2021
DOI: 10.1111/pbi.13573
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Wheat with greatly reduced accumulation of free asparagine in the grain, produced by CRISPR/Cas9 editing of asparagine synthetase gene TaASN2

Abstract: Free asparagine is the precursor for acrylamide, which forms during the baking, toasting and high-temperature processing of foods made from wheat. In this study, CRISPR/Cas9 was used to knock out the asparagine synthetase gene, TaASN2, of wheat (Triticum aestivum) cv. Cadenza. A 4-gRNA polycistronic gene was introduced into wheat embryos by particle bombardment and plants were regenerated. T1 plants derived from 11 of 14 T0 plants were shown to carry edits. Most edits were deletions (up to 173 base pairs), but… Show more

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Cited by 60 publications
(47 citation statements)
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References 40 publications
(65 reference statements)
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“…Cadenza using the CRISPR/Cas9 system. The study provided strong evidence that very low-asparagine commercial wheat varieties can be produced, allowing for the development of lower-acrylamide bread, cereals, biscuits, and other wheat-based foods [70].…”
Section: Application Of Crispr/cas9 System In Wheat Genome Editingmentioning
confidence: 88%
See 1 more Smart Citation
“…Cadenza using the CRISPR/Cas9 system. The study provided strong evidence that very low-asparagine commercial wheat varieties can be produced, allowing for the development of lower-acrylamide bread, cereals, biscuits, and other wheat-based foods [70].…”
Section: Application Of Crispr/cas9 System In Wheat Genome Editingmentioning
confidence: 88%
“…It was also demonstrated that having only one wild-type copy of each of the three TaNP1 genes was enough to maintain male fertility [69]. In a recent study, in order to reduce the expression of asparagine synthetase in grain without affecting its expression in any other part of the plant, Raffan et al (2021) targeted the TaASN2 gene in T. aestivum cv. Cadenza using the CRISPR/Cas9 system.…”
Section: Application Of Crispr/cas9 System In Wheat Genome Editingmentioning
confidence: 99%
“…Acrylamide in food is a processing contaminant that forms from free asparagine and potentially increases the risk of developing cancer for humans. In wheat, Raffan et al (2021) knocked out the asparagine synthetase gene TaASN2 using four guide RNAs targeting all three homologues of TaASN2. Compared with the wild type, the concentration of free asparagine in seeds of the plants with all six TaASN2 alleles edited was significantly decreased, up to 90%.…”
Section: Acrylamidementioning
confidence: 99%
“…To take a more recent example, in May 2021, the UK's Rothamsted Research applied for a permit to field test wheat that has been gene-edited to contain less asparagine, an amino acid that becomes the carcinogen acrylamide when cooked at high temperatures (Rothamsted 2021;Raffan et al 2021). The research institute explained that the "low acrylamide" wheat was modified using CRISPR to reduce the production of this chemical, which may cause cancer.…”
Section: Root-cause Problems Do Gene Editors and Agroecologists Have A Shared Analysis Of The Underlying Problem(s)?mentioning
confidence: 99%