Wheat seed ageing viewed through the cellular redox environment and changes in pH

Nagel, Manuela; Seal, Charlotte E.; Colville, Louise; Rodenstein, Axel; Un, Sun; Richter, Josefine; Pritchard, Hugh W.; Börner, Andreas; Kranner, Ilse

doi:10.1080/10715762.2019.1620226

Cited by 30 publications

(30 citation statements)

References 83 publications

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“…Left: Effect of ageing on Allium seedling phenology. Right: Pie charts presenting the relative proportions of normal and abnormal Allium seedlings environment, changes in pH, storage compounds and genotype (Salama and Pearce 1993;Selvi and Saraswathy 2018;Nagel et al 2019). Our findings with Allium seed ageing at elevated SMC demonstrate that the combination with either elevated pO 2 or temperature both cause rapid seed viability loss without appreciably affecting germination speed.…”

Section: Resultsmentioning

confidence: 68%

The effects of high oxygen partial pressure on vegetable Allium seeds with a short shelf-life

Hourston

Pérez

Gawthrop

et al. 2020

Planta

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Section: Resultsmentioning

confidence: 68%

The effects of high oxygen partial pressure on vegetable Allium seeds with a short shelf-life

Hourston

Pérez

Gawthrop

et al. 2020

Planta

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“…This desiccated state of seeds permits to keep the plant alive for long periods of time, until environmental conditions are favourable. However, dry seeds also experience deterioration and ageing, and it has been proposed that oxidative damage is a major cause for reducing longevity in this system (Bailly, 2004;Harman & Mattick, 1976;Nagel et al, 2019;Sano et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Identification of novel seed longevity genes related to oxidative stress and seed coat by genome‐wide association studies and reverse genetics

Renard

Niñoles

Martínez-Almonacid

et al. 2020

Plant Cell & Environment

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Seed longevity is a polygenic trait of relevance for agriculture and for understanding the effect of environment on the ageing of biological systems. In order to identify novel longevity genes, we have phenotyped the natural variation of 270 ecotypes of the model plant, Arabidopsis thaliana, for natural ageing and for three accelerated ageing methods. Genome‐wide analysis, using publicly available single‐nucleotide polymorphisms (SNPs) data sets, identified multiple genomic regions associated with variation in seed longevity. Reverse genetics of 20 candidate genes in Columbia ecotype resulted in seven genes positive for seed longevity (PSAD1, SSLEA, SSTPR, DHAR1, CYP86A8, MYB47 and SPCH) and five negative ones (RBOHD, RBOHE, RBOHF, KNAT7 and SEP3). In this uniform genetic background, natural and accelerated ageing methods provided similar results for seed‐longevity in knock‐out mutants. The NADPH oxidases (RBOHs), the dehydroascorbate reductase (DHAR1) and the photosystem I subunit (PSAD1) highlight the important role of oxidative stress on seed ageing. The cytochrome P‐450 hydroxylase, CYP86A8, and the transcription factors, MYB47, KNAT7 and SEP3, support the protecting role of the seed coat during seed ageing.

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“…In thiol-disulphide redox couples, the concentration of hydrogen ions affects the half-cell reduction potential (Ehc) (Wardman, 1989), therefore the cytoplasmic pH of control, CD-aged, and seed bank seeds was estimated as previously reported by (Nagel et al, 2019) with minor modifications. For each treatment, four replicates of 50.23 ± 0.52 mg of ground seed powder were suspended in 1.2 mL of UPW and shaken at 600 rpm and 100 °C for 10 min.…”

Section: Calculation Of Egssg/2gshmentioning

confidence: 99%

“…Following centrifugation (15,000 g, 30 min, RT), the supernatants were transferred to fresh Eppendorf tubes and their pH measured using a Multi 3410 pH meter with an ADA S7MDS electrode (VWR International, Wien, Austria). To account for acidification due to interfering compounds released from organelles during extraction of seed powder, a correction factor of +0.6, obtained as difference between the cellular physiological pH (7.30) and the highest pH measured in extracts of control seeds (6.70), was applied as detailed by (Nagel et al, 2019).…”

Section: Calculation Of Egssg/2gshmentioning

confidence: 99%

“…60-70%), ensuring fast declines of viability (Powell and Matthews, 1981;Hay et al, 2008). However, accelerating seed ageing using humid/warm conditions typical of CD does not always lead to the same biochemical changes that occur in dry/cold storage conditions of seed banks (Nagel et al, 2015;Roach et al, 2018a;Nagel et al, 2019). For example, viewed via a lack of changes in FA composition and tocochromanol concentrations, the lipid phase remains relatively stable during CD, even in some cases until viability loss under elevated O2 concentrations (Lehner et al, 2008;Morscher et al, 2015;Roach et al, 2018a;Schausberger et al, 2019), whereas tocochromanol consumption may occur during cold storage of oily and non-oily seeds (Seal et al, 2010a;Seal et al, 2010b;Roach et al, 2018a).…”

Section: Introductionmentioning

confidence: 99%

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Cytoplasmic physical state governs the influence of oxygen onPinus densifloraseed ageing

Gerna

Ballesteros

et al. 2020

Preprint

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During desiccation, the cytoplasm of orthodox seeds solidifies in a glass with highly restricted diffusion and molecular mobility, which extend longevity. Temperature and moisture determine seed cellular physical state, and oxygen can promote deteriorative reactions of seed ageing. However, whether seed physical state affects O2-mediated biochemical reactions during ageing remains unknown. Here, we answered this question using oil-rich Pinus densiflora seeds aged by controlled deterioration (CD) at 45 °C and distinct relative humidities (RHs), resulting in a glassy (9 and 33% RH) or fluid (64 and 85% RH) cytoplasm. Regardless of CD regimes, the cellular lipid domain remained always fluid. Hypoxia (0.4% O2) prevented seed deterioration only in the glassy state, limiting non-enzymatic lipid peroxidation, consumption of antioxidants (glutathione, tocopherols) and unsaturated fatty acids, accompanied by decreased lipid melt enthalpy and lower concentrations of aldehydes and reactive electrophile species (RES). In contrast, a fluid cytoplasm promoted faster seed deterioration and enabled the resumption of enzymatic activities implicated in glutathione metabolism and RES detoxification, regardless of O2 availability. Furthermore, seeds stored under dry/cold seed bank conditions showed biochemical profiles similar to those of CD-aged seeds with glassy cytoplasm under normoxia. These findings are discussed in the context of germplasm management.Highlightlipid peroxidation occurred during seed ageing in the glassy state and, like viability loss, could be prevented by hypoxia. Seeds with fluid cytoplasm aged faster and irrespective of oxygen availability.

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Wheat seed ageing viewed through the cellular redox environment and changes in pH

Cited by 30 publications

References 83 publications

The effects of high oxygen partial pressure on vegetable Allium seeds with a short shelf-life

The effects of high oxygen partial pressure on vegetable Allium seeds with a short shelf-life

Identification of novel seed longevity genes related to oxidative stress and seed coat by genome‐wide association studies and reverse genetics

Cytoplasmic physical state governs the influence of oxygen onPinus densifloraseed ageing

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