When Simple Meets Complex: Pollen and the -Omics

Fíla, Jan; Drábková, Lenka Záveská; Gibalová, Antónia; Honys, David

doi:10.1007/978-3-319-56645-0_10

Cited by 8 publications

(6 citation statements)

References 201 publications

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“…Moreover, the data obtained by the proteomic analysis are functional to deepening the physiology of pollen tube growth regulation especially considering that recent gene expression studies are focused on RNAseq or massive analysis through on gene chip transcriptomics. As reported by Fíla et al [24], for systems with high level of translational regulation, such as the male gametophyte the transcriptional analysis must be validated by proteomic studies. As a matter of fact, translation and mRNA storage are active mechanisms during male gametophyte development and it is desirable to complement transcriptomics with proteomic data to get more realistic insights.…”

Section: Discussionmentioning

confidence: 99%

Protein Analysis of Pollen Tubes after the Treatments of Membrane Trafficking Inhibitors Gains Insights on Molecular Mechanism Underlying Pollen Tube Polar Growth

et al. 2021

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Pollen tube elongation is characterized by a highly-polarized tip growth process dependent on an efficient vesicular transport system and largely mobilized by actin cytoskeleton. Pollen tubes are an ideal model system to study exocytosis, endocytosis, membrane recycling, and signaling network coordinating cellular processes, structural organization and vesicular trafficking activities required for tip growth. Proteomic analysis was applied to identify Nicotiana tabacum Differentially Abundant Proteins (DAPs) after in vitro pollen tube treatment with membrane trafficking inhibitors Brefeldin A, Ikarugamycin and Wortmannin. Among roughly 360 proteins separated in two-dimensional gel electrophoresis, a total of 40 spots visibly changing between treated and control samples were identified by MALDI-TOF MS and LC–ESI–MS/MS analysis. The identified proteins were classified according to biological processes, and most proteins were related to pollen tube energy metabolism, including ammino acid synthesis and lipid metabolism, structural features of pollen tube growth as well modification and actin cytoskeleton organization, stress response, and protein degradation. In-depth analysis of proteins corresponding to energy-related pathways revealed the male gametophyte to be a reliable model of energy reservoir and dynamics.

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Section: Discussionmentioning

confidence: 99%

Protein Analysis of Pollen Tubes after the Treatments of Membrane Trafficking Inhibitors Gains Insights on Molecular Mechanism Underlying Pollen Tube Polar Growth

et al. 2021

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“…The expression data for A . thaliana were obtained from eFP Browser [ 30 ] and from other publicly available resources as described previously [ 49 , 50 , 51 ]. Briefly, the transcriptome datasets were downloaded from the NASCArray microarray database through the AffyWatch service [ 52 ] and normalized using freely available dChip 1.3 software ( http://www.dchip.org ) to the median probe intensity level; model-based gene-expression values were based on the Perfect Match-only model [ 53 , 54 ].…”

Section: Methodsmentioning

confidence: 99%

Evolutionary history of callose synthases in terrestrial plants with emphasis on proteins involved in male gametophyte development

Drábková

Honys

2017

PLoS ONE

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Callose is a plant-specific polysaccharide (β-1,3-glucan) playing an important role in angiosperms in many developmental processes and responses to biotic and abiotic stresses. Callose is synthesised at the plasma membrane of plant cells by callose synthase (CalS) and, among others, represents the main polysaccharide in the callose wall surrounding the tetrads of developing microspores and in the growing pollen tube wall. CalS proteins involvement in spore development is a plesiomorphic feature of terrestrial plants, but very little is known about their evolutionary origin and relationships amongst the members of this protein family. We performed thorough comparative analyses of callose synthase family proteins from major plant lineages to determine their evolutionary history across the plant kingdom. A total of 1211 candidate CalS sequences were identified and compared amongst diverse taxonomic groups of plants, from bryophytes to angiosperms. Phylogenetic analyses identified six main clades of CalS proteins and suggested duplications during the evolution of specialised functions. Twelve family members had previously been identified in Arabidopsis thaliana. We focused on five CalS subfamilies directly linked to pollen function and found that proteins expressed in pollen evolved twice. CalS9/10 and CalS11/12 formed well-defined clades, whereas pollen-specific CalS5 was found within subfamilies that mostly did not express in mature pollen vegetative cell, although were found in sperm cells. Expression of five out of seven mature pollen-expressed CalS genes was affected by mutations in bzip transcription factors. Only three subfamilies, CalS5, CalS10, and CalS11, however, formed monophyletic, mostly conserved clades. The pairs CalS9/CalS10, CalS11/CalS12 and CalS3 may have diverged after angiosperms diversified from lycophytes and bryophytes. Our analysis of fully sequenced plant proteins identified new evolutionary lineages of callose synthase subfamilies and has established a basis for understanding their functional evolution in terrestrial plants.

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“…Molecular and genetic studies of male germline development documented in earlier reviews are dominated by those of angiosperms (Berger & Twell, 2011;Russell & Jones, 2015;Twell, 2011). Other recent reviews focus on omics (Fíla et al, 2017;Rutley & Twell, 2015), chromatin remodelling (Borg & Berger, 2015), post-transcriptional processes (Guan et al, 2013;Hafidh et al, 2016;Michard et al, 2017), or wider aspects of reproductive cell lineages (Schmidt et al, 2015). Here we focus on regulatory aspects of male gametogenesis including comparative studies and advances in pre-angiosperm models.…”

Section: Male Germline Development In Angiospermsmentioning

confidence: 99%

The evolution and patterning of male gametophyte development

Hackenberg

Twell

2019

Current Topics in Developmental Biology

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1. The evolutionary history of the male gametophyte 1.1. The origins of spermatogenesis in green plants 1.2. The sporophyte was key for terrestrial plant evolution 1.3. Heterospory and the origin of microgametophytes 1.4. Adaptations of seed plant microgametophytes 2. Male germline development in angiosperms 2.1. Asymmetric division and cell fate determination 2.2. Cell cycle control and male gametophyte patterning 2.3. Transcriptional control of male gametophyte patterning 2.4. Chromatin remodelling in male gametophyte development 2.5. Communication between non-germ and germ cells 3. Molecular evolution of the male germline 3.1. Motile plant and animal sperm share common features 3.2. Gene expression in male germ cells 3.3. The evolution of transcription factors regulating male gametogenesis 4. Perspective 5. Acknowledgements 2 6. References Abstract: The reproductive adaptations of land plants have played a key role in their terrestrial colonization and radiation. These encompass mechanisms used for the production, dispersal and union of gametes to support sexual reproduction. The production of small motile male gametes and larger immotile female gametes (oogamy) in specialized multicellular gametangia evolved in the charophyte algae, the closest extant relatives of land plants.Reliance on water and motile male gametes for sexual reproduction was retained by bryophytes and basal vascular plants, but was overcome in seed plants by the dispersal of pollen and the guided delivery of non-motile sperm to the female gametes. Here we discuss the evolutionary history of male gametogenesis in streptophytes (green plants) and the underlying developmental biology, including recent advances in bryophyte and angiosperm models. We conclude with a perspective on research trends that promise to deliver a deeper understanding of the evolutionary and developmental mechanisms of male gametogenesis in plants.

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When Simple Meets Complex: Pollen and the -Omics

Cited by 8 publications

References 201 publications

Protein Analysis of Pollen Tubes after the Treatments of Membrane Trafficking Inhibitors Gains Insights on Molecular Mechanism Underlying Pollen Tube Polar Growth

Protein Analysis of Pollen Tubes after the Treatments of Membrane Trafficking Inhibitors Gains Insights on Molecular Mechanism Underlying Pollen Tube Polar Growth

Evolutionary history of callose synthases in terrestrial plants with emphasis on proteins involved in male gametophyte development

The evolution and patterning of male gametophyte development

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