“Which Factors Affect Pregnancy Until Calving and Pregnancy Loss in Buffalo Recipients of in vitro Produced Embryos?”

Saliba, Wilson Pardini; Gimenes, Lindsay Unno; Drumond, Roberti; Bayão, Henrique Xavier Salgado; Palo, R. Di; Gasparrini, B.; Rubessa, M.; Baruselli, Pietro Sampaio; Sales, J. N. S.; Bastianetto, Eduardo; Leite, Rômulo Cerqueira; Alvim, M. T. T.

doi:10.3389/fvets.2020.577775

Cited by 6 publications

(11 citation statements)

References 36 publications

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“…Consequently, the recipient animal was probably the major driver behind the higher pregnancy rates achieved during the autumn and winter (18/56 = 32.1%), compared to the spring and summer (15/62 = 24.2%). This is consistent with previous studies in adult animals showing similar changes in pregnancy rate according to season [ 52 , 53 ]. Our findings further emphasize the role photoperiod has on seasonality, as our results are similar to other studies across the northern hemisphere [ 54 ], despite the fact that Canada’s climate involves much milder temperatures during spring and summer compared to other regions of the world (southern Italy for example).…”

Section: Discussionsupporting

confidence: 94%

Factors Affecting the Efficiency of In Vitro Embryo Production in Prepubertal Mediterranean Water Buffalo

Currin

Baldassarre

Macedo

et al. 2022

Animals

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Embryos from prepubertal water buffalo can be produced using laparoscopic ovum pickup (LOPU) and in vitro embryo production (IVEP). However, to date, it is unclear what factors and environmental conditions can affect LOPU-IVEP efficiency in prepubertal animals, especially buffalo. In this study, we explored the impact of season, age and individual variation among female donor animals, as well as the effect of the sire used for in vitro fertilization. Donor animals between 2 and 6 months of age were stimulated using gonadotropins prior to LOPU, which was performed at two-week intervals. Following in vitro maturation and fertilization, the resulting embryos were then cultured to the blastocyst stage until they were either vitrified or transferred into recipient animals. The number of follicles available for aspiration and embryo development rates was stable throughout the year. As animals became older, there was a slight trend for fewer COCs recovered from LOPU and better embryo development. There was a large individual variation in both ovarian response and the developmental competence of oocytes among donors. The bull used for fertilization also had a significant impact on embryo development. Upon embryo transfer, pregnancy rates were not affected by the number of embryos transferred per recipient. The best pregnancy rates were achieved when transferring blastocysts, compared to compact morula or hatched blastocysts. Finally, vitrification had no effect on pregnancy rate compared to fresh embryos.

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Section: Discussionsupporting

confidence: 94%

Factors Affecting the Efficiency of In Vitro Embryo Production in Prepubertal Mediterranean Water Buffalo

Currin

Baldassarre

Macedo

et al. 2022

Animals

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“…In buffaloes, the oocytes aspiration rates from slaughterhouse ovaries or the OPU (Di Francesco et al 2012), in vitro oocyte maturation (IVM) and blastocyst rates were decreased during the nonbreeding season (Sadeesh et al 2016). Also, the pregnancy losses were higher in the non-breeding season during artificial insemination (Qayyum et al 2018) or after the transfer of in vitro produced embryos in buffaloes (Saliba et al 2020). These results might be due to the increased daylight length with higher environmental temperature leading to hyperprolactinemia, the decreased secretion of gonadotropins and steroids.…”

Section: Seasons (Breeding or Non-breeding)mentioning

confidence: 99%

“…Many factors are responsible for the lower pregnancy or calving rates after the transfer of in vitro produced embryos. In bubaline, during an in vitro embryo production and transfer programme, chromosomal aberrations (Yoshizawa et al 2010), insufficient progesterone concentration (Saliba et al 2020) and improper contact of trophectoderm to the endometrium have been reported. In addition, errors in the elongation and attachment of the conceptus, germinal disc diameter, yolk sac development, binucleated cell numbers and foetal growth trajectory alteration have been reported in bovines (Ealy et al 2019).…”

Section: Advancements and Future Challengesmentioning

confidence: 99%

“…In this regard, Saliba et al (2020) observed that synchronisation protocols and recipient corpus luteum diameter influence the pregnancy and parturition rates after an embryo transfer in buffaloes. They found that Ovsynch (ovarian synchronisation) was superior to progesterone combined with estradiol and an ECG-based protocol.…”

Section: Advancements and Future Challengesmentioning

confidence: 99%

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Factors affecting the in vitro embryo production in buffalo (Bubalus bubalis): A review

Kumar¹,

MS²,

Silva³

et al. 2023

Vet. Med.

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Under natural and well-managed conditions, the buffalo has good reproductive and productive indices. However, in vitro embryo production (IVEP) has been used commercially to maximise the number of elite animals. In this species, several factors (donor management, in vitro culture medium, semen, in vitro conditions, embryo transfer) still affect the IVEP results. In addition, the cost of this technique is very high for this purpose. Therefore, more studies, as well as adequate plans, are needed to achieve this objective efficiently. In this review, we discussed the current commercial status, influencing factors (in vivo and in vitro), and the progress and future challenges of IVEP in buffalo. A total of 81 references were used from 1979 to 2022. The relevant data or literature were searched using the following databases: Google, ResearchGate, Science Alert, Science Direct and PubMed, using the following keywords: buffalo oocytes/COCs, buffalo embryos, pregnancy and calving or live birth rate after embryo transfer. The best maturation, cleavage and blastocyst rates in the in vitro production of buffalo embryos were 95.8, 75.2 and 33.4%, respectively. The pregnancy and live birth rates ranged from 22.2% to 43.5% and from 15.3% to 36.5%, respectively, after the transfer of fresh embryos produced in vitro to the recipients. This review will help to contextualise IVEP in buffaloes, as well as create an adequate plan for implementing IVEP in buffaloes.

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“…Live-born offspring from different sources of IVP embryos: fresh, frozen or vitrified (Baruselli et al, 2020) revealed that pregnancy and birth rates are low compared to cattle (10.5% vs. 26.9% pregnancy and 10.0% vs. 25.0% live births) (Marin et al, 2019). The effect of oocyte quality on blastocyst production and pregnancy rates, synchronisation protocol, progesterone levels and reproductive season has been recognised (Saliba et al, 2020), showing that for bubaline species there are many factors to study in order to offer this technology to producers. This aim of this study was to provide evidence from the literature and contribute to the improvement of buffalo embryo production, especially in Colombia.…”

Section: Introductionmentioning

confidence: 99%

In vitro production of buffalo embryos in Colombia – results of the addition of growth factors and antioxidants to defined culture medium

et al. 2022

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To increase the success rate of the in vitro production (IVP) system of embryos in buffalo, it is necessary to improve parameters. This field requires more research since its results are low compared to cattle. In this study carried out in Colombia, a modification of the methodology to produce buffalo IVP embryos is described, and 24 non-lactating females, aged 4 to 11 years old were included. Follicular wave was synchronised by ablation of the follicles within the ovary, seven days later all follicles were aspirated to obtain oocytes for maturation, fertilisation and embryo production. For all culture media used, animal protein was avoided. Culture media were supplemented with growth factors and other molecules associated with embryonic development. Special care was taken in adding supplements to protect the zygotes against the harmful effect of oxygen free radicals in the culture medium. Two aspiration series were performed 15 days apart. The average number of oocytes obtained by aspiration was 11.33, the number of blastocysts was 3.05, and the obtained blastocyst production rate (36.2%) was higher than literature reports for this species; we hypothesise that this increase is associated with the supplements added. It was possible to observe a female that produced 50 oocytes. Subsequent research is needed to evaluate the effect of the medium on embryo quality and pregnancy rates. A cost analysis of the new proposed medium is required, since all supplements must be imported and handled with a cold chain of up to -80oC, making it more expensive and restricting widespread use. Finally, it is shown that with three embryos per aspiration, at least one pregnancy can be successful after the aspiration, which is one of the objectives of the IVP system.

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“Which Factors Affect Pregnancy Until Calving and Pregnancy Loss in Buffalo Recipients of in vitro Produced Embryos?”

Cited by 6 publications

References 36 publications

Factors Affecting the Efficiency of In Vitro Embryo Production in Prepubertal Mediterranean Water Buffalo

Factors Affecting the Efficiency of In Vitro Embryo Production in Prepubertal Mediterranean Water Buffalo

Factors affecting the in vitro embryo production in buffalo (Bubalus bubalis): A review

In vitro production of buffalo embryos in Colombia – results of the addition of growth factors and antioxidants to defined culture medium

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