, a significant interobserver variability in diagnosing VIN1/VAIN1 has been reported. The goal of this study was to evaluate the utility of MIB-1 immunostaining as an adjunct test to increase the diagnostic accuracy in equivocal cases of VIN1/VAIN1. The second goal was to examine the distribution of low-and high-oncogenic risk human papillomaviruses (HPVs) in VIN1/VAIN1 lesions. Consecutive vulvar/vaginal biopsies originally diagnosed as VIN1/VAIN1 (n ‫؍‬ 43) or benign (n ‫؍‬ 20) were reviewed by two pathologists to obtain a consensus diagnosis. The diagnosis was further confirmed with HPV testing using Short PCR Fragment 10 and Line Probe Assay. MIB-1 immunostaining was performed, and positive staining was defined as a cluster of two or more stained nuclei in the upper two thirds of the epithelial thickness. After verification of the diagnosis using the consensus histologic review and HPV detection as an objective confirmatory test, 31% of cases originally diagnosed as VIN1/VAIN1 were identified as being overdiagnosed. The sensitivity and the specificity of MIB-1 staining for identifying VIN1/VAIN1 were 0.96 and 0.90, respectively. Seventy percent of VIN1 cases were associated with low-risk viral types. In contrast, the majority (84%) of VAIN1 cases were associated with high-risk HPVs. In conclusion, MIB-1 staining is sensitive and specific for identifying VIN1/VAIN1, helpful in verifying the diagnosis in equivocal cases.

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Section: Discussionsupporting

confidence: 62%

Low-Grade Vulvar and Vaginal Intraepithelial Neoplasia: Correlation of Histologic Features with Human Papillomavirus DNA Detection and MIB-1 Immunostaining

Logani¹,

Quint

et al. 2003

Modern Pathology

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“…Rose et al found a qualitatively similar immune staining using MIB-1 and polyclonal Ki-67 antibodies, but quantitative comparisons were not performed. 23 To our knowledge, the only previous study that has presented relevant quantitative data is the study of Torp in 1997. 47 He determined Ki-67 LIs in 11 glioblastomas by staining with the prototypic Ki-67 antibody in addition to the same four Ki-67 equivalent antibodies that were used in our Table 2 Ki-67 labelling indices in tonsillar squamous epithelium from 10 cases stained with four different Ki-67 equivalent antibodies study.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Ki-67 equivalent antibodies

Lindboe¹,

Torp²

2002

Journal of Clinical Pathology

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Aims: To compare commercially available Ki-67 equivalent antibodies with regard to qualitative and quantitative immunohistochemical staining characteristics. Methods: The following antibodies were used: monoclonal MIB-1 (Immunotech), monoclonal MM1 (Novocastra), polyclonal NCL-Ki-67p (Novocastra), and polyclonal Rah Ki-67 (Dako). All immunostainings were evaluated in squamous epithelium from formalin fixed and paraffin wax embedded pharyngeal tonsils. Labelling indices (LIs) were recorded twice to test their reproducibility. Results: By application of all four antibodies the nuclear staining could be either diffuse, granular, or a combination of both (classified as granular in this study). The diffuse pattern generally showed a strong or moderate staining intensity, whereas the granular pattern displayed a continuum from strong to very weak, making it difficult to discriminate between positive and negative nuclei. The diffuse staining pattern was seen in approximately 59% of the nuclei with the MIB-1 antibody and in 35-45% when the other antibodies were used. The following mean LIs were recorded: MIB-1, 31%; NCL-Ki-67p, 21%; Rah Ki-67, 17%; and MM1, 14%. The reproducibility was excellent for all four antibodies, with the mean of differences between the two runs of counts ranging from 1.1% to 1.5%. Conclusions: The four tested Ki-67 equivalent antibodies revealed differences in qualitative and quantitative staining characteristics, which resulted in considerable variations in registered LIs. The MIB-1 antibody appears to have a higher sensitivity for detecting the Ki-67 antigen than the other three tested antibodies. These differences are important to consider when proliferative activity is determined by the Ki-67 LI.

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“…The prognostic value of the Ki-67 proliferative tumor marker as assessed by the MIB-1 antibody is well established in several types of tumors, e.g., squamous cell carcinoma of the esophagus, non-Hodgkin's lymphoma, non-small cell lung cancer, cervical cancer, pancreatic head cancer, prostatic cancer (7,(25)(26)(27)(28)(29), as well as by Delahunt in Wilms' tumor (11). In the present study, a prognostic value of proliferative activity of the blastemal, i.e., blastemal MIB-1 expression was found for clinical progression and tumor-specific survival.…”

Section: Discussionmentioning

confidence: 99%

MIB-1 (KI-67) Proliferation Index and Cyclin-Dependent Kinase Inhibitor p27Kip1 Protein Expression in Nephroblastoma

Ghanem

Kwast

Sudaryo

et al. 2004

Clinical Cancer Research

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Purpose: A number of studies have indicated that the tumor proliferation marker MIB-1 and cell cycle inhibitor p27Kip1 expression are of prognostic importance in a variety of cancers. The present study was performed to evaluate the prognostic value of these molecules in Wilms' tumors.Experimental Design: MIB-1 and p27 Kip1 expressions were investigated by the means of immunohistochemical analysis of 62 Wilms' tumor. Patients were preoperatively treated by chemotherapeutic agents and had a mean follow-up of 5.7 years.Results: MIB-1 and p27 Kip1 were expressed in normal kidney tissues and in the three main components of Wilms' tumor, i.e., the blastemal, epithelial, and stromal cells. In Wilms' tumors, the percentage of MIB-1-positive cells in the blastema ranged between 0 and 42% (mean, 9.4%) and in the epithelial component between 0 and 53% (mean, 19.9%), with a significant difference (P < 0.01). The percentage of blastemal p27Kip1 -positive cells ranged between 3 and 85% (mean, 55.1%) and for the epithelial component between 1 and 87% (mean, 59%). There was a significant inverse relationship between blastemal MIB-1 and p27Kip1 expression in Wilms' tumor. Univariate analysis showed that blastemal MIB-1 and p27Kip1 expression were indicative for clinical progression and tumor-specific survival. In a multivariate analysis, blastemal MIB-1 and p27Kip1 protein expression proved to be an independent prognostic for clinical progression besides stage. Conclusions:It was concluded that both MIB-1-based proliferative activity and p27Kip1 protein expression in the blastema have prognostic impact in Wilms' tumor.

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Which proliferation markers for routine immunohistology? A comparison of five antibodies.

Abstract: For routine use, MIB1 or polyclonal Ki67 are the best proliferation markers in conventional histological preparations. The other markers tested cannot be recommended.

Cited by 105 publications

References 38 publications

Low-Grade Vulvar and Vaginal Intraepithelial Neoplasia: Correlation of Histologic Features with Human Papillomavirus DNA Detection and MIB-1 Immunostaining

Low-Grade Vulvar and Vaginal Intraepithelial Neoplasia: Correlation of Histologic Features with Human Papillomavirus DNA Detection and MIB-1 Immunostaining

Comparison of Ki-67 equivalent antibodies

MIB-1 (KI-67) Proliferation Index and Cyclin-Dependent Kinase Inhibitor p27Kip1 Protein Expression in Nephroblastoma

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