When local anesthesia becomes universal: Pronounced systemic effects of subcutaneous lidocaine in bullfrogs ( Lithobates catesbeianus )

Williams, Catherine J.; Alstrup, Aage K.O.; Bertelsen, Mads F.; Jensen, Hans Henrik; Leite, Cléo A. C.; Wang, Tobias

doi:10.1016/j.cbpa.2017.03.019

Cited by 14 publications

(2 citation statements)

References 37 publications

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“…The cardiac rate (in situ f H ) of both experimental groups ( Bd − and Bd + ) from both species was determined visually (for 3 min) and expressed as beats per minute (bpm). Although this procedure does not exclude all the effects of adrenergic tonus, it avoids the side effects of anesthetics used for the insertion of ECG electrodes ( Wassersug, Paul & Feder, 1981 ; Williams et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

Novel findings on the impact of chytridiomycosis on the cardiac function of anurans: sensitive vs. tolerant species

Salla

Rizzi-Possignolo

Oliveira

et al. 2018

PeerJ

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BackgroundUnderstanding of the physiological effects of chytridiomycosis is crucial to worldwide amphibian conservation. Therefore, we analyzed the cardiac function of two anuran species (Xenopus laevis and Physalaemus albonotatus) with different susceptibilities to infection by the causative agent of chytridiomycosis, Batrachochytrium dendrobatidis (hereafter Bd).MethodsWe analyzed the in situ heart rate (fH - bpm), relative ventricular mass (RVM -%), and Ca2+ handling in heart of Bd infected animals compared to uninfected controls of both study species.ResultsBd infection resulted in a 78% decrease in contraction force values in P. albonotatus when compared to the less susceptible X. laevis. This negative effect was even more evident (82%) for the cardiac pumping capacity. The time to reach peak tension was 125% longer in P. albonotatus than in X. laevis, and cardiac relaxation was 57% longer.DiscussionThese results indicate a delay in the cardiac cycle of P. albonotatus on a beat-to-beat basis, which was corroborated by the bradycardia observed in situ. In summary, Bd-sensitive species present impaired cardiac function, which could be a factor in mortality risk. The more pronounced effects of Bd in P. albonotatus may not only result from electrolyte imbalance, as previously reported, but also could be an effect of toxins produced by Bd. For X. laevis, the ability to promote cardiac adjustments seems to be an important homeostatic feature that allows greater tolerance to chytridiomycosis. This study provides new physiological mechanisms underlying the tolerance or susceptibility of amphibian species to chytridiomycosis, which determine their adaptability to survive in the affected environments.

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Section: Methodsmentioning

confidence: 99%

Novel findings on the impact of chytridiomycosis on the cardiac function of anurans: sensitive vs. tolerant species

Salla

Rizzi-Possignolo

Oliveira

et al. 2018

PeerJ

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“…Moreover, sustaining administration of local anesthetic is challenging to carry out, which leads to weak compliance [12,13]. In addition, lidocaine could trigger numerous adverse effects, such as vasodilation, serious arrhythmias, and depleted peripheral resistance [14][15][16]. Therefore, versatile drug delivery systems have been engineered to facilitate prolongation of lidocaine release locally at the treatment site and lessen toxicity.…”

Section: Introductionmentioning

confidence: 99%

Graphene Oxide-Reinforced Alginate Hydrogel for Controlled Release of Local Anesthetics: Synthesis, Characterization, and Release Studies

Luu

Nguyen

et al. 2022

Gels

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In pain relief, lidocaine has gained more attention as a local anesthetic. However, there are several side effects that limit the use of local anesthetics. Therefore, it is hypothesized that a hydrogel system with facile design can be used for prolonged release of lidocaine. In this study, we developed a formulation comprises of sodium alginate (SA) and graphene oxide (GO) to prolong the release of lidocaine. The gelation was induced by physically crosslinking the alginate with Ca2+ ions. The formation of blank SA and GO-reinforced SA hydrogels was investigated with different concentration of Ca2+ ions. The controlled release of lidocaine hydrochloride (LH) on both hydrogel systems was studied in PBS solution. The GO-reinforced SA hydrogels exhibited more sustained release than SA hydrogels without GO. In vitro biocompatibility test in L929 fibroblast cells confirmed the non-toxic property of hydrogels. Furthermore, to prove the in-situ gelation and biodegradability of hydrogels the hydrogels were injected on mice model and confirmed the stable gel formation. The hydrogels implanted onto the subcutaneous tissue of hydrogels retained over one week. These results indicate that LH-loaded GO-reinforced SA hydrogel can be a potential biomaterial for controlled release of local anesthetics.

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Anesthesia and analgesia in amphibians

Williams

Bertelsen

2023

Anesthesia and Analgesia in Laboratory Animals

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When local anesthesia becomes universal: Pronounced systemic effects of subcutaneous lidocaine in bullfrogs ( Lithobates catesbeianus )

Cited by 14 publications

References 37 publications

Novel findings on the impact of chytridiomycosis on the cardiac function of anurans: sensitive vs. tolerant species

Novel findings on the impact of chytridiomycosis on the cardiac function of anurans: sensitive vs. tolerant species

Graphene Oxide-Reinforced Alginate Hydrogel for Controlled Release of Local Anesthetics: Synthesis, Characterization, and Release Studies

Anesthesia and analgesia in amphibians

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