Wheat TaPUB1 modulates plant drought stress resistance by improving antioxidant capability

Zhang, Guangqiang; Zhang, Meng; Zhao, Zhongxian; Ren, Yuanqing; Li, Qinxue; Wang, Wei

doi:10.1038/s41598-017-08181-w

Cited by 57 publications

(43 citation statements)

References 54 publications

(60 reference statements)

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“…Our results indicate that AtPUB46 is a positive regulator of the response to drought as OE plants display improved tolerance and knock-down mutants are hypersensitive [11]. This is similar to OE of rice OsPUB15 [28], or wheat TaPUB1 [29] in transgenic rice or Nicotiana benthamiana, respectively, which also increased drought tolerance. This is in marked contrast to the majority of PUB genes that have been studied where overexpression resulted in hypersensitivity to drought [30][31][32][33][34][35].…”

Section: 1supporting

confidence: 64%

Overexpression of Arabidopsis ubiquitin ligase AtPUB46 enhances tolerance to drought and oxidative stress

Adler

Mishra

Maymon

et al. 2018

Preprint

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The U-Box E3 ubiquitin ligase, AtPUB46, functions in the drought response: T-DNA insertion mutants of this single paralogous gene are hypersensitive to water- and oxidative stress (Adler et al. BMC Plant Biology 17:8, 2017). Here we analyze the phenotype of AtPUB46 overexpressing (OE) plants. AtPUB46-OE show increased tolerance to water stress and have smaller leaf blades and reduced stomatal pore area and stomatal index compared with wild type (WT). Despite this, the rate of water loss from detached rosettes is similar in AtPUB46-OE and WT plants. Germination of AtPUB46-OE seeds was less sensitive to salt than WT whereas seedling greening was more sensitive. We observed a complex response to oxidative stress applied by different agents: AtPUB46-OE plants were hypersensitive to H2O2 but hyposensitive to methyl viologen. AtPUB46-GFP fusion protein is cytoplasmic, however, in response to H2O2 a considerable proportion translocates to the nucleus. We conclude that the differential stress phenotype of the AtPUB46-OE does not result from its smaller leaf size but from a change in the activity of a stress pathway(s) regulated by a degradation substrate of the AtPUB46 E3 and also from a reduction in stomatal pore size and index.Accession NumbersSequence data for this article can be found in: The Arabidopsis Information Resource database (http://www.arabidopsis.org) under accession numbers At5G18320 (PUB46).

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Section: 1supporting

confidence: 64%

Overexpression of Arabidopsis ubiquitin ligase AtPUB46 enhances tolerance to drought and oxidative stress

Adler

Mishra

Maymon

et al. 2018

Preprint

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“…The CAT, SOD, ascorbate peroxidase (APX), POD and contents of soluble sugar and proline were detected according to previous reports (Zhang et al 2017a).…”

Section: Methodsmentioning

confidence: 99%

“…In our previous study, a wheat gene TaPUB1 was identified. By heterologous overexpression of TaPUB1 in the dicotyledonous model plant Nicotiana benthamiana , we found this gene improved plant salt and drought stress tolerance (Zhang et al 2017a, 2017b). However, it remained unclear whether TaPUB1 could regulate the tolerance of wheat under salt stress.…”

Section: Introductionmentioning

confidence: 95%

The involvement of wheat U‐box E3 ubiquitin ligase TaPUB1 in salt stress tolerance

Wang

et al. 2019

JIPB

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U-box E3 ubiquitin ligases play important roles in the ubiquitin/26S proteasome machinery and in abiotic stress responses. TaPUB1-overexpressing wheat (Triticum aestivum L.) were generated to evaluate its function in salt tolerance. These plants had more salt stress tolerance during seedling and flowering stages, whereas the TaPUB1-RNA interference (RNAi)-mediated knock-down transgenic wheat showed more salt stress sensitivity than the wild type (WT). TaPUB1 overexpression upregulated the expression of genes related to ion channels and increased the net root Na + efflux, but decreased the net K + efflux and H + influx, thereby maintaining a low cytosolic Na + /K + ratio, compared with the WT. However, RNAi-mediated knock-down plants showed the opposite response to salt stress. TaPUB1 could induce the expression of some genes that improved the antioxidant capacity of plants under salt stress. TaPUB1 also interacted with TaMP (Triticum aestivum α-mannosidase protein), a regulator playing an important role in salt response in yeast and in plants. Thus, low cytosolic Na + / K + ratios and better antioxidant enzyme activities could be maintained in wheat with overexpression of TaPUB1 under salt stress. Therefore, we conclude that the U-box E3 ubiquitin ligase TaPUB1 positively regulates salt stress tolerance in wheat.

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“…To cope with this, plants have evolved enzymatic mechanisms for ROS scavenging . Hence, following physio‐morphological trait examinations, other indicators, such as redox status, cell membrane stability indexes, and ROS scavenging enzyme activities are normally measured to estimate the stress status …”

Section: Introductionmentioning

confidence: 99%

Differential adaptation strategies to different levels of soil water deficit in two upland and lowland genotypes of rice: a physiological and metabolic approach

et al. 2019

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BACKGROUND: Upland genotypes of rice are less sensitive to soil water deficit (SWD), making them suitable candidates for revealing the strategies underlying plant tolerance. The physiological factors, the biochemical traits needed to withstand oxidative stress, and the metabolite fluctuations of an upland genotype (Azucena) and an intolerant lowland genotype (IR64) genotype were measured under two levels of SWD (withholding water for 7-or 14 days) to identify SWD-responsive strategies associated with tolerance. (up to 1.79-log2FC) in the Azucena leaves may be indicative of their transport to the roots, supplying the carbon source needed for root elongation. Under a 14-day treatment, proline and aspartate family members accumulated to the highest levels in Azucena, whereas an increase in the levels of aromatic amino acids with key roles in the biosynthesis of secondary metabolites was detected in IR64. RESULTS: After withholding water for 7 days, no significant changes in physiological and biochemical traits of Azucena were observed, whereas in IR64, significant decreases in physiological factors were recorded along with increases in oxidative-stress indicators. However, the root length of Azucena increased significantly, showing a clear stress avoidance strategy. Under a prolonged treatment (14 days), IR64 entered an oxidative-damage stage, whereas Azucena exhibited a highly efficient antioxidant system. Our metabolite analysis also revealed two different enriched pathways. After a 7-day SWD, the sugar levels were decreased in the leaves of Azucena but increased in IR64. The reduction in the sugar levelsCONCLUSION: The adaptation strategies identified in two types of rice genotypes in confronting SWD may assist researchers in finding the proper indicators for screening more tolerant genotypes.

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Wheat TaPUB1 modulates plant drought stress resistance by improving antioxidant capability

Cited by 57 publications

References 54 publications

Overexpression of Arabidopsis ubiquitin ligase AtPUB46 enhances tolerance to drought and oxidative stress

Overexpression of Arabidopsis ubiquitin ligase AtPUB46 enhances tolerance to drought and oxidative stress

The involvement of wheat U‐box E3 ubiquitin ligase TaPUB1 in salt stress tolerance

Differential adaptation strategies to different levels of soil water deficit in two upland and lowland genotypes of rice: a physiological and metabolic approach

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