Where do IgA plasma cells in the gut come from?

Golby, S J C; Spencer, Jo

doi:10.1136/gut.51.2.150

Cited by 11 publications

(8 citation statements)

References 15 publications

(9 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Locally, subepithelial plasma cells produce immunoglobulins which are secreted into the oviductal lumen (Kutteh et al 1990). This indicates that plasma cells may have a significant role in the early defence against the invasion of micro-organisms at the mucosal surfaces of the infundibulum as suggested being the case for intestinal mucosa (Kroese et al 1994;Golby and Spencer 2002).…”

Section: Discussionmentioning

confidence: 99%

The Sow Endosalpinx at Different Stages of the Oestrous Cycle and at Anoestrus: Studies on Morphological Changes and Infiltration by Cells of the Immune System

Jiwakanon¹,

Persson

Kaeoket³

et al. 2005

Reprod Domestic Animals

View full text Add to dashboard Cite

The oviduct, together with its secretion, forms the environment in which gamete transport, sperm maturation, fertilization as well as early embryo transport and development occur. Morphological aspects, as well as immune cell distribution, have been described only to a limited extent regarding the pig oviduct. To better understand the reproductive physiology, more information is needed regarding morphology and infiltration of immune cells at different stages of the oestrous cycle.The aim of this study was to investigate the morphological changes and the distribution of leukocytes in the sow endosalpinx throughout the oestrous cycle and at anoestrus. Nineteen crossbred sows (Swedish Landrace × Swedish Yorkshire) at late dioestrus (3), prooestrus (3), oestrus (3), early dioestrus (3), dioestrus (3) and anoestrus (4) were used. Oviductal samples from three different parts (isthmus, ampulla and infundibulum), taken immediately after slaughter, were immersion-fixed, embedded in plastic resin and stained with toluidine blue or stored in a freezer at -70°C until analysed by immunohistochemistry (prooestrus and anoestrus) with an avidin-biotin peroxidase method. Quantitative and qualitative examinations of oviductal epithelial and subepithelial connective tissue layers were performed by light microscopy.During all stages, a lower degree of morphological changes in the epithelium (pseudostratification, mitosis and secretory granules) was found in the isthmus compared with ampulla and infundibulum. In the ampulla and infundibulum, pseudostratification, mitotic activity and secretory granules were high at prooestrus/oestrus. Cytoplasmic protrusions of epithelial cells with some extruded nuclei were prominent in ampulla and infundibulum at all stages except for oestrus and early dioestrus.Lymphocytes as well as CD2 and CD3 positive cells were the predominant immune cells in the epithelial layer. The numbers of lymphocytes and CD3 positive cells did not differ among segments and stages. Numbers of CD2 positive cells did not differ between prooestrus and anoestrus while the numbers were significantly higher in the infundibulum than in ampulla and isthmus. Neutrophils were only occasionally found and mainly in the infundibulum.In the subepithelial connective tissue layer, the two most commonly observed immune cell types were lymphocytes and plasma cells. The numbers of lymphocytes as well as CD2 and CD3 positive cells was lower in isthmus than in the other segments (P ≤ 0.001). Higher numbers of plasma cells (P ≤ 0.001) were found in infundibulum than in ampula and isthmus. The numbers of lymphocytes and plasma cells were not significantly different between stages of the oestrous cycle. However, the number of neutrophils differed and were highest at prooestrus in ampulla and infundibulum. The numbers of CD2, CD3 and CD79 positive cells did not differ between prooestrus and anoestrus whereas for CD14 and SWC3 positive cells, the numbers were higher at prooestrus (P ≤ 0.05) than at anoestrus.In the oviduct, the morphology differ...

show abstract

Section: Discussionmentioning

confidence: 99%

The Sow Endosalpinx at Different Stages of the Oestrous Cycle and at Anoestrus: Studies on Morphological Changes and Infiltration by Cells of the Immune System

Jiwakanon¹,

Persson

Kaeoket³

et al. 2005

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…The gastrointestinal barrier has two components: the intrinsic barrier, which is composed of a continually renewing sheet of epithelial cells with their tight junctions (2)(3)(4)(5)(6)(7) and the extrinsic barrier, which consists of mucus, bicarbonate, prostaglandins, growth factors, trefoil proteins and immunoglobulins (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Mucosal Damage and Recovery in the Gastrointestinal Tract of Rats by a Penetration Enhancer

et al. 2007

View full text Add to dashboard Cite

show abstract

“…B1-derived lymphocytes are capable of T cell-independent activation and this pathway contributes to roughly 50% of the total intestinal sIgA release. 62,63 …”

Section: What Is the Gi Immune System?mentioning

confidence: 99%