Wheat Storage Proteins: Glutenin Diversity in Sphaerococcum Mutant Forms of Tetraploid Wheats

Georgiev, S.; Dekova, T.

doi:10.1080/13102818.2005.10817274

Cited by 2 publications

(1 citation statement)

References 22 publications

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“…New insertions of BARE-1 / WIS 2-1A into glutenin and gamma-gliadin genes suggest that transposon dynamics affect the structure and probably the function of these loci. These important data support previous findings [25] showing the presence of polymorphism in relation to the glutenin and gliadin protein patterns within sphaerococcum mutant forms.…”

Section: Bare-1/wis 2-1a Markers Are Efficient For Mutant Diversity Asupporting

confidence: 82%

Retrotransposons and ethyl methanesulfonate-induced diversity in hexaploid wheat and Triticale

2010

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Abstract:The bulk of large plant genomes consists of retrotransposons. Retrotransposons are able to integrate into a multitude of loci in the genome, and can thereby generate insertional polymorphism between individuals, as well as mutations and new characteristics. Retrotransposons are largely quiescent during development, but become more active in response to biotic and abiotic stresses, which cause them to produce larger pools of transcripts. The present study assesses the structural dynamics and putative transcriptional activation of BARE-1 and WIS 2-1A retrotransposons in ethyl methanesulfonate (EMS)-induced hexaploid wheat and Triticale sphaerococcum mutants, via an RT-PCR approach that utilized the retrotransposon based markers SSAP, IRAP and REMAP. Our results demonstrate a polymorphic pattern distribution of BARE-1/WIS 2-1A members, and identify new insertions. The WIS 2-1A retrotransposon members have a modulated transcriptional profile, which strongly suggests that EMS treatment encourages their activation Retrotransposon-based methods are efficient for fingerprinting and genetic polymorphism studies of sphaerococcum mutant forms, and can illuminate the genome dynamics affecting the genes responsible for the sphaerococcum phenotype.

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Section: Bare-1/wis 2-1a Markers Are Efficient For Mutant Diversity Asupporting

confidence: 82%