Which elements are involved in reversible and irreversible cartilage degradation in osteoarthritis?

Bay‐Jensen, Anne‐Christine; Hoegh-Madsen, Suzi; Dam, Erik B.; Henriksen, Kim; Søndergaard, B.C.; Pastoureau, Philippe; Qvist, Per; Karsdal, Morten A.

doi:10.1007/s00296-009-1183-1

Cited by 112 publications

(90 citation statements)

References 40 publications

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“…The chondrocyte is the unique resident cell of articular cartilage and thus solely responsible for ECM composition and regulation. Chondrocyte metabolism is influenced by its micro-environment, and in return influences ECM composition, organization and ultimately the mechanical resilience of cartilage [1][2][3]. As such, chondrocytes play a key role in ECM remodelling in physiological and pathological conditions [4].…”

Section: Introductionmentioning

confidence: 99%

Chondrocyte secretome: a source of novel insights and exploratory biomarkers of osteoarthritis

Sanchez

Bay‐Jensen

Pap

et al. 2017

Osteoarthritis and Cartilage

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The extracellular matrix (ECM) of articular cartilage is comprised of complex networks of proteins and glycoproteins, all of which are expressed by its resident cell, the chondrocyte. Cartilage is a unique tissue given its complexity and ability to resist repeated load and deformation. The mechanisms by which articular cartilage maintains its integrity throughout our lifetime is not fully understood, however there are numerous regulatory pathways known to govern ECM turnover in response to mechanical stimuli. To further our understanding of this field, we envision that proteomic analysis of the secretome will provide information on how the chondrocyte remodels the surrounding ECM in response to load, in addition to providing information on the metabolic state of the cell. In this review, we attempt to summarize the recent mass spectrometry-based proteomic discoveries in healthy and diseased cartilage and chondrocytes, to facilitate the discovery of novel biomarkers linked to degenerative pathologies, such as osteoarthritis (OA).

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Section: Introductionmentioning

confidence: 99%

Chondrocyte secretome: a source of novel insights and exploratory biomarkers of osteoarthritis

Sanchez

Bay‐Jensen

Pap

et al. 2017

Osteoarthritis and Cartilage

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“…With preventive strategies available to modify joint biomechanics by surgical interventions or to improve tissue resilience by pharmaceutical agents, the very earliest evidence of degeneration must be detected and adequately addressed. Early detection of degeneration is crucial as the pathology may be reversible at this point [5].…”

Section: Introductionmentioning

confidence: 99%

Optical coherence tomography-based parameterization and quantification of articular cartilage surface integrity

Brill

Riedel

Rath

et al. 2015

Biomed. Opt. Express

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Loss of articular cartilage surface integrity is considered the earliest sign of osteoarthritis; however, its reliable detection has not been established by clinical routine diagnostics. This study comprehensively assesses a set of 11 algorithm-based 2-D Optical Coherence Tomography roughness parameters and investigates their clinical impact. Histology and manual irregularity quantification of 105 human cartilage samples with variable degeneration served as reference. The majority of parameters revealed a close-to-linear correlation with the entire spectrum of degeneration. Surface integrity should therefore be assessed by a combination of parameters to improve current diagnostic accuracy in the determination of cartilage degeneration.

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“…Matrix metalloproteinases (MMPs) play an essential role in the degradation of ECM proteins such as collagens and proteoglycans. 7,8 Serum MMP-3, a key enzyme in ECM remodeling, is strongly elevated in AS patients. 9,10 Increased turnover of several different MMP-derived collagen species has been accepted as a main feature of AS.…”

Section: Introductionmentioning

confidence: 99%

Histopathological changes in supraspinous ligaments, ligamentum flava and paraspinal muscle tissues of patients with ankylosing spondylitis

Zhang

et al. 2014

Int J of Rheum Dis

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Objective: To examine the histopathological changes in spinal tissues of ankylosing spondylitis (AS) patients.Methods: Tissue samples from 10 AS patients and 10 control subjects were obtained. Hematoxylin and eosin, picrosirius, Masson and van Gieson stainings were utilized to determine the pathological changes in tissues. Ultrastructural alterations were examined by electronic microscopy. Proteoglycan levels were assessed by enzyme-linked immunosorbent assays (ELISA). Matrix metalloproteinase-3 (MMP-3), transforming growth factor-b1 (TGF-b1) and tumor necrosis factor-a (TNF-a) levels were evaluated by immunohistochemistry.Results: Our results demonstrate that the density of collagen fibrils was reduced in the supraspinous ligaments of AS tissue and fibrils were loosely and irregularly organized as compared to a regular distribution of collagen fibrils in controls. In ligamentum flava from AS patients, activated fibroblasts with enlarged nuclei were detected, while the number of elastic fibers was greatly decreased. Paraspinal muscle tissues of AS patients exhibited increased collagen fibril accumulation and atrophy. Significantly decreased proteoglycan and elevated MMP-3 levels were found in supraspinous ligament samples from AS patients (P < 0.01). Additionally, the levels of TGF-b1 in ligamentum flava and paraspinal muscle tissues of AS patients were increased (P < 0.01). The expression of TNF-a was also upregulated in the ligamentum flavum (P < 0.01), with no significant difference in the paraspinal muscle between control and AS patients (P > 0.05).Conclusions: Our findings reveal histopathological changes that occur in certain spinal tissues of AS patients and suggest that increased levels of MMP-3 and TGF-b1 may contribute to the pathogenesis of AS.

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Which elements are involved in reversible and irreversible cartilage degradation in osteoarthritis?

Cited by 112 publications

References 40 publications

Chondrocyte secretome: a source of novel insights and exploratory biomarkers of osteoarthritis

Chondrocyte secretome: a source of novel insights and exploratory biomarkers of osteoarthritis

Optical coherence tomography-based parameterization and quantification of articular cartilage surface integrity

Histopathological changes in supraspinous ligaments, ligamentum flava and paraspinal muscle tissues of patients with ankylosing spondylitis

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