Where macrolide resistance is prevalent

Li, Yarong; Liu, Xiaoguai; Bei, Zhang; He, Qiushui; Wang, Zengguo

doi:10.1111/apm.12357

Cited by 7 publications

(7 citation statements)

References 10 publications

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“…It was recently reported that B . pertussis is susceptible to ceftazidime, cefepime and a carbapenem, namely, imipenem [ 35 ], which might be used as alternatives for pertussis treatment. Although B .…”

Section: Discussionmentioning

confidence: 99%

Variation in Bordetella pertussis Susceptibility to Erythromycin and Virulence-Related Genotype Changes in China (1970-2014)

Yang

Yao

et al. 2015

PLoS ONE

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ObjectivesTo investigate changes in virulence-related genotypes and in the antimicrobial susceptibility of Bordetella pertussis isolates collected from the 1970s to 2014 in the northern part of China.MethodsA total of 124 B. pertussis isolates from three periods, the 1970s, 2000–2008, and May 2013–Sept 2014, were typed by multilocus sequence typing (MLST) and tested for antimicrobial susceptibility and virulence-related genes. A fragment of the 23S rRNA gene from each of the 99 isolates from 2013–2014 was amplified and sequenced.ResultsAll isolates from 2000–2008 and 2013–2014 were identified as ST2, whereas isolates from the 1970s were ST1. PtxA2/ptxC1/ptxP1/prn1/fim2-1/fim3-1/tcfA2, which was the same as the vaccine strain, was the only type in the 1970s. During the 2000s and 2013–2014, the virulence type ptxA1/ptxC1/ptxP1/prn1/fim2-1/fim3-1/tcfA2 was dominant, with frequencies of 68.4% and 91.9%, respectively. Nine ptxP3 strains, which were more virulent, were detected after 2000. All 124 isolates were susceptible to levofloxacin, sulphamethoxazole/trimethoprim and tetracycline. The isolates from the 1970s and 2000–2008 were susceptible to all tested macrolides, whereas 91.9% of the 2013–2014 isolates were highly resistant (minimal inhibitory concentration, MIC >256 μg/ml). No ptxP3 strain was resistant to macrolides. All erythromycin-resistant strains except for one had the A2047G mutation in the 23S rRNA gene.ConclusionsMacrolide resistance of the B. pertussis population has been a serious problem in the northern part of China. Because most of the epidemic clone of the pathogen expresses the same antigen profiles as the vaccine strain, except ptxA, improvements in immunization strategies may prevent the spread of infection and drug resistance.

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Section: Discussionmentioning

confidence: 99%

Variation in Bordetella pertussis Susceptibility to Erythromycin and Virulence-Related Genotype Changes in China (1970-2014)

Yang

Yao

et al. 2015

PLoS ONE

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“…Azithromycin is another commonly used macrolide and more active than erythromycin against B. pertussis. In our previous study, we analyzed the MICs of azithromycin against 14 erythromycinresistant and 2 erythromycin-sensitive strains (9). Although the MICs of azithromycin against the 16 tested strains were generally lower than those of erythromycin, all strains except the 2 that were erythromycin sensitive were also resistant to azithromycin, suggesting that the A2047G mutation in domain V of 23S rRNA of B. pertussis might be implicated in azithromycin resistance, at least among the clinical isolates analyzed in this study.…”

Section: Discussionmentioning

confidence: 87%

“…Fourteen erythromycin-resistant B. pertussis strains with the confirmed mutation A2047G and 2 susceptible strains without the mutation were used for this study (9). The 16 clinical strains were isolated between 2012 and 2013 during a prospective study conducted in Xi'an, China (7,9).…”

Section: Methodsmentioning

confidence: 99%

“…They were isolated throughout the study period and not from local small outbreaks. Erythromycin MICs ranged from 0.023 to Ͼ256 g/ml, and all 14 erythromycin-resistant strains had MICs of Ͼ256 g/ml (9). During the prospective study, NP swab samples were taken from 313 patients with suspected pertussis infection and tested for culture and PCRs (targeting IS481 and ptx-Pr).…”

Section: Methodsmentioning

confidence: 99%

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Direct Detection of Erythromycin-Resistant Bordetella pertussis in Clinical Specimens by PCR

et al. 2015

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Resistance of Bordetella pertussis to erythromycin has been increasingly reported. We developed an allele-specific PCR method for rapid detection of erythromycin-resistant B. pertussis directly from nasopharyngeal (NP) swab samples submitted for diagnostic PCR. Based on the proven association of erythromycin resistance with the A2047G mutation in the 23S rRNA of B. pertussis, four primers, two of which were designed to be specific for either the wild-type or the mutant allele, were used in two different versions of the allele-specific PCR assay. The methods were verified with results obtained by PCR-based sequencing of 16 recent B. pertussis isolates and 100 NP swab samples submitted for diagnostic PCR. The detection limits of the two PCR assays ranged from 10 to 100 fg per reaction for both erythromycin-susceptible and -resistant B. pertussis. Two amplified fragments of each PCR, of 286 and 112 bp, respectively, were obtained from a mutant allele of the isolates and/or NP swab samples containing B. pertussis DNAs. For the wild-type allele, only a 286-bp fragment was visible when the allele-specific PCR assay 1 was performed. No amplification was found when a number of non-Bordetella bacterial pathogens and NP swab samples that did not contain the DNAs of B. pertussis were examined. This assay can serve as an alternative for PCR-based sequencing, especially for local laboratories in resource-poor countries. P ertussis has resurged in many countries. Macrolides, especially erythromycin, are considered the first-choice antibiotics for treatment of pertussis and postexposure prophylaxis (1). The first erythromycin-resistant Bordetella pertussis was discovered in 1994 (2). Although resistant strains are still rare, they have been reported in several countries, including China (3-7), where macrolide-resistant B. pertussis has become prevalent (7).B. pertussis is a fastidious bacterium. The culture positivity rate is low in immunized populations. The PCR method has been developed and used for diagnosis of pertussis in many laboratories all over the world. However, it is possible that resistant B. pertussis strains are missed due to failure of the culture and when culture is not performed (8).Several studies have proven the association between macrolide resistance and the A2047G mutation in the 23S rRNA of B. pertussis (3-7). Based on the molecular mechanism identified, methods have been developed for detection of the point mutation in 23S rRNA of B. pertussis in order to study the susceptibility to macrolides when B. pertussis isolates are available (3, 5). In a previous study, we developed a PCR-based sequencing method for identification of the A2047G mutation in cultured B. pertussis isolates and clinical nasopharyngeal (NP) specimens (7).Although the sequencing is widely used, it is not available or accessible everywhere because it takes longer to obtain the results in many of the local laboratories, especially in resource-poor countries. In this study, we aimed to develop a simple allele-specific PCR method for di...

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“…Изоляты S. aureus, устойчивые к аминогликозидам, продуцируют три типа аминогликозид-модифицирующих ферментов (AME) ( (31,32). Изучение устойчивости S. aureus к макролидам показывает, что erm, кодирующие рибосомальные метилазы, -наиболее часто встречающиеся гены, определяющие устойчивость к эритромицину (33,34). В настоящей работе впервые описана суточная динамика активности штаммов S. aureus из молока высокопродуктивных коров в среде с высокими концентрациями антибактериальных препаратов.…”

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PHENOTYPIC RESISTANCE TO ANTIBIOTICS OF Staphylococcus aureus STRAINS ISOLATED FROM COW MILK

Артемьева¹

2019

S-h. biol.

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Из-за постоянного использования антимикробных препаратов в последние десятилетия резко обострилась проблема появления мультирезистентных штаммов. Инфекции, вызванные Staphylococcus aureus, наиболее распространены у высокопродуктивных животных молочного направления. Целью нашей работы было оценить фенотипическую устойчивость штаммов Staphylococcus aureus, выделенных из молока коров, к основным антибактериальным препаратам, используемым для лечения различных форм мастита на территории Российской Федерации, а также определить их минимальные ингибирующие концентрации (MIC, MIC 50 и MIC 90). Пробы молока отбирали в хозяйствах Центрального региона России в период с января по декабрь 2018 года от коров черно-пестрой голштинизированной породы. Всего было исследовано 314 образцов молока и выделено 447 потенциальных изолятов стафилококков. Все изоляты оценивали общепринятыми фенотипическими методами. Только 103 удовлетворяли всем критериям идентификации и использовались для последующего анализа. Тестирование чувствительности выделенных штаммов проводили диско-диффузным методом (ДДМ) к следующим антибиотикам: пенициллину (Pen, 10 ЕД), оксациллину (OX, 10 мкг), гентамицину (GN, 10 мкг), эритромицину (ER, 15 мкг), линкомицину (LN, 15 мкг), рифампицину (RF, 5 мкг), ципрофлоксацину (CP, 5 мкг), ванкомицину (VA, 30 мкг), фузидину (FZ, 10 мкг) (ЗАО «Научно-исследовательский центр фармакотерапии», Россия) на среде Mueller Hinton Agar («HiMedia Laboratories Pvt. Ltd.», Индия). Минимальные ингибирующие концентрации (MIC, MIC 50 , MIC 90) и LD 50 определяли методом 2кратных (Log 2) серийных разведений (от 64 до 0,125 мг/л) антибиотиков (пенициллин, эритромицин, гентамицин, ципрофлоксацин) в среде TSB с инокулюмом 5½10 5 КОЕ/мл. Инкубирование осуществляли при 37 С в течение 20 ч на качалке (220 об/мин). Рост культур в среде TSB с антибиотиками оценивали при пересеве на среду Baird Parker Agar («HiMedia Laboratories Pvt. Ltd.», Индия). Результаты интерпретировали по рекомендации The European Committee on Antimicrobial Susceptibility Testing (EUCAST). Наивысшая степень устойчивости наблюдалась к эритромицину (82,5 %) и фузидину (75,7 %). Из 103 штаммов 7 были чувствительны ко всем исследуемым антибиотикам, 96 изолятов оказались устойчивы по крайней мере к одному из них. При оценке множественной устойчивости (MAR) 65 (63,1 %) штаммов оказались резистентны к четырем и более антибиотикам. Преобладающими фенотипами для выделенных изолятов были ER + LN + FZ и ER + CP (58,3 % и 47,6 % изолятов). Из 47 изолятов, устойчивых к пенициллину по ДДМ, 31 штамм продемонстрировал рост при концентрациях пенициллина  64 мг/л, при этом в диапазоне  1,0 мг/л рост наблюдался у 33,0 % штаммов. Из 85 изолятов, устойчивых к эритромицину, 15 проявляли рост при MIC  64 мг/л, в диапазон MIC  0,5 мг/л попали только 7 штаммов из 18 чувствительных по ДДМ. Из 103 исследуемых штаммов 29 (28,2 %) были устойчивы к гентамицину по ДДМ и 72 (60,2 %) проявляли рост при MIC  0,5 мг/л, а 5 штаммов-при MIC  64,0 мг/л. Из 49 устойчивых по результатам ДДМ штаммов только у...

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Where macrolide resistance is prevalent

Cited by 7 publications

References 10 publications

Variation in Bordetella pertussis Susceptibility to Erythromycin and Virulence-Related Genotype Changes in China (1970-2014)

Variation in Bordetella pertussis Susceptibility to Erythromycin and Virulence-Related Genotype Changes in China (1970-2014)

Direct Detection of Erythromycin-Resistant Bordetella pertussis in Clinical Specimens by PCR

PHENOTYPIC RESISTANCE TO ANTIBIOTICS OF Staphylococcus aureus STRAINS ISOLATED FROM COW MILK

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