When green and red mycology meet: Impressions from an interdisciplinary forum on virulence mechanisms of phyto- and human-pathogenic fungi

Yu, Yidong; Hube, Bernhard; Kämper, Jörg; Meyer, Vera; Krappmann, Sven

doi:10.1080/21505594.2017.1356502

Cited by 3 publications

(3 citation statements)

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“…Plant and human pathogenic fungi share common pathogenicity factors that are important for understanding common aspects of infection and virulence to develop novel antifungal strategies and fungicides to control pathogen growth (Fones et al, 2017;Yu et al, 2017). The transcription factor AfSomA plays important roles in adhesion, conidia formation and virulence in the human pathogenic fungus A. fumigatus (Lin et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Verticillium dahliae transcription factors Som1 and Vta3 control microsclerotia formation and sequential steps of plant root penetration and colonisation to induce disease

et al. 2018

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Summary Verticillium dahliae nuclear transcription factors Som1 and Vta3 can rescue adhesion in a FLO8‐deficient Saccharomyces cerevisiae strain. Som1 and Vta3 induce the expression of the yeast FLO1 and FLO11 genes encoding adhesins. Som1 and Vta3 are sequentially required for root penetration and colonisation of the plant host by V. dahliae. The SOM1 and VTA3 genes were deleted and their functions in fungus‐induced plant pathogenesis were studied using genetic, cell biology, proteomic and plant pathogenicity experiments. Som1 supports fungal adhesion and root penetration and is required earlier than Vta3 in the colonisation of plant root surfaces and tomato plant infection. Som1 controls septa positioning and the size of vacuoles, and subsequently hyphal development including aerial hyphae formation and normal hyphal branching. Som1 and Vta3 control conidiation, microsclerotia formation, and antagonise in oxidative stress responses. The molecular function of Som1 is conserved between the plant pathogen V. dahliae and the opportunistic human pathogen Aspergillus fumigatus. Som1 controls genes for initial steps of plant root penetration, adhesion, oxidative stress response and VTA3 expression to allow subsequent root colonisation. Both Som1 and Vta3 regulate developmental genetic networks required for conidiation, microsclerotia formation and pathogenicity of V. dahliae.

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Section: Discussionmentioning

confidence: 99%

Verticillium dahliae transcription factors Som1 and Vta3 control microsclerotia formation and sequential steps of plant root penetration and colonisation to induce disease

et al. 2018

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“…Filamentous fungi are ubiquitously found in nature as they are capable of adapting to diverse environments. Some species such as Aspergillus nidulans and Neurospora crassa serve as model organisms to study biological processes, others such as Aspergillus niger , Aspergillus oryzae , Penicillium chrysogenum , and Trichoderma reesei are used extensively as workhorses in biotechnology, and numerous others are pathogens for plants, animals and/or humans or employed for biocontrol [ 1 , 2 , 3 , 4 , 5 , 6 ]. Growth studies, for example to compare wild-type and mutant strains or to elucidate the response to different nutrients, are mainly carried out on solid media.…”

Section: Introductionmentioning

confidence: 99%

Online Biomass Monitoring Enables Characterization of the Growth Pattern of Aspergillus fumigatus in Liquid Shake Conditions

Bauer

Abt

Yap

et al. 2022

JoF

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Numerous filamentous fungal species are extensively studied due to their role as model organisms, workhorses in biotechnology, or as pathogens for plants, animals, and humans. Growth studies are mainly carried out on solid media. However, studies concerning gene expression, biochemistry, or metabolism are carried out usually in liquid shake conditions, which do not correspond to the growth pattern on solid media. The reason for this practice is the problem of on-line growth monitoring of filamentous fungal species, which usually form pellets in liquid shake cultures. Here, we compared the time-consuming and tedious process of dry-weight determination of the mold Aspergillus fumigatus with online monitoring of biomass in liquid shake culture by the parallelizable CGQ (“cell growth quantifier”), which implements dynamic biomass determination by backscattered light measurement. The results revealed a strong correlation of CGQ-mediated growth monitoring and classical biomass measurement of A. fumigatus grown over a time course. Moreover, CGQ-mediated growth monitoring displayed the difference in growth of A. fumigatus in response to the limitation of iron or nitrogen as well as the growth defects of previously reported mutant strains (ΔhapX, ΔsrbA). Furthermore, the frequently used wild-type strain Af293 showed largely decreased and delayed growth in liquid shake cultures compared to other strains (AfS77, A1160p+, AfS35). Taken together, the CGQ allows for robust, automated biomass monitoring of A. fumigatus during liquid shake conditions, which largely facilitates the characterization of the growth pattern of filamentous fungal species.

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“…This escalation is closely associated with the expanding population of susceptible individuals, particularly patients who are immunocompromised, from organ transplant recipients to those undergoing autoimmune disease treatments, and includes those afflicted by immunosuppressive conditions, such as HIV infection and AIDS. Notably, the mortality toll of fungal infections is nearly as high the global burden of malaria and tuberculosis [3].…”

Section: Introductionmentioning

confidence: 99%

Polypeptides Targeting Paracoccidioides brasiliensis Drk1

Marcos,

de Oliveira,

Assato

et al. 2023

JoF

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Considering the toxicity of conventional therapeutic approaches and the importance of precise mechanistic targets, it is important to explore signaling pathways implicated in fungal pathobiology. Moreover, treatment of paracoccidioidomycosis, a systemic mycosis caused by a dimorphic fungus, requires prolonged therapeutic regimens. Among the numerous factors underpinning the establishment of Paracoccidioides spp. infection, the capacity to transition from the mycelial to the yeast form is of pivotal importance. The Drk1 protein of Paracoccidioides brasiliensis likely plays a decisive role in this morphological shift and subsequent virulence. We identified peptides with affinity for the PbDrk1 protein using the phage-display method and assessed the effects of these peptides on P. brasiliensis. The peptides were found to inhibit the phase transition of P. brasiliensis. Furthermore, a substantial proportion of these peptides prevented adhesion to pneumocytes. Although these peptides may not possess inherent antifungal properties, they can augment the effects of certain antifungal agents. Notably, the cell wall architecture of P. brasiliensis appears to be modulated by peptide intervention, resulting in a reduced abundance of glycosylated proteins and lipids. These peptides were also evaluated for their efficacy in a Galleria mellonella model and shown to contribute to enhanced larval survival rates. The role of PbDrk1, which is notably absent in mammals, should be further investigated to improve the understanding of its functional role in P. brasiliensis, which may be helpful for designing novel therapeutic modalities.

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When green and red mycology meet: Impressions from an interdisciplinary forum on virulence mechanisms of phyto- and human-pathogenic fungi

Abstract: (2017) When green and red mycology meet: Impressions from an interdisciplinary forum on virulence mechanisms of phyto-and human-pathogenic fungi,

Cited by 3 publications

References 80 publications

Verticillium dahliae transcription factors Som1 and Vta3 control microsclerotia formation and sequential steps of plant root penetration and colonisation to induce disease

Verticillium dahliae transcription factors Som1 and Vta3 control microsclerotia formation and sequential steps of plant root penetration and colonisation to induce disease

Online Biomass Monitoring Enables Characterization of the Growth Pattern of Aspergillus fumigatus in Liquid Shake Conditions

Polypeptides Targeting Paracoccidioides brasiliensis Drk1

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