Wheat-Perennial Triticeae Introgressions: Major Achievements and Prospects

Ceoloni, Carla; Kuzmanović, Ljiljana; Forte, Paola; Virili, Maria Elena; Bitti, Alessandra

doi:10.1007/978-3-319-23494-6_11

Cited by 27 publications

(34 citation statements)

References 194 publications

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“…Numerous genes providing tolerance to biotic stress (i.e., resistance to barley yellow dwarf virus, wheat streak mosaic virus, yellow rust, leaf rust, stem rust, and powdery mildew) and abiotic stresses (i.e., cold‐, salinity‐ and drought tolerance) as well as genes improving yield were identified in crested wheatgrass (Shukle et al, 1987; Brettell et al, 1988; Littlejohn, 1988; Whelan, 1988; Friebe et al, 1992; Luan et al, 2010; Song et al, 2013; Ceoloni et al, 2015; Ochoa et al, 2015). In the present study, we extended the chromosome‐based approach to the complex P‐genome of crested wheatgrass (Said et al, 2018a) to produce genomic resources for chromosome‐mediated gene transfer into wheat.…”

Section: Discussionmentioning

confidence: 99%

“…Beside annual species, the Triticeae tribe includes ∼400 perennials commonly referred to as wheatgrass and wildrye. Several chromosomal reorganizations have occurred during the evolution resulting in diverse genetic architecture of this cross‐pollinating species (Ceoloni et al, 2015). Five major genomic types (St, P, Ns, J, and E) can be discriminated in the diploid and polyploid species.…”

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confidence: 99%

“…Depending on how the genus was treated taxonomically, up to fifteen species have identified in the genus Agropyron (Löve, 1982, 1984; Li et al, 2007; Wang, 2011; Linc et al, 2017). Agropyron species are considered as highly valuable sources to improve the crop resistance to biotic and abiotic stresses as well as quality and yield‐related traits (Ceoloni et al, 2015). One of the most widely spread species is crested wheatgrass, which is distributed in Eurasia (Dewey, 1984; Yang et al, 2014; Zhao et al, 2017).…”

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Dissecting the Complex Genome of Crested Wheatgrass by Chromosome Flow Sorting

et al. 2019

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Core Ideas Flow sorting enabled to dissect the genome of crested wheatgrass into chromosomes. This is a powerful approach to access the complex genome of a wheat wild relative. Chromosome genomics will support alien introgression breeding of the crop. Wheatgrass (Agropyron sp.) is a potential source of beneficial traits for wheat improvement. Among them, crested wheatgrass [A. cristatum (L.) Gaertn.] comprises a complex of diploid, tetraploid, and hexaploid forms with the basic genome P, with some accessions carrying supernumerary B chromosomes (Bs). In this work, we applied flow cytometry to dissect the complex genome of crested wheatgrass into individual chromosomes to facilitate its analysis. Flow karyotypes obtained after the analysis of 4ʹ,6‐diamidino‐2‐phenylindole (DAPI)‐stained mitotic chromosomes of diploid and tetraploid accessions consisted of three peaks, each corresponding to a group of two or three chromosomes. To improve the resolution, bivariate flow karyotyping after fluorescent labeling of chromosomes with fluorescein isothiocyanate (FITC)‐conjugated probe (GAA)7 microsatellite was applied and allowed discrimination and sorting of P genome chromosomes from wheat–crested wheatgrass addition lines. Chromosomes 1P–6P and seven telomeric chromosomes could be sorted at purities ranging from 81.7 to 98.2% in disomics and from 44.8 to 87.3% in telosomics. Chromosome 7P was sorted at purities reaching 50.0 and 39.5% in diploid and tetraploid crested wheatgrass, respectively. In addition to the whole complement chromosomes (A), Bs could be easily discriminated and sorted from a diploid accession at 95.4% purity. The sorted chromosomes will streamline genome analysis of crested wheatgrass, facilitating gene cloning and development of molecular tools to support alien introgression into wheat.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Dissecting the Complex Genome of Crested Wheatgrass by Chromosome Flow Sorting

et al. 2019

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“…Various beneficial genes can thus be accumulated, and unwanted genes possibly eliminated. Particularly noteworthy examples of this strategy concern gene combinations from perennial Triticeae species of the Thinopyrum genus, one of the richest sources of valuable genes/QTL for wheat improvement [36,37]. By means of ph1b-mediated recombination between a bread wheat-Th.…”

Section: Assembling Genes From Different Alien Sources Into the Same mentioning

confidence: 99%

Harnessing Genetic Diversity of Wild Gene Pools to Enhance Wheat Crop Production and Sustainability: Challenges and Opportunities

et al. 2017

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Wild species are extremely rich resources of useful genes not available in the cultivated gene pool. For species providing staple food to mankind, such as the cultivated Triticum species, including hexaploid bread wheat (Triticum aestivum, 6x) and tetraploid durum wheat (T. durum, 4x), widening the genetic base is a priority and primary target to cope with the many challenges that the crop has to face. These include recent climate changes, as well as actual and projected demographic growth, contrasting with reduction of arable land and water reserves. All of these environmental and societal modifications pose major constraints to the required production increase in the wheat crop. A sustainable approach to address this task implies resorting to non-conventional breeding strategies, such as "chromosome engineering". This is based on cytogenetic methodologies, which ultimately allow for the incorporation into wheat chromosomes of targeted, and ideally small, chromosomal segments from the genome of wild relatives, containing the gene(s) of interest. Chromosome engineering has been successfully applied to introduce into wheat genes/QTL for resistance to biotic and abiotic stresses, quality attributes, and even yield-related traits. In recent years, a substantial upsurge in effective alien gene exploitation for wheat improvement has come from modern technologies, including use of molecular markers, molecular cytogenetic techniques, and sequencing, which have greatly expanded our knowledge and ability to finely manipulate wheat and alien genomes. Examples will be provided of various types of stable introgressions, including pyramiding of different alien genes/QTL, into the background of bread and durum wheat genotypes, representing valuable materials for both species to respond to the needed novelty in current and future breeding programs. Challenging contexts, such as that inherent to the 4x nature of durum wheat when compared to 6x bread wheat, or created by presence of alien genes affecting segregation of wheat-alien recombinant chromosomes, will also be illustrated.

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“…There is not complete agreement, but cytogenetic investigations have shown there to be ~10 basic genomes in the perennial species of Triticeae on the basis of homology (Ceoloni et al, 2015). Combinations and repetitions of these basic genomes, with varying degrees of homoeology, make up the variation represented by these wild species, with ploidy ranging from diploid to dodecaploid.…”

Section: Wild Relatives Of Triticummentioning

confidence: 99%

Breeding Perennial Grain Crops Based on Wheat

Curwen-McAdams

Jones

2017

Crop Science

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Wild relatives offer a diversity of traits potentially useful in crop improvement. Identification of major genes for resistance to biotic and abiotic stresses, in conjunction with targeted introgression, has introduced some of these traits into cultivated varieties. The difficulty of transferring complex traits, and associated linkage drag, has limited the ability of breeding programs to fully use important genetic variation. Wheat (Triticum L.) is an example of an important crop with large secondary and tertiary genepools of species capable of producing viable offspring by cross pollination. Wheat is annual, but most of the related species in the Triticeae Dumort tribe are perennials. An unknown number of genes control the perennial habit, although previous work has shown that it is a complex trait with quantitative expression. Lack of recombination between the parental genomes and complexity of the trait suggest another avenue for breeding: the addition of a genome. Multiple genera can hybridize with wheat, and some combinations produce stable amphiploids. The development of perennial grain and forage crops balancing the agronomics of wheat with the polycarpic habit of its relatives is promising. The approach is similar to Triticale (× Triticosecale Wittmack ex A. Camus), where new crops are developed combining the desirable qualities of the parents. Through prebreeding of the wild relatives, application of insight into meiosis, and strategic use of known genes, it should be more efficient to breed perennial grain crops adapted to diverse production systems.

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Wheat-Perennial Triticeae Introgressions: Major Achievements and Prospects

Cited by 27 publications

References 194 publications

Dissecting the Complex Genome of Crested Wheatgrass by Chromosome Flow Sorting

Dissecting the Complex Genome of Crested Wheatgrass by Chromosome Flow Sorting

Harnessing Genetic Diversity of Wild Gene Pools to Enhance Wheat Crop Production and Sustainability: Challenges and Opportunities

Breeding Perennial Grain Crops Based on Wheat

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