Wheat Storage Proteins in Transgenic Rice Endosperm

Oszvald, Mária; Balázs, G.; Pólya, Sára; Tömösközi, Sándor; Appels, R.; Békés, F.; Tamás, László

doi:10.1021/jf402035n

Cited by 11 publications

(6 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The expression of wheat HMW-GSs, 1Dx5_KK, and 1Dy10_JK, was observed in KK and JK transgenic lines, respectively. Oszvald et al [ 32 , 33 ] reported that the significant over-expression of 1Dx5 in rice seeds results in changing storage protein composition and increasing disulfide isomerase (PDI) levels, causing a positive effect on the functional properties of rice dough. Previous studies reported that the significant suppression of one SSP is compensated for by the increased accumulation of other SSPs, including PDI [ 1 , 40 , 41 ].…”

Section: Resultsmentioning

confidence: 99%

“…In addition, the over-expression of wheat 1Dx5 and/or 1Dy10 HMW-GSs improves the extensibility and elasticity of dough from wheat [ 26 , 27 , 28 ] and rye [ 29 ]. In efforts that were aimed at improving rice dough quality, preliminary attempts have been made to express wheat SSPs, including 1Ax1 [ 30 ], 1Bx7 [ 31 ], 1Dx5 [ 32 , 33 ], and 1Dy10 [ 34 ] in rice seeds, although to date, only 1Dx5 has been shown to confer a positive effect on rice dough properties. The objective of this study was to engineer wheat HMW-GS into rice seeds.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cellular Localization of Wheat High Molecular Weight Glutenin Subunits in Transgenic Rice Grain

Cho

Lee

et al. 2017

IJMS

View full text Add to dashboard Cite

Rice (Oryza sativa L.) is a primary global food cereal. However, when compared to wheat, rice has poor food processing qualities. Dough that is made from rice flour has low viscoelasticity because rice seed lacks storage proteins that are comparable to gluten protein from wheat. Thus, current research efforts aim to improve rice flour processing qualities through the transgenic expression of viscoelastic proteins in rice seeds. In this study, we characterized the transgenic expression of wheat glutenin subunits in rice seeds. The two genes 1Dx5_KK and 1Dy10_JK, which both encode wheat high-molecular-weight glutenin subunits that confer high dough elasticity, were cloned from Korean wheat cultivars KeumKang and JoKyung, respectively. These genes were inserted into binary vectors under the control of the rice endosperm-specific Glu-B1 promoter and were expressed in the high-amylose Korean rice cultivar Koami (Oryza sativa L.). Individual expression of both glutenin subunits was confirmed by SDS-PAGE and immunoblot analyses performed using T3 generation of transgenic rice seeds. The subcellular localization of 1Dx5_KK and 1Dy10_JK in the rice seed endosperm was confirmed by immunofluorescence analysis, indicating that the wheat glutenin subunits accumulate in protein body-II and novel protein body types in the rice seed. These results contribute to our understanding of engineered seed storage proteins in rice.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cellular Localization of Wheat High Molecular Weight Glutenin Subunits in Transgenic Rice Grain

Cho

Lee

et al. 2017

IJMS

View full text Add to dashboard Cite

show abstract

“…Proteomic analysis of the transgenic line (Oszvald et al , 2013) showed that the levels of some molecular chaperones were significantly higher compared with non-transformed, control rice lines. This suggests that although the recombinant HMW-GS represents <0·8 % of the total storage proteins, its expression may be enough to trigger the ER stress response and therefore affect protein sorting and PB formation.…”

Section: Discussionmentioning

confidence: 99%

The trafficking pathway of a wheat storage protein in transgenic rice endosperm

Oszvald¹,

Tamás²,

Shewry³

et al. 2014

Annals of Botany

View full text Add to dashboard Cite

Background and AimsThe trafficking of proteins in the endoplasmic reticulum (ER) of plant cells is a topic of considerable interest since this organelle serves as an entry point for proteins destined for other organelles, as well as for the ER itself. In the current work, transgenic rice was used to study the pattern and pathway of deposition of the wheat high molecular weight (HMW) glutenin sub-unit (GS) 1Dx5 within the rice endosperm using specific antibodies to determine whether it is deposited in the same or different protein bodies from the rice storage proteins, and whether it is located in the same or separate phases within these.MethodsThe protein distribution and the expression pattern of HMW sub-unit 1Dx5 in transgenic rice endosperm at different stages of development were determined using light and electron microscopy after labelling with antibodies.Key resultsThe use of HMW-GS-specific antibodies showed that sub-unit 1Dx5 was expressed mainly in the sub-aleurone cells of the endosperm and that it was deposited in both types of protein body present in the rice endosperm: derived from the ER and containing prolamins, and derived from the vacuole and containing glutelins. In addition, new types of protein bodies were also formed within the endosperm cells.ConclusionsThe results suggest that the HMW 1Dx5 protein could be trafficked by either the ER or vacuolar pathway, possibly depending on the stage of development, and that its accumulation in the rice endosperm could compromise the structural integrity of protein bodies and their segregation into two distinct populations in the mature endosperm.

show abstract

“…Studies on the GM modification of such subunits may also lead to the production of novel proteins encoded by altered versions of either the transforming or endogenous genes (Blechl and Vensel, 2013). A relevant similar study is that on transgenic rice seed expressing the wheat HMW subunit (Oszvald et al, 2013). Another aspect of this type of study that has importance in any future regulatory submission is the determination of potential changes in the allergenicity of the GM material (Lupi et al, 2013).…”

Section: Yield Traitsmentioning

confidence: 99%

Transgenic cereals: Current status and future prospects

Dunwell

2014

Journal of Cereal Science

View full text Add to dashboard Cite

This review summarises the history of transgenic (GM) cereals, principally maize, and then focuses on the scientific literature published in the last two years. It describes the production of GM cereals with modified traits, divided into input traits and output traits. The first category includes herbicide tolerance and insect resistance, and resistance to abiotic and biotic stresses; the second includes altered grains for starch, protein or nutrient quality, the use of cereals for the production of high value medical or other products, and the generation of plants with improved efficiency of biofuel production. Using data from field trial and patent databases the review considers the diversity of GM lines being tested for possible future development. It also summarises the dichotomy of response to GM products in various countries, describes the basis for the varied public acceptability of such products, and assesses the development of novel breeding techniques in the light of current GM regulatory procedures.

show abstract

Wheat Storage Proteins in Transgenic Rice Endosperm

Cited by 11 publications

References 50 publications

Cellular Localization of Wheat High Molecular Weight Glutenin Subunits in Transgenic Rice Grain

Cellular Localization of Wheat High Molecular Weight Glutenin Subunits in Transgenic Rice Grain

The trafficking pathway of a wheat storage protein in transgenic rice endosperm

Transgenic cereals: Current status and future prospects

Contact Info

Product

Resources

About