When does determination occur in Drosophila embryos?

Simcox, Amanda; Sang, James H.

doi:10.1016/0012-1606(83)90078-7

Cited by 103 publications

(32 citation statements)

References 11 publications

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“…1a). The discs are formed and become determined (wingness, legness) during embryogenesis (Simcox & Sang, 1983). They maintain their determination throughout larval development.…”

Section: Drosophila Imaginal Discs: a Model System To Study Cell Plasmentioning

confidence: 99%

Transdetermination: Drosophila imaginal disc cells exhibit stem cell-like potency

McClure

Schubiger

2007

The International Journal of Biochemistry & Cell Biology

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Drosophila imaginal discs, the primordia of the adult fly appendages, are an excellent system for studying developmental plasticity. Cells in the imaginal discs are determined for their disc-specific fate (wingness, legness) during embryogenesis. Disc cells maintain their determination during larval development, a time of extensive growth and proliferation. Only when prompted to regenerate do disc cells exhibit lability in their determined identity. Regeneration in the disc is mediated by a localized region of cell division, known as the regeneration blastema. Most regenerating disc cells strictly adhere to their disc-specific identity; some cells however, switch fate in a phenomenon known as transdetermination. Similar regeneration and transdetermination events can be induced in situ by misexpression of the signaling molecule wingless. Recent studies indicate that the plasticity of disc cells during regeneration is associated with high morphogen activity and the reorganization of chromatin structure. Here we provide both a historical perspective of imaginal disc transdetermination, as well as discuss recent findings on how imaginal disc cells acquire developmental plasticity and multipotency. We also highlight how an understanding of imaginal disc transdetermination can enhance an understanding of developmental potency exhibited by stem cells.

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“…1a). The discs are formed and become determined (wingness, legness) during embryogenesis (Simcox & Sang, 1983). They maintain their determination throughout larval development.…”

Section: Drosophila Imaginal Discs: a Model System To Study Cell Plasmentioning

confidence: 99%

Transdetermination: Drosophila imaginal disc cells exhibit stem cell-like potency

McClure

Schubiger

2007

The International Journal of Biochemistry & Cell Biology

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“…By the time nuclei have cellularized (the cellular blastoderm), individual cells must have received instruction as to their relative positions in the embryo (Simcox and Sang, 1983). Though largely speculative, it is often proposed that a chemical gradient across a spatial domain is produced and imparts this positional information to cells (Lawrence, 1973;Meinhardt, 1977;Kauffman et al, 1978;Russell, 1985).…”

Section: What Governs Engrailed Expressionmentioning

confidence: 99%

“…Cellularization of each of these nuclei produces the cellular blastoderm. Although the embryo shows no morphologically evident pattern at this stage, a molecular prepattern is somehow encoded within individual cells because the approximate position to which their descendents will contribute is already specified (Wieschaus and Gehring, 1976;Szabad et al, 1979;Simcox and Sang, 1983). During a 7 hr period of morphogenetic movements of gastrulation this prepattern is interpreted and becomes visibly manifest as the basic segmental divisions.…”

Section: Introductionmentioning

confidence: 99%

Development of embryonic pattern in D. melanogaster as revealed by accumulation of the nuclear engrailed protein

DiNardo

1985

Cell

396

193

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Summaryengrailed is required to establish and maintain developmental compartments within each segment of the fly. To understand the role of the engrailed protein in this process, we have raised antibodies against engrailed and have visualized an engrailed protein in embryos by indirect immunofluorescence. The protein accumulates in the nucleus, supporting the notion that engrailed is a regulatory factor. The first pattern of expression is in alternating segments followed by expression in every segment, suggesting that engrailed may be responding to pair-rule segmentation gene products. Overall, engrailed protein levels peak in areas undergoing morphogenesis. Finally, the complex final form of the head and terminalia derive from earlier simple subdivision of these areas into developmental fields by engrailed.

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“…At this time, the first signs of diversification of the unstructured chromatin into domains with distinct functional organisation are observable: the first heterochromatin foci can be visualised (James et al, 1989;Vlassova et al, 1991). This structuring of the epigenome coincides with cell fate determination (Simcox and Sang, 1983).…”

Section: Introductionmentioning

confidence: 99%

Developmental role for ACF1-containing nucleosome remodellers in chromatin organisation

et al. 2010

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SUMMARYThe nucleosome remodelling complexes CHRAC and ACF of Drosophila are thought to play global roles in chromatin assembly and nucleosome dynamics. Disruption of the gene encoding the common ACF1 subunit compromises fly viability. Survivors show defects in chromatin assembly and chromatin-mediated gene repression at all developmental stages. We now show that ACF1 expression is under strict developmental control. The expression is strongly diminished during embryonic development and persists at high levels only in undifferentiated cells, including the germ cell precursors and larval neuroblasts. Constitutive expression of ACF1 is lethal. Cell-specific ectopic expression perturbs chromatin organisation and nuclear programmes. By monitoring heterochromatin formation during development, we have found that ACF1-containing factors are involved in the initial establishment of diversified chromatin structures, such as heterochromatin. Altering the levels of ACF1 leads to global and variegated deviations from normal chromatin organisation with pleiotropic defects.

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When does determination occur in Drosophila embryos?

Cited by 103 publications

References 11 publications

Transdetermination: Drosophila imaginal disc cells exhibit stem cell-like potency

Transdetermination: Drosophila imaginal disc cells exhibit stem cell-like potency

Development of embryonic pattern in D. melanogaster as revealed by accumulation of the nuclear engrailed protein

Developmental role for ACF1-containing nucleosome remodellers in chromatin organisation

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