Whey protein hydrolysate enhances the exercise-induced heat shock protein (HSP70) response in rats

Moura, Carolina Soares; Lollo, Pablo Christiano Barboza; Morato, Priscila Neder; Carneiro, Everardo M.; Amaya-Farfán, Jaime

doi:10.1016/j.foodchem.2012.09.070

Cited by 38 publications

(40 citation statements)

References 29 publications

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“…Protein carbonyls, biomarkers of oxidative stress were lower in the group that consumed whey protein hydrolysate. From the result, it was inferred that the consumption of whey protein hydrolysate enhances HSP70 expression (De Moura et al 2013). An extension of this study indicated that the whey protein hydrolysate is caoable of augmenting cell survival factors such as HSP90 and vascular endothelial growth factor (VEGF) (Moura et al 2014).…”

Section: Antioxidant Anti-inflammation and Hepato-protectionmentioning

confidence: 99%

Emerging trends in nutraceutical applications of whey protein and its derivatives

Patel

2015

J Food Sci Technol

125

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The looming food insecurity demands the utilization of nutrient-rich residues from food industries as valueadded products. Whey, a dairy industry waste has been characterized to be excellent nourishment with an array of bioactive components. Whey protein comprises 20 % of total milk protein and it is rich in branched and essential amino acids, functional peptides, antioxidants and immunoglobulins. It confers benefits against a wide range of metabolic diseases such as cardiovascular complications, hypertension, obesity, diabetes, cancer and phenylketonuria. The protein has been validated to boost recovery from resistance exercise-injuries, stimulate gut physiology and protect skin against detrimental radiations. Apart from health invigoration, whey protein has proved its suitability as fat replacer and emulsifier. Further, its edible and antimicrobial packaging potential renders its highly desirable in food as well as pharmaceutical sectors. Considering the enormous nutraceutical worth of whey protein, this review emphasizes on its established and emerging biological roles. Present and future scopes in food processing and dietary supplement formulation are discussed. Associated hurdles are identified and how technical advancement might augment its applications are explored. This review is expected to provide valuable insight on whey protein-fortified functional foods, associated technical hurdles and scopes of improvement.

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Section: Antioxidant Anti-inflammation and Hepato-protectionmentioning

confidence: 99%

Emerging trends in nutraceutical applications of whey protein and its derivatives

Patel

2015

J Food Sci Technol

125

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“…Supplemental lipoic acid, for instance, was seen to cause an increase in the expression of HSP90, which was associated with a rise in protection to the skeletal muscle of horses exercised on a treadmill [18]. In rodents, treadmill exercise has been successfully used to induce HSP response and alter in homeostasis [1,2,19]. …”

Section: Discussionmentioning

confidence: 99%

“…De Moura et al [1] showed that WPH stimulates glutamine synthetase expression, an enzyme capable of harnessing the nitrogen donated by BCAAs to generate glutamine. Therefore, we determined the concentration of free glutamine in plasma and the results demonstrated that lle-Leu peptide that influenced various immune markers also induced greater free glutamine plasma concentration.…”

Section: Discussionmentioning

confidence: 99%

“…Whey proteins (WPs) are known to be functional for promoting the improvement of several biological responses that include cytoprotective effects mediated by the enhancement of heat shock protein (HSP) expression, promotion of glycogen content, stimulation of muscle protein synthesis, modulation of the immune response and improvement of satiety [1–6]. In particular, the HSPs are a natural endogenous defense system capable of promoting cell resistance and tolerance against a variety of stressors, protecting and repairing damage.…”

Section: Introductionmentioning

confidence: 99%

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Bioactivity of food peptides: biological response of rats to bovine milk whey peptides following acute exercise

Moura

Lollo

Morato

et al. 2017

Food & Nutrition Research

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Background: Several physiologically beneficial effects of consuming a whey protein hydrolysate (WPH) have been attributed to the greater availability of bioactive peptides. Aims: The aim was to investigate the effect of four branched-chain amino acid- (BCAA-)containing dipeptides, present in WPH, on immune modulation, stimulation of HSP expression, muscle protein synthesis, glycogen content, satiety signals and the impact of these peptides on the plasma free amino acid profiles. Methods: The animals were divided in groups: control (rest, without gavage), vehicle (water), L-isoleucyl-L-leucine (lle-Leu), L-leucyl-L-isoleucine (Leu-lle), L-valyl-Lleucine (Val-Leu), L-leucyl-L-valine (Leu-Val) and WPH. All animals were submitted to acute exercise, except for control. Results: lle-Leu stimulated immune response, hepatic and muscle glycogen and HSP60 expression, whereas Leu-Val enhanced HSP90 expression. All dipeptides reduced glucagon-like peptide-1 and glucose-dependent insulinotropic polypeptide, no changes were observed on leptin. All peptides inhibited NF-kB expression. The plasma amino acid time-course showed peptide-specific and isomer-specific metabolic features, including increases of the BCAAs. Conclusion: The data indicate that lle-Leu was effective to attenuate immune-suppression exercise-induced, promoted glycogen content and stimulated anti-stress effect (HSP). Furthermore, Leu-Val increased HSP90, p-4EBP1, p-mTOR and p-AMPK expression. The data suggest the involvement of these peptides in various beneficial functions of WPH consumption.

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“…In any case, a combination of resistance exercise plus nutritional interventions has been raised as promising in treating sarcopenia [26,148]. This is reinforced by the fact that whey protein hydrolysate (WPH), a notable source of glutamine dipeptide, was found to enhance exercise-induced HSP70 response in rats [326].…”

Section: Physical Exercise Glutamine and Hs Response In Agingmentioning

confidence: 99%

Physiological regulation of the heat shock response by glutamine: implications for chronic low-grade inflammatory diseases in age-related conditions

Leite

Cruzat

Krause

et al. 2016

Nutrire

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Aging is an intricate process modulated by different molecular and cellular events, such as genome instability, epigenetic and transcriptional changes, molecular damage, cell death and senescence, inflammation, and metabolic dysfunction. Particularly, protein quality control (chaperone systems) tends to be negatively affected by aging, thus leading to cellular senescence in metabolic tissues and, as a consequence, to the increasing dissemination of inflammation throughout the body. The heat shock (HS) response and its associated expression of the 70 kDa family of heat shock proteins (HSP70), which are anti-inflammatory molecular chaperones, are found to be markedly decreased during muscle inactivity and aging, while evidence supports the loss of HSP70 as a key mechanism which may drive muscle atrophy, contractile dysfunction, and reduced regenerative capacity. In addition, abnormal stress response is linked with higher incidence of neurodegenerative diseases as well as low-grade inflammatory diseases that are associated with physical inactivity and obesity. Therefore, strategies to increase or, at least, to maintain the levels of HSP70, and its accompanying HS response to stress, are key to reduce biological cell dysfunctions that occur in aging. In this sense, physical exercise is of note as it is the most powerful inducer of the HS response, comparable only to heat stress and fever-like conditions. On the other hand, the amino acid L-glutamine, whose production within the skeletal muscle and liberation into the blood stream is dependent on muscle activity, is a potentializer of HSP70 expression and HS response, particularly via its entering in hexosamine biosynthetic pathway (HBP). Herein, we discuss the collaborative role of glutamine (and its donors/precursors) and physical exercise (mostly responsible for glutamine release into the circulation) as potential tools to increase HSP70 expression and the HS response in the elderly.

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Whey protein hydrolysate enhances the exercise-induced heat shock protein (HSP70) response in rats

Cited by 38 publications

References 29 publications

Emerging trends in nutraceutical applications of whey protein and its derivatives

Emerging trends in nutraceutical applications of whey protein and its derivatives

Bioactivity of food peptides: biological response of rats to bovine milk whey peptides following acute exercise

Physiological regulation of the heat shock response by glutamine: implications for chronic low-grade inflammatory diseases in age-related conditions

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