Wheat powdery mildew resistance gene Pm64 derived from wild emmer (Triticum turgidum var. dicoccoides) is tightly linked in repulsion with stripe rust resistance gene Yr5

Zhang, Deyun; Zhü, Kèyù; Dong, Lingli; Liang, Yong; Li, Genqiao; Fang, Tilin; Guo, Guanghao; Wu, Qiuhong; Xie, Jingzhong; Chen, Yongxing; Lü, Ping; Li, Miaomiao; Zhang, Huaizhi; Wang, Zhenzhong; Zhang, Yan; Sun, Qixin; Liu, Zhiyong

doi:10.1016/j.cj.2019.03.003

Cited by 70 publications

(30 citation statements)

References 52 publications

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“…Although more than a hundred powdery mildew (Pm) resistance genes/alleles in 63 loci (Pm1-Pm66) have been documented 6,7 , only seven of them, Pm2 8 , Pm3 9 , Pm8 10 , Pm21 11,12 , Pm38/Yr18/Lr34/Sr57 13 , Pm46/Yr46/Lr67/Sr55 14 , and Pm60 15 have been cloned and characterized so far. Most of these isolated Pm genes in wheat encode coiled coil nucleotide-binding leucinerich-repeat (NLR) proteins.…”

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A rare gain of function mutation in a wheat tandem kinase confers resistance to powdery mildew

et al. 2020

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Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is one of the most destructive diseases that pose a great threat to wheat production. Wheat landraces represent a rich source of powdery mildew resistance. Here, we report the map-based cloning of powdery mildew resistance gene Pm24 from Chinese wheat landrace Hulutou. It encodes a tandem kinase protein (TKP) with putative kinase-pseudokinase domains, designated WHEAT TANDEM KINASE 3 (WTK3). The resistance function of Pm24 was validated by transgenic assay, independent mutants, and allelic association analyses. Haplotype analysis revealed that a rare 6-bp natural deletion of lysine-glycine codons, endemic to wheat landraces of Shaanxi Province, China, in the kinase I domain (Kin I) of WTK3 is critical for the resistance function. Transgenic assay of WTK3 chimeric variants revealed that only the specific two amino acid deletion, rather than any of the single or more amino acid deletions, in the Kin I of WTK3 is responsible for gaining the resistance function of WTK3 against the Bgt fungus.

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A rare gain of function mutation in a wheat tandem kinase confers resistance to powdery mildew

et al. 2020

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“…Powdery mildew severely hinders yield and quality improvements in wheat and the loss of effective genetic resistance to powdery mildew due to the high variability of pathogens and the uniformity of resistance sources is leading to a crisis in wheat production [35][36][37]. Among the 92 powdery mildew resistance genes (Pm1~Pm65) that have been officially named, many are derived from wild relatives [38][39][40]. Thus, many studies have aimed to identify new resistance genes in the relatives of wheat [41,42].…”

Section: Discussionmentioning

confidence: 99%

A 1Ns Disomic Addition from Psathyrostachys Huashanica Keng Confers Resistance to Powdery Mildew in Wheat

Liu¹,

Hou²,

Li³

et al. 2020

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Powdery mildew is a fungal disease that threatens wheat production throughout the world. Breeding resistant cultivars is an effective way to reduce harm caused by powdery mildew. In this study, 35 wheat-Psathyrostachys huashanica-derived lines were developed by crossing common wheat and P. huashanica Keng (2n = 2x = 14, NsNs) using embryo culture. Resistance to powdery mildew in the derived lines was identified at the seedling and adult stages. Line H5-5-4-2 was selected with immunity to powdery mildew at both growth stages. The chromosome structure of this line was characterized by cytology, genomic in situ hybridization (GISH), and expressed sequence tag-sequence-tagged site (EST-STS) analysis. The chromosome configuration was 2n = 44 = 22II. Two P. huashanica chromosomes with strong hybridization signals were detected by GISH analysis. Among 83 EST-STS markers that covered all seven homologous groups in wheat, three pairs of STS markers, BE497584, BF202643, and BG262410, located in wheat homologous group 1 amplified clear specific bands related to P. huashanica. The results indicated that resistant line H5-5-4-2 was a wheat-P. huashanica 1Ns disomic addition line.

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“…Development and deployment of wheat varieties with resistance to powdery mildew is considered the most effective, economical, and environmentally friendly way to control losses caused by this disease [4,5]. Currently, up to 89 resistance genes/alleles, Pm1 to Pm65, are cataloged [6][7][8][9][10][11]. Some of these resistance genes, such as Pm8 derived from Secale cereale L. (2n = 2x = 14, RR), and Pm21, from Dasypyrum villosum (L.) P. Candargy (2n = 2x = 14, VV), have been used in wheat breeding programs worldwide [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Physical Mapping of Pm57, a Powdery Mildew Resistance Gene Derived from Aegilops searsii

Zhang

Tian

et al. 2020

IJMS

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Powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is one of many severe diseases that threaten bread wheat (Triticum aestivum L.) yield and quality worldwide. The discovery and deployment of powdery mildew resistance genes (Pm) can prevent this disease epidemic in wheat. In a previous study, we transferred the powdery mildew resistance gene Pm57 from Aegilops searsii into common wheat and cytogenetically mapped the gene in a chromosome region with the fraction length (FL) 0.75–0.87, which represents 12% segment of the long arm of chromosome 2Ss#1. In this study, we performed RNA-seq using RNA extracted from leaf samples of three infected and mock-infected wheat-Ae. searsii 2Ss#1 introgression lines at 0, 12, 24, and 48 h after inoculation with Bgt isolates. Then we designed 79 molecular markers based on transcriptome sequences and physically mapped them to Ae. searsii chromosome 2Ss#1- in seven intervals. We used these markers to identify 46 wheat-Ae. searsii 2Ss#1 recombinants induced by ph1b, a deletion mutant of pairing homologous (Ph) genes. After analyzing the 46 ph1b-induced 2Ss#1L recombinants in the region where Pm57 is located with different Bgt-responses, we physically mapped Pm57 gene on the long arm of 2Ss#1 in a 5.13 Mb genomic region, which was flanked by markers X67593 (773.72 Mb) and X62492 (778.85 Mb). By comparative synteny analysis of the corresponding region on chromosome 2B in Chinese Spring (T. aestivum L.) with other model species, we identified ten genes that are putative plant defense-related (R) genes which includes six coiled-coil nucleotide-binding site-leucine-rich repeat (CNL), three nucleotide-binding site-leucine-rich repeat (NL) and a leucine-rich receptor-like repeat (RLP) encoding proteins. This study will lay a foundation for cloning of Pm57, and benefit the understanding of interactions between resistance genes of wheat and powdery mildew pathogens.

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Wheat powdery mildew resistance gene Pm64 derived from wild emmer (Triticum turgidum var. dicoccoides) is tightly linked in repulsion with stripe rust resistance gene Yr5

Cited by 70 publications

References 52 publications

A rare gain of function mutation in a wheat tandem kinase confers resistance to powdery mildew

A rare gain of function mutation in a wheat tandem kinase confers resistance to powdery mildew

A 1Ns Disomic Addition from Psathyrostachys Huashanica Keng Confers Resistance to Powdery Mildew in Wheat

Physical Mapping of Pm57, a Powdery Mildew Resistance Gene Derived from Aegilops searsii

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